Citation: LIN Tao, SHAO Jinliang, LIU Xingyong, YANG Dongshun, CHEN Xinglian, LI Yangang, FAN Jianlin, LIU Hongcheng. Determination of 41 pesticide residues in vegetables by QuEChERS-ultra performance liquid chromatography- tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2015, 33(3): 235-241. doi: 10.3724/SP.J.1123.2014.11023 shu

Determination of 41 pesticide residues in vegetables by QuEChERS-ultra performance liquid chromatography- tandem mass spectrometry

1.
1. Institute of Agriculture Quality Standards & Testing Technique, Yunnan Academy of Agricultural Science, Kunming 650223, China;

Corresponding author: LIU Hongcheng,
Received Date: 17 November 2014
Available Online: 25 December 2014
Fund Project: 2014年国家蔬菜产品质量安全风险评估项目(GJFP2014001) (GJFP2014001)云南省社会发展科技计划项目(2012CA005). (2012CA005)

A multiresidue analytical method for the rapid determination of 41 pesticide residues in vegetables was developed by using QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), including recessive pesticides, banned pesticides and pesticides with restrict use, plant growth regulators and some other pesticides with high detection rates. The vegetable sample was extracted by 1% (v/v) acetic acid-acetonitrile, cleaned-up by primary secondary amine (PSA) and injected for analysis. The positive and negative ion modes and multiple reaction monitoring (MRM) mode were used in the analysis, and the analytes were quantified by the external standard method. Under the conditions of optimized QuEChERS, chromatography and mass spectrometry, the 41 pesticides showed good linearities in the range of 1.0 μg/L to 100 μg/L, with the correlation coefficients (r²) higher than 0.999. The limits of detection of the method were 0.003 μg/kg to 1.00 μg/kg. The average recoveries of the 41 pesticides in different matrices were in the range of 74.1%-120.4% with the relative standard deviations from 2.8% to 11. 9%. The method has the advantages of rapidity, simplicity, high sensitivity and better purification effect. It is suitable for the rapid determination of the common pesticides in vegetables, and it provides a strong guarantee for the risk assessment of the quality and safety of vegetables.
- QuEChERS,
- ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS),
- pesticides,
- vegetable,
- residues
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沈阳化工大学材料科学与工程学院沈阳 110142