Citation: YANG Huamei, HANG Li. Determination of four frequently-used essences in foods by ultra performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2015, 33(3): 250-255. doi: 10.3724/SP.J.1123.2014.11018 shu

Determination of four frequently-used essences in foods by ultra performance liquid chromatography-tandem mass spectrometry

YANG Huamei ^, ,
HANG Li

1.
Taizhou Center for Disease Control and Prevention, Taizhou 225300, China

Corresponding author: YANG Huamei,
Received Date: 13 November 2014
Available Online: 6 January 2015

A method has been developed for the simultaneous determination of vanillin, ethyl vanillin, maltol and ethyl maltol in foods by ultra performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS). The sample was extracted with ultrapure water, and purified with SPE column. The target compounds were separated on a C18 column with the gradient elution of methanol and water containing 0.002 mol/L ammonium acetate and 0.1%(v/v) formic acid. The four components were determined in the modes of electrospray positive ionization (ESI⁺) and multiple reaction monitoring (MRM). The linear ranges were 10-1000 μg/L for vanillin and maltol and 5-500 μg/L for ethyl vanillin and ethyl maltol, with the correlation coefficients more than 0.999. The recoveries of the four compounds ranged from 75.8% to 116%, with the relative standard deviations (RSDs) of 1.58%-4.01%. The method showed good extraction efficiency, high sensitivity and good reproducibility. It is suitable for the simultaneous detection of vanillin, ethyl vanillin, maltol and ethyl maltol in foods.
- ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS),
- vanillin,
- ethyl vanillin,
- maltol,
- ethyl vanillin,
- food
1. [1]
  [1] Liu S T, Liu Y H, Ruan R S, et al. Science and Technology of Food Industry (刘士涛, 刘玉环, 阮榕生, 等. 食品工业科技), 2012(9): 426
2. [2]
  [2] Chen J M. Food Research and Development (陈金明. 食品研究与开发), 2004, 25(1): 34
3. [3]
  [3] Pan L. Journal of Hunan City University: Natural Science (潘磊. 湖南城市学院学报: 自然科学版), 2011, 20(2): 48
4. [4]
  [4] Hironishi M, Kordek R, Yanagihara R, et al. Neurodegeneration, 1996, 5(4): 325
5. [5]
  [5] Liang R F, Niu Q. Journal of Toxicology (梁瑞峰, 牛侨. 毒理学杂志), 2011, 25(6): 467
6. [6]
  [6] Liang R F, Li W Q, Niu Q. Journal of Environment and Health (梁瑞峰, 李伟庆, 牛侨. 环境与健康杂志), 2011, 28 (4): 302
7. [7]
  [7] Zhang G W, Ni Y N. Journal of Analytical Science (张国文, 倪永年. 分析科学学报), 2005, 21(1): 20
8. [8]
  [8] Ni Y N, Wang P P. Journal of Nanchang University: Natural Science (倪永年, 王苹苹. 南昌大学学报: 理科版), 2012, 36(1): 43
9. [9]
  [9] Zhou J, Di J W, Wu Y, et al. Chinese Journal of Applied Chemistry (周君, 狄俊伟, 吴莹, 等. 应用化学), 2008, 25(1): 81
10. [10]
  [10] Peng S H, Ma W H, Di J W. Chinese Journal of Spectroscopy Laboratory (彭少华, 马文华, 狄俊伟. 光谱实验室), 2005, 22(4): 680
11. [11]
  [11] Wang H, Liu Y Q, Yang H M, et al. Food Science and Technology (王浩, 刘艳琴, 杨红梅, 等. 食品科技), 2006(8): 235
12. [12]
  [12] Jing L J, Han W, Yan G L. Food Science (景丽洁, 韩伟, 闫光烈. 食品科学), 2006, 27(4): 205
13. [13]
  [13] Farthing D, Sica D, Abernathy C, et al. J Chromatogr B, 1999, 726: 303
14. [14]
  [14] Waliszewski K N, Pardio V T, Ovando S L. Food Chem, 2006, 101: 1059
15. [15]
  [15] Li H F, Yang H Y, Zhang Y, et al. Chinese Journal of Chromatography (李海芳, 杨红云, 张英, 等. 色谱), 2014, 32(4): 413
16. [16]
  [16] Sanfeliu Alonso M C, Lahuerta Zamora L, Martinez Calatayud J. Anal Chim Acta, 2001, 438: 157
17. [17]
  [17] de Jager L S, Perfetti G A, Diachenko G W. Food Chem, 2008, 107: 1701
18. [18]
  [18] Li X Y, Wang H. Farm Machinery (李小运, 王浩. 农业机械), 2012(3): 126
19. [19]
  [19] de Jager L S, Perfetti G A, Diachenko G W. J Chromatogr A, 2007, 1145: 83
20. [20]
  [20] Ohashi M, Omae H, Hashida M. J Chromatogr A, 2007, 1138: 262
21. [21]
  [21] Ren H B, Bie Z Y, Wei Y P, et al. Chinese Journal of Analysis Laboratory (任呼博, 别振英, 蔚亦沛, 等. 分析试验室), 2014, 33(8): 950
22. [22]
  [22] Ni Y N, Zhang G W, Serge K. Food Chem, 2005, 89(3): 465
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沈阳化工大学材料科学与工程学院沈阳 110142