Citation: HUANG Daihong, ZHANG Zhenguo, CHEN Guoping, LI Houhun, SHI Fuchen. Analysis of the components of floral scent in Glochidion puberum using gas chromatography-mass spectrometry with dynamic headspace adsorption[J]. Chinese Journal of Chromatography, ;2015, 33(3): 318-322. doi: 10.3724/SP.J.1123.2014.11005 shu

Analysis of the components of floral scent in Glochidion puberum using gas chromatography-mass spectrometry with dynamic headspace adsorption

1.
College of Life Science, Nankai University, Tianjin 300071, China

Corresponding author: SHI Fuchen,
Received Date: 5 November 2014
Available Online: 15 December 2014
Fund Project: 国家自然科学基金重点项目(30930014). (30930014)

The floral scent plays the important key role in maintaining the obligate pollination mutualism between Glochidion plants and Epicephala moths. In the study, the dynamic headspace adsorption technique was employed to collect the floral scent emitted by Glochidion puberum, gas chromatography coupled with mass spectrometry (GC-MS) was used for the detection and identification of volatile chemical components in headspace samples of flowers from G. puberum. The peak area normalization was used to determine the relative contents of each odour component. The results showed that 45 compounds mainly consisting of monoterpenes and sesquiterpenes were isolated from the floral scent produced by G. puberum. Especially, both linalool (38.06%) and β-elemene (23.84%) were considered as the major scent components of G. puberum. It was speculated that linalool and β-elemene may be the two potential compounds attracting female Epicephala moths. The study provided the basic data for further electroantennographic detection and bioassays to identify the compounds having the actual physiological activity to female Epicephala moths.
- dynamic headspace adsorption (DHSA),
- gas chromatography-mass spectrometry (GC-MS),
- floral scent,
- Glochidion puberum,
- mutualism
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