Citation: WANG Xiyue, GAO Peng, XU Guowang. Hydrophilic interaction liquid chromatography coupled with mass spectrometry for metabolomic analysis of Escherichia coli[J]. Chinese Journal of Chromatography, ;2014, 32(10): 1084-1093. doi: 10.3724/SP.J.1123.2014.08008 shu

Hydrophilic interaction liquid chromatography coupled with mass spectrometry for metabolomic analysis of Escherichia coli

1.
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China

Corresponding author: GAO Peng,
Received Date: 11 August 2014

The zwitterionic hydrophilic interaction liquid chromatography coupled with mass spectrometry (ZIC-HILIC/MS) method was developed and applied for studying the metabolome of Escherichia coli. In this method, the linear ranges of the 52 representative compounds were between 2-6 orders of magnitude. Moreover the lowest limits of detection of most compounds were at ng/mL levels. The analysis for six samples prepared in parallel showed that the method had good reproducibility with more than 85% metabolites with the RSDs of peak area less than 30%. The precision of the method was tested by evaluating the relative recoveries of six internal standards at the low, middle and high concentrations. The results exhibited that the intraday precisions (RSDs) of the six compounds in the same concentrations were less than 20%. The recoveries of most compounds were accepted in the range of 70%-130%. The results of metabolomic analysis of three Escherichia coli strains with genetic modified yfcC gene showed that some small peptides, amino acids, nucleosides, organic acids and phospholipids changed differentially after the yfcC gene modification. These results indicated that the ZIC-HILIC/MS method could detect diverse metabolites and be used for metabolomic analysis purpose with good reproducibility and stability.
1. [1]
  [1] Nicholson J K, Lindon J C, Holmes E. Xenobiotica, 1999, 29(11): 1181
2. [2]
  [2] Fiehn O. Plant Mol Biol, 2002, 48(1/2): 155
3. [3]
  [3] Altmaier E, Emeny R T, Krumsiek J, et al. Psychoneuroendocrinology, 2013, 38(8): 1299
4. [4]
  [4] Chen J, Wang W Z, Lv S, et al. Anal Chim Acta, 2009, 650(1): 3
5. [5]
  [5] He H B, Shi X Z, Chen J, et al. Chinese Journal of Chromatography (和红兵, 石先哲, 陈静, 等. 色谱), 2012, 30(3): 245
6. [6]
  [6] Yu X W, Wu Q, Lü W, et al. Chinese Journal of Chromatography (余欣尉, 吴谦, 吕望, 等. 色谱), 2013, 31(7): 691
7. [7]
  [7] Zhou J, Zhang L, Chang Y W, et al. J Proteome Res, 2012, 11(8): 4351
8. [8]
  [8] Zeng J, Kuang H, Hu C X, et al. Environ Sci Technol, 2013, 47: 7457
9. [9]
  [9] Chen M J, Zhou K, Chen X J, et al. Toxicol Sci, 2014, 138(2): 256
10. [10]
  [10] Gu Y, Zhang Y F, Shi X Z, et al. Talanta, 2010, 81(3): 766
11. [11]
  [11] Ohashi Y, Hirayama A, Ishikawa T, et al. Mol Biosyst, 2008, 4(2): 135
12. [12]
  [12] Wang Q Z, Wu C Y, Chen T, et al. Appl Microbiol Biotechnol, 2006, 70(2): 151
13. [13]
  [13] Li Y, Cao Z A. Journal of Chemical Industry and Engineering (李寅, 曹竹安. 化工学报), 2004, 55(10): 1574
14. [14]
  [14] Sprenger G A. Appl Microbiol Biotechnol, 2007, 75(4): 739
15. [15]
  [15] Janen H J, Steinbüchel A. Biotechnol Biofuels, 2014, 7(7), DOI:10.1186/1754-6834-7-7
16. [16]
  [16] Shah P, Chiu F S, Lan J C W. J Biosci Bioeng, 2014, 117(3): 343
17. [17]
  [17] Zhu L W, Li X H, Zhang L, et al. Metab Eng, 2013, 20: 9
18. [18]
  [18] Bennett B D, Kimball E H, Gao M, et al. Nat Chem Biol, 2009, 5(8): 593
19. [19]
  [19] Garcia D E, Baidoo E E, Benke P I, et al. Curr Opin Microbiol, 2008, 11(3): 233
20. [20]
  [20] Wittmann C. Microb Cell Fact, 2007, 6(6), DOI:10.1186/1475-2859-6-6
21. [21]
  [21] Buscher J M, Czernik D, Ewald J C, et al. Anal Chem, 2009, 81(6): 2135
22. [22]
  [22] Bajad S U, Lu W, Kimball E H, et al. J Chromatogr A, 2006, 1125(1): 76
23. [23]
  [23] Preinerstorfer B, Schiesel S, Lammerhofer M, et al. J Chromatogr A, 2010, 1217(3): 312
24. [24]
  [24] Yuan M, Breitkopf S B, Yang X, et al. Nat Protoc, 2012, 7(5): 872
25. [25]
  [25] Wang X Y, Xie Y P, Gao P, et al. Anal Biochem, 2014, 451: 48
26. [26]
  [26] Smith C A, Elizabeth J, O'Maille G, et al. Anal Chem, 2006, 78(3): 779
1. [1]
  Qin Tu , Anju Tao , Tongtong Ma , Jinyi Wang . Innovative Experimental Teaching of Escherichia coli Detection Based on Paper Chip. University Chemistry, 2024, 39(6): 271-277. doi: 10.3866/PKU.DXHX202309062
2. [2]
  Qingyang Cui , Feng Yu , Zirun Wang , Bangkun Jin , Wanqun Hu , Wan Li . From Jelly to Soft Matter: Preparation and Properties-Exploring of Different Kinds of Hydrogels. University Chemistry, 2024, 39(9): 338-348. doi: 10.3866/PKU.DXHX202309046
3. [3]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
4. [4]
  Xiaohui Li , Ze Zhang , Jingyi Cui , Juanjuan Yin . Advanced Exploration and Practice of Teaching in the Experimental Course of Chemical Engineering Thermodynamics under the “High Order, Innovative, and Challenging” Framework. University Chemistry, 2024, 39(7): 368-376. doi: 10.3866/PKU.DXHX202311027
5. [5]
  Zhuomin Zhang , Hanbing Huang , Liangqiu Lin , Jingsong Liu , Gongke Li . Course Construction of Instrumental Analysis Experiment: Surface-Enhanced Raman Spectroscopy for Rapid Detection of Edible Pigments. University Chemistry, 2024, 39(2): 133-139. doi: 10.3866/PKU.DXHX202308034
6. [6]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
7. [7]
  Chi Zhang , Yi Xu , Xiaopeng Guo , Zian Jie , Ling Li . 五彩斑斓的秘密——物质显色机理. University Chemistry, 2025, 40(6): 266-275. doi: 10.12461/PKU.DXHX202407061
8. [8]
  Yuhang Jiang , Weijie Liu , Jiaqi Cai , Jiayue Chen , Yanping Ren , Pingping Wu , Liulin Yang . A Journey into the Science and Art of Sugar: “Dispersion of Light and Optical Rotation of Matter” Science Popularization Experiment. University Chemistry, 2024, 39(9): 288-294. doi: 10.12461/PKU.DXHX202401054
9. [9]
  Zhifang SU , Zongjie GUAN , Yu FANG . Process of electrocatalytic synthesis of small molecule substances by porous framework materials. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2373-2395. doi: 10.11862/CJIC.20240290
10. [10]
  Kuaibing Wang , Feifei Mao , Weihua Zhang , Bo Lv . Design and Practice of a Comprehensive Teaching Experiment for Preparing Biomass Carbon Dots from Rice Husk. University Chemistry, 2025, 40(5): 342-350. doi: 10.12461/PKU.DXHX202407042
11. [11]
  Tianyang Yu , Hao Wei . “Illness Enters through the Mouth”: A Brief Overview of Toxic Chemical Substances in Common Foods. University Chemistry, 2025, 40(7): 225-231. doi: 10.12461/PKU.DXHX202409083
12. [12]
  . . Chinese Journal of Inorganic Chemistry, 2024, 40(11): 0-0.
13. [13]
  Yinuo Wang , Siran Wang , Yilong Zhao , Dazhen Xu . Selective Synthesis of Diarylmethyl Anilines and Triarylmethanes via Multicomponent Reactions: Introduce a Comprehensive Experiment of Organic Chemistry. University Chemistry, 2024, 39(8): 324-330. doi: 10.3866/PKU.DXHX202401063
14. [14]
  Lu Zhuoran , Li Shengkai , Lu Yuxuan , Wang Shuangyin , Zou Yuqin . Cleavage of C―C Bonds for Biomass Upgrading on Transition Metal Electrocatalysts. Acta Physico-Chimica Sinica, 2024, 40(4): 2306003-0. doi: 10.3866/PKU.WHXB202306003
15. [15]
  Lei Shi . Nucleophilicity and Electrophilicity of Radicals. University Chemistry, 2024, 39(11): 131-135. doi: 10.3866/PKU.DXHX202402018
16. [16]
  Jingming Li , Bowen Ding , Nan Li , Nurgul . Application of Comparative Teaching Method in Experimental Project Design of Instrumental Analysis Course: A Case Study in Chromatography Experiment Teaching. University Chemistry, 2024, 39(8): 263-269. doi: 10.3866/PKU.DXHX202312078
17. [17]
  Yanglin Jiang , Mingqing Chen , Min Liang , Yige Yao , Yan Zhang , Peng Wang , Jianping Zhang . Experimental and Theoretical Investigations of Solvent Polarity Effect on ESIPT Mechanism in 4′-N,N-diethylamino-3-hydroxybenzoflavone. Acta Physico-Chimica Sinica, 2025, 41(2): 100012-0. doi: 10.3866/PKU.WHXB202309027
18. [18]
  Jiaxun Wu , Mingde Li , Li Dang . The R eaction of Metal Selenium Complexes with Olefins as a Tutorial Case Study for Analyzing Molecular Orbital Interaction Modes. University Chemistry, 2025, 40(3): 108-115. doi: 10.12461/PKU.DXHX202405098
19. [19]
  Huiying Xu , Minghui Liang , Zhi Zhou , Hui Gao , Wei Yi . Application of Quantum Chemistry Computation and Visual Analysis in Teaching of Weak Interactions. University Chemistry, 2025, 40(3): 199-205. doi: 10.12461/PKU.DXHX202407011
20. [20]
  Qiuting Zhang , Fan Wu , Jin Liu , Zian Lin . Chromatographic Stationary Phase and Chiral Separation Using Frame Materials. University Chemistry, 2025, 40(4): 291-298. doi: 10.12461/PKU.DXHX202405174

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(479)
HTML views(18)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142