Citation: GENG Yue, SUN Fengxia, MA Yu, DENG Ligang, LÜ Jianyun, LI Teng, WANG Congcong. Serum metabolomics analysis on benign prostate hyperplasia in mice based on liquid chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(12): 1301-1305. doi: 10.3724/SP.J.1123.2014.08005 shu

Serum metabolomics analysis on benign prostate hyperplasia in mice based on liquid chromatography-mass spectrometry

GENG Yue ^1,, ,
SUN Fengxia ¹ ,
MA Yu ¹ ,
DENG Ligang ² ,
LÜ Jianyun ¹ ,
LI Teng ² ,
WANG Congcong ²

1.
1. Provincial Key Laboratory of Animal Resistant Biology, College of Life Science, Shandong Normal University, Jinan 250014, China;

Corresponding author: GENG Yue,
Received Date: 11 August 2014
Available Online: 16 September 2014

Benign prostatic hyperplasia (BPH) increasingly becomes a common factor affecting the quality of life of aging men. Its pathogenesis has not yet been fully elucidated. Ultra-high pressure liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) was employed to detect the changes of serum metabolites in normal mice, benign prostatic hyperplasia model mice and BPH model mice with finasteride intervention. The serum metabolite profiles of the three groups of mice were analyzed. Partial least squares-discriminant analysis (PLS-DA) was used for group differentiation and biomarker selection. The results showed good distinction among the three groups of mice serum metabolite spectra. Three potential biomarkers, 1-hexadecanoyl-SN-glycero-3-phosphocholine, 1-O-hexadecyl-2-O-acetyl-sn-glyceryl-3-phosphorylcholine and (Z)-13-docosenamide, were discovered and identified. They all indicated the occurrence of benign prostatic hypertrophy is closely related to the disorders of lipid metabolism. Compared with the control group, the contents of the first two substances were significantly increased in the serum of BPH model mice, and significantly decreased after intervened by finasteride. The contents of (Z)-13-docosenamide decreased significantly in the serum of model group, and increased after intervened by finasteride. Compared with the control group, the contents of three biomarkers in finasteride group did not recover completely and had significant differences. This study is conductive to open new avenues of diagnosis and medical treatment for BPH.
1. [1]
  [1] Shin I S, Lee M Y, Lim H S, et al. J Korean Soc Biol Chem, 2012, 55(2): 205
2. [2]
  [2] Wu N, Sun J, Yu P, et al. Int Neurourol J, 2012, 16(4): 191
3. [3]
  [3] Zhang X M, Zhang L H, Zhang Y K. Chinese Journal of Chromatography (张祥民, 张丽华, 张玉奎. 色谱), 2012, 30(3): 222
4. [4]
  [4] Wu H, Liu T, Ma C, et al. Anal Bioanal Chem, 2011, 401(2): 635
5. [5]
  [5] Saylor P J, Karoly E D, Smith M R. Clin Cancer Res, 2012, 18(13): 3677
6. [6]
  [6] Thysell E, Surowiec I, Hörnberg E, et al. PLoS One, 2010, 5(12): e14175
7. [7]
  [7] Bijlsma S, Bobeldijk I, Verheij E R, et al. Anal Chem, 2006, 78(2): 567
8. [8]
  [8] Li L, Luo G A, Lu C J, et al. Journal of Modern Oncology (黎莉, 罗国安, 卢传坚, 等. 现代肿瘤医学), 2011, 19(12): 2361
9. [9]
  [9] Jia D S, Hu W Y. Journal of Clinical Transfusion and Laboratory Medicine (贾道山, 胡文英. 临床输血与检验), 2003, 5(4): 312
10. [10]
  [10] Murphy A B, Macejko A. Drugs, 2009, 69(1): 71
11. [11]
  [11] Yuan Q, Zhang X H, Qiao Y J. China Journal of Traditional Chinese Medicine and Pharmacy (袁奇, 张小华, 乔延江. 中华中医药杂志), 2011, 26(7): 1568
12. [12]
  [12] Bartsch G, Rittmaster R S, Klocker H. Der Urologe Ausg A, 2002, 41(5): 412
13. [13]
  [13] Hai Q M, Shen D L, Liu Z L, et al. Henan Medical Research (海祺旻, 沈德良, 刘琮琳, 等. 河南医学研究), 2014, 23(1): 19
14. [14]
  [14] Jiang L. Journal of Community Medicine (蒋黎. 社区医学杂志), 2014(4): 16
15. [15]
  [15] Li Z W, Wang Z J, Xu X, et al. Journal of Clinical Emergency Call (李志伟, 王著军, 徐旭, 等. 临床急诊杂志), 2013(8): 363
16. [16]
  [16] Sakr M, Eid A, Shoukry M, et al. Bju Int, 2008, 101(3): 2
17. [17]
  [17] Geppetti P, Nassini R, Materazzi S, et al. Bju Int, 2008, 101(s3): 2
18. [18]
  [18] Ackerstaff E, Pflug B R, Nelson J B, et al. Cancer Res, 2001, 61(9): 3599
19. [19]
  [19] Glunde K, Jie C, Bhujwalla Z M. Cancer Res, 2004, 64(12): 4270
20. [20]
  [20] Mills G B, Moolenaar W H. Nat Rev Cancer, 2003, 3(8): 582
21. [21]
  [21] Zhao Z, Xiao Y, Elson P, et al. J Clin Oncol, 2007, 25(19): 2696
22. [22]
  [22] Kang A C, Huo Y, Qi L T. Chinese Journal of Arteriosclerosis (亢爱春, 霍勇, 齐丽彤. 中国动脉硬化杂志), 2006, 14(12): 1083
23. [23]
  [23] Liu J F, Deng M, Hou Y N. Medical Journal of National Defending Forces in North China (刘建芳, 邓鸣, 侯艳宁. 华北国防医药), 2006, 18(4): 286
24. [24]
  [24] Lan R Z, Hu Z Q, Zhuang Q Y. Acta Medicinae Universitatis Scientiae et Technologiae Huazhong (蓝儒竹, 胡志全, 庄乾元. 华中科技大学学报: 医学版), 2009, 38(5): 703
25. [25]
  [25] Hamberger A, Stenhagen G. Neurochem Res, 2003, 28(2): 177
26. [26]
  [26] Xin Z X, Wang D W, Sheng C, et al. Progress in Modern Biomedicine (忻志祥, 王大伟, 盛畅, 等. 现代生物医学进展), 2012, 12(18): 3538
27. [27]
  [27] Hieble J P. Drug Discov Today, 2004, 1(2): 243
28. [28]
  [28] Zhang J S, Liu J L, Liu N, et al. Beijing Medical Journal (张进生, 柳建垒, 刘宁, 等. 北京医学), 2014, 36(4): 292
29. [29]
  [29] Liu Q B. Journal of Qiqihar Medical College (刘巧斌. 齐齐哈尔医学院学报), 2013, 33(22): 3041
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