Citation: GENG Yue, SUN Fengxia, MA Yu, DENG Ligang, LÜ Jianyun, LI Teng, WANG Congcong. Serum metabolomics analysis on benign prostate hyperplasia in mice based on liquid chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(12): 1301-1305. doi: 10.3724/SP.J.1123.2014.08005 shu

Serum metabolomics analysis on benign prostate hyperplasia in mice based on liquid chromatography-mass spectrometry

GENG Yue ^1,, ,
SUN Fengxia ¹ ,
MA Yu ¹ ,
DENG Ligang ² ,
LÜ Jianyun ¹ ,
LI Teng ² ,
WANG Congcong ²

1.
1. Provincial Key Laboratory of Animal Resistant Biology, College of Life Science, Shandong Normal University, Jinan 250014, China;

Corresponding author: GENG Yue,
Received Date: 11 August 2014
Available Online: 16 September 2014

Benign prostatic hyperplasia (BPH) increasingly becomes a common factor affecting the quality of life of aging men. Its pathogenesis has not yet been fully elucidated. Ultra-high pressure liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) was employed to detect the changes of serum metabolites in normal mice, benign prostatic hyperplasia model mice and BPH model mice with finasteride intervention. The serum metabolite profiles of the three groups of mice were analyzed. Partial least squares-discriminant analysis (PLS-DA) was used for group differentiation and biomarker selection. The results showed good distinction among the three groups of mice serum metabolite spectra. Three potential biomarkers, 1-hexadecanoyl-SN-glycero-3-phosphocholine, 1-O-hexadecyl-2-O-acetyl-sn-glyceryl-3-phosphorylcholine and (Z)-13-docosenamide, were discovered and identified. They all indicated the occurrence of benign prostatic hypertrophy is closely related to the disorders of lipid metabolism. Compared with the control group, the contents of the first two substances were significantly increased in the serum of BPH model mice, and significantly decreased after intervened by finasteride. The contents of (Z)-13-docosenamide decreased significantly in the serum of model group, and increased after intervened by finasteride. Compared with the control group, the contents of three biomarkers in finasteride group did not recover completely and had significant differences. This study is conductive to open new avenues of diagnosis and medical treatment for BPH.
1. [1]
  [1] Shin I S, Lee M Y, Lim H S, et al. J Korean Soc Biol Chem, 2012, 55(2): 205
2. [2]
  [2] Wu N, Sun J, Yu P, et al. Int Neurourol J, 2012, 16(4): 191
3. [3]
  [3] Zhang X M, Zhang L H, Zhang Y K. Chinese Journal of Chromatography (张祥民, 张丽华, 张玉奎. 色谱), 2012, 30(3): 222
4. [4]
  [4] Wu H, Liu T, Ma C, et al. Anal Bioanal Chem, 2011, 401(2): 635
5. [5]
  [5] Saylor P J, Karoly E D, Smith M R. Clin Cancer Res, 2012, 18(13): 3677
6. [6]
  [6] Thysell E, Surowiec I, Hörnberg E, et al. PLoS One, 2010, 5(12): e14175
7. [7]
  [7] Bijlsma S, Bobeldijk I, Verheij E R, et al. Anal Chem, 2006, 78(2): 567
8. [8]
  [8] Li L, Luo G A, Lu C J, et al. Journal of Modern Oncology (黎莉, 罗国安, 卢传坚, 等. 现代肿瘤医学), 2011, 19(12): 2361
9. [9]
  [9] Jia D S, Hu W Y. Journal of Clinical Transfusion and Laboratory Medicine (贾道山, 胡文英. 临床输血与检验), 2003, 5(4): 312
10. [10]
  [10] Murphy A B, Macejko A. Drugs, 2009, 69(1): 71
11. [11]
  [11] Yuan Q, Zhang X H, Qiao Y J. China Journal of Traditional Chinese Medicine and Pharmacy (袁奇, 张小华, 乔延江. 中华中医药杂志), 2011, 26(7): 1568
12. [12]
  [12] Bartsch G, Rittmaster R S, Klocker H. Der Urologe Ausg A, 2002, 41(5): 412
13. [13]
  [13] Hai Q M, Shen D L, Liu Z L, et al. Henan Medical Research (海祺旻, 沈德良, 刘琮琳, 等. 河南医学研究), 2014, 23(1): 19
14. [14]
  [14] Jiang L. Journal of Community Medicine (蒋黎. 社区医学杂志), 2014(4): 16
15. [15]
  [15] Li Z W, Wang Z J, Xu X, et al. Journal of Clinical Emergency Call (李志伟, 王著军, 徐旭, 等. 临床急诊杂志), 2013(8): 363
16. [16]
  [16] Sakr M, Eid A, Shoukry M, et al. Bju Int, 2008, 101(3): 2
17. [17]
  [17] Geppetti P, Nassini R, Materazzi S, et al. Bju Int, 2008, 101(s3): 2
18. [18]
  [18] Ackerstaff E, Pflug B R, Nelson J B, et al. Cancer Res, 2001, 61(9): 3599
19. [19]
  [19] Glunde K, Jie C, Bhujwalla Z M. Cancer Res, 2004, 64(12): 4270
20. [20]
  [20] Mills G B, Moolenaar W H. Nat Rev Cancer, 2003, 3(8): 582
21. [21]
  [21] Zhao Z, Xiao Y, Elson P, et al. J Clin Oncol, 2007, 25(19): 2696
22. [22]
  [22] Kang A C, Huo Y, Qi L T. Chinese Journal of Arteriosclerosis (亢爱春, 霍勇, 齐丽彤. 中国动脉硬化杂志), 2006, 14(12): 1083
23. [23]
  [23] Liu J F, Deng M, Hou Y N. Medical Journal of National Defending Forces in North China (刘建芳, 邓鸣, 侯艳宁. 华北国防医药), 2006, 18(4): 286
24. [24]
  [24] Lan R Z, Hu Z Q, Zhuang Q Y. Acta Medicinae Universitatis Scientiae et Technologiae Huazhong (蓝儒竹, 胡志全, 庄乾元. 华中科技大学学报: 医学版), 2009, 38(5): 703
25. [25]
  [25] Hamberger A, Stenhagen G. Neurochem Res, 2003, 28(2): 177
26. [26]
  [26] Xin Z X, Wang D W, Sheng C, et al. Progress in Modern Biomedicine (忻志祥, 王大伟, 盛畅, 等. 现代生物医学进展), 2012, 12(18): 3538
27. [27]
  [27] Hieble J P. Drug Discov Today, 2004, 1(2): 243
28. [28]
  [28] Zhang J S, Liu J L, Liu N, et al. Beijing Medical Journal (张进生, 柳建垒, 刘宁, 等. 北京医学), 2014, 36(4): 292
29. [29]
  [29] Liu Q B. Journal of Qiqihar Medical College (刘巧斌. 齐齐哈尔医学院学报), 2013, 33(22): 3041
1. [1]
  Qian Wu , Yuanxia Lv , Zixuan Guo , Zhihao Zhao , Zhimin Zhang , Hongmei Lu . A Case Study and Practice of Research-Oriented Comprehensive Instrumental Analysis Laboratory Courses. University Chemistry, 2025, 40(10): 194-202. doi: 10.12461/PKU.DXHX202411063
2. [2]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
3. [3]
  Yujing Chen , Hongqun Ouyang , Dan Zhao , Yanyan Chu , Zhengping Qiao . Recommendations for the Content and Instruction of the Physical Chemistry Experiment “Construction of Ternary Liquid-Liquid Phase Diagrams”. University Chemistry, 2025, 40(7): 359-366. doi: 10.12461/PKU.DXHX202409120
4. [4]
  Houjin Li , Lin Wu , Xingwen Sun , Yuan Zheng , Zhanxiang Liu , Shuanglian Cai , Ying Xiong , Guangao Yu , Qingwen Liu , Jie Han , Xin Du , Chengshan Yuan , Qihan Zhang , Jianrong Zhang , Shuyong Zhang . Basic Operations and Specification Suggestions for Organic Chemical Chromatography Experiments. University Chemistry, 2025, 40(5): 93-105. doi: 10.12461/PKU.DXHX202408100
5. [5]
  Yue-Zhou Zhu , Kun Wang , Shi-Sheng Zheng , Hong-Jia Wang , Jin-Chao Dong , Jian-Feng Li . Application and Development of Electrochemical Spectroscopy Methods. Acta Physico-Chimica Sinica, 2024, 40(3): 2304040-0. doi: 10.3866/PKU.WHXB202304040
6. [6]
  Siwei Hou , Yaxin Niu , Guanglu Zhang , Yanmei Yang , Xu Wang , Zhenzhen Chen . Application of Solid-Phase Microextraction and Mass Spectrometry in Environmental Detection. University Chemistry, 2026, 41(3): 297-306. doi: 10.12461/PKU.DXHX202504078
7. [7]
  Xiaoqi LAN , Wei LI , Long JING , Mengyu SU , Xiaoling LUO , Zheng LIU , Qun TANG . Synthesis, crystal structure, and spectral properties of transition-metal-organic frameworks based on thiophene carboxylic acid ligands. Chinese Journal of Inorganic Chemistry, 2026, 42(2): 309-316. doi: 10.11862/CJIC.20250212
8. [8]
  Haiyuan Wang , Xiaoning Jin , Yajing Sun , Zhen Zhang , Wentao Zhao , Yi Li . Practical Exploration of High-Performance Liquid Chromatography Experiment Teaching Reform Empowered by Artificial Intelligence. University Chemistry, 2026, 41(4): 45-51. doi: 10.12461/PKU.DXHX202505076
9. [9]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
10. [10]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
11. [11]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . Sulfide Solid Electrolyte Synthesized by Liquid Phase Approach and Application in All-Solid-State Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(1): 100004-0. doi: 10.3866/PKU.WHXB202309019
12. [12]
  Jia Huo , Jia Li , Yongjun Li , Yuzhi Wang . Ideological and Political Design of Physical Chemistry Teaching: Chemical Potential of Any Component in an Ideal-Dilute Solution. University Chemistry, 2024, 39(2): 14-20. doi: 10.3866/PKU.DXHX202307075
13. [13]
  Jin Tong , Shuyan Yu . Crystal Engineering for Supramolecular Chirality. University Chemistry, 2024, 39(3): 86-93. doi: 10.3866/PKU.DXHX202308113
14. [14]
  Xianfei Chen , Wentao Zhang , Haiying Du . Experimental Design of Computational Materials Science Based on Scientific Research Cases. University Chemistry, 2025, 40(3): 52-61. doi: 10.3866/PKU.DXHX202403112
15. [15]
  Meijin Li , Xirong Fu , Xue Zheng , Yuhan Liu , Bao Li . The Marvel of NAD⁺: Nicotinamide Adenine Dinucleotide. University Chemistry, 2024, 39(9): 35-39. doi: 10.12461/PKU.DXHX202401027
16. [16]
  Jiandong Liu , Zhijia Zhang , Kamenskii Mikhail , Volkov Filipp , Eliseeva Svetlana , Jianmin Ma . Research Progress on Cathode Electrolyte Interphase in High-Voltage Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(2): 100011-0. doi: 10.3866/PKU.WHXB202308048
17. [17]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
18. [18]
  Hongwei Ma , Fang Zhang , Hui Ai , Niu Zhang , Shaochun Peng , Hui Li . Integrated Crystallographic Teaching with X-ray,TEM and STM. University Chemistry, 2024, 39(3): 5-17. doi: 10.3866/PKU.DXHX202308107
19. [19]
  Jiantao Zai , Hongjin Chen , Xiao Wei , Li Zhang , Li Ma , Xuefeng Qian . The Learning-Centered Problem-Oriented Experimental Teaching. University Chemistry, 2024, 39(4): 40-47. doi: 10.3866/PKU.DXHX202309023
20. [20]
  Yang Liu , Peng Chen , Lei Liu . Chemistry “101 Plan”: Design and Construction of Chemical Biology Textbook. University Chemistry, 2024, 39(10): 45-51. doi: 10.12461/PKU.DXHX202407085

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(559)
HTML views(19)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142