Citation: GENG Lijuan, HUANG Junrong, FENG Feng, JIANG Pingping, CHU Xiaogang, ZHANG Feng, LING Yun. Determination of fructooligosaccharides in milk powder using high performance anion-exchange chromatography coupled with pulsed amperometric detection[J]. Chinese Journal of Chromatography, ;2014, 32(12): 1380-1384. doi: 10.3724/SP.J.1123.2014.07052 shu

Determination of fructooligosaccharides in milk powder using high performance anion-exchange chromatography coupled with pulsed amperometric detection

1.
1. College of Life Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China;

Corresponding author: HUANG Junrong, ZHANG Feng,
Received Date: 31 July 2014
Available Online: 25 August 2014
Fund Project: 质检总局公益性行业科研专项项目(2012104002) (2012104002)质检总局科技计划项目(2013IK198) (2013IK198)国家软科学研究计划项目(2010GXS5B153). (2010GXS5B153)

Fructooligosaccharides (FOS) are usually added in milk powder as a kind of prebiotic. Thus, quantitative analysis of the FOS is very important for the quality control of milk powder. In this study, a simple method for the simultaneous determination of three FOS components with degrees of polymerization (DP) 3-5 in milk powder was developed by high performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD). The samples were extracted with 50%(v/v) ethanol aqueous solution and defatted by an On Guard RP pretreatment column. The separation was performed on a CarboPac PA200 column by gradient elution using deionized water, 0.2 mol/L NaOH solution and 0.4 mol/L NaAc solution as the mobile phases. The flow rate was 0.4 mL/min. The column temperature was 30 ℃; and the injection volume was 25 μL. Good linear response was observed in the concentration range of 0.05-10 mg/L (r²>0.9993). The limits of quantification were 0.02, 0.005 and 0.02 mg/L for 1-kestose, nystose and fructofuranosyl-nystose, respectively. The mean recoveries varied from 86.0% to 114.0% at three spiked levels of 0.5, 1.0 and 5.0 mg/L. The short-chain fructooligosaccharides from inulooligosaccharides (IOS) were successfully separated by the developed HPAEC-PAD method. The method is simple, accurate, sensitive, and helpful for the quality control of milk powder.
- high performance anion-exchange chromatography (HPAEC),
- pulsed amperometric detection (PAD),
- fructooligosaccharides (FOS),
- milk powder
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