Citation: HE Dechun, ZHAO Bo, TANG Caiming, XU Zhencheng, ZHANG Sukun, HAN Jinglei. Determination of aniline in water and fish by liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(9): 926-929. doi: 10.3724/SP.J.1123.2014.05025 shu

Determination of aniline in water and fish by liquid chromatography-tandem mass spectrometry

1.
1. South China Institute of Environmental Sciences, Ministry of Environmental Protection, Guangzhou 510655, China;

Corresponding author: XU Zhencheng,
Received Date: 22 May 2014
Available Online: 28 July 2014
Fund Project: 环境保护部公益基金项目(201309001-04) (201309001-04)全国重点地区环境与健康专项调查(21111011101). (21111011101)

A fast analytical method for the determination of aniline in water and fish meat by liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed. The water sample was mixed with acetonitrile by 4:1 (v/v) and the fish sample was extracted by 2.00 mL acetonitrile for each gram of sample, and then the extracts of water and fish samples were centrifuged at 5000 r/min for 5 min. The separation was performed on a reversed-phase C_l8 column using mobile phases of acetonitrile-0.5%(v/v) formic acid aqueous solution (85:15, v/v). Aniline was separated within 3 min. The calibration curve was linear in the range of 0.5-500 μg/L with R²>0.999. The limits of detection (LODs) were 0.50 μg/L and 1.00 μg/kg and the limits of quantification (LOQs) were 1.00 μg/L and 2.00 μg/kg for aniline in water and fish meat, respectively. The average recoveries of aniline in water were 93.7% at the spiked level of 40 ng and 86.7% at the spiked level of 400 ng (n=5). The average recoveries of aniline in fish were 96.8%, 92.6% and 81.8% at the spiked levels of 5, 50 and 500 ng respectively (n=5). The relative standard deviations were 1.5%-9.2%. Thirteen water samples and twelve fish samples were collected from a reservoir polluted by aniline and the maximum contents found were 1943.6 μg/L in water and 60.8 μg/kg in fish. The method is suitable for the determination of aniline residues in water and fish with the characteristics of easy operation, high accuracy and precision.
- liquid chromatography-tandem mass spectrometry (LC-MS/MS),
- aniline,
- water,
- fish
1. [1]
  [1] Zhou W M, Fu D Q, Sun Z G, et al. Chinese Environmental Monitoring (周文敏, 傅德黔, 孙宗光, 等. 中国环境监测), 1990, 6(4): 1
2. [2]
  [2] [2014-05-22]. http://www.cenews.com.cn/kp/hbxbk/201311/t20131114_750084_1.html
3. [3]
  [3] Chen X L, Li Z B, Zhu Y X. et al. Anal Chim Acta, 2004, 505(2): 283
4. [4]
  [4] Zhu Y, Zhu Y, Wang L L. Chinese Journal of Chromatography (诸寅, 朱岩, 王丽丽. 色谱), 2012, 30(4): 345
5. [5]
  [5] Wang W, Huang X H, Wang H. Chinese Journal of Chromatography (王伟, 黄显会, 王辉, 等. 色谱), 2013, 31(10): 1028
6. [6]
  [6] Huang Y, Rao Z, Liu Y, et al. Rock and Mineral Analysis (黄毅, 饶竹, 刘艳, 等. 岩矿测试), 2012, 31(4): 666
7. [7]
  [7] Wang C, Lü Y B, Teng E J, et al. Journal of Instrumental Analysis (王超, 吕怡兵, 滕恩江, 等. 分析测试学报), 2013, 32(1): 32
8. [8]
  [8] Xue K S, Dong F X. Chinese Journal of Analysis Laboratory (薛科社, 董发昕. 分析试验室), 2004, 23(8): 36
9. [9]
  [9] Gu H D, Yin Y M, Qin H B. Environmental Monitoring and Forewarning (顾海东, 尹燕敏, 秦宏兵. 环境监控与预警), 2012, 4(1): 24
10. [10]
  [10] Zou Y L, Chen Y, Lü G B, et al. Chemical Research and Application (邹玉林, 陈勇, 吕桂宾, 等. 化学研究与应用), 2011, 23(5): 649
11. [11]
  [11] Xiong J, Qian S, Xie Y H, et al. Chinese Journal of Analytical Chemistry (熊杰, 钱蜀, 谢永洪, 等. 分析化学), 2014, 42(1): 93
12. [12]
  [12] Farajzadeh M A, Nouri N. Talanta, 2012, 99: 1004
13. [13]
  [13] Schmidt T C, Less M, Haas R, et al. J Chromatogr A, 1998, 810: 161
14. [14]
  [14] Chiang J S, Huang S D. Talanta, 2008, 75: 70
15. [15]
  [15] Chen M, Yin Y G, Tai C, et al. Chinese Science Bulletin (陈明, 阴永光, 邰超, 等. 科学通报), 2006, 51(11): 1359
16. [16]
  [16] Okumura T, Imamura K, Nishikawa Y. J Chromatogr Sci, 1996, 34: 190
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沈阳化工大学材料科学与工程学院沈阳 110142