Citation: SHI Yintao, GUO Jingqi, WANG Huijun, DUAN Jie, YU Hongjiang, ZHENG Jing, WANG Junwei. Rapid screening and confirmation of antidepressants in blood using automated solid phase extraction and liquid chromatography-time-of-flight mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(7): 687-692. doi: 10.3724/SP.J.1123.2014.04013 shu

Rapid screening and confirmation of antidepressants in blood using automated solid phase extraction and liquid chromatography-time-of-flight mass spectrometry

1.
1. Chongqing Institute of Forensic Science, Chongqing 400021, China;

Corresponding author: WANG Junwei,
Received Date: 8 April 2014
Available Online: 7 May 2014
Fund Project: 重庆市公安局科技攻关计划项目(G2013-04). (G2013-04)

A high-throughput method was developed for screening antidepressants in blood by automated solid phase extraction and liquid chromatography with high resolution quadrupole-time-of-flight mass spectrometry (ASPE-LC-Q-TOF/MS). The samples were cleaned up by an HLB solid phase extraction cartridge and analyzed by LC-Q-TOF/MS under electrospray ionization (ESI) mode with scanning range of m/z 50-1000 Da. The chromatographic separation was performed on an Agilent Eclipse Plus C₁₈ column (50 mm×2.1 mm, 1.8 μm) with gradient elution using methanol and 5 mmol/L ammonium formate aqueous solution (containing 0.2% formic acid) as mobile phases. Rapid screening and confirmation can be achieved using MS matching scores, deviation of retention time, measured mass, isotopic abundance matching scores, isotope space matching scores and MS/MS matching scores. The quantitative analysis was carried out by correlating the extracting peak area with accurate mass. Good linearities were observed in the range of 1-500 μg/L with the correlation coefficients from 0.9976 to 0.9997. The limits of detection were 0.01-0.5 μg/L. The spiked recoveries were 79.6%-96.4% with the relative standard deviations of 4.1%-6.4%. The result screening database was built using Agilent MassHunter PCDL Manager software and then used for the analysis of spiked samples. MS matching scores, isotopic abundance matching scores, isotope space matching scores (all > 95 points) and MS/MS matching scores (> 70 points) were applied to identify the analytes. The results showed that all the spiked antidepressants could be correctly identified with low deviation of retention time (< 0.1 min) and mass (< 1 mDa). The developed method was further applied for the analysis of poisoning cases, and amitriptyline, carbamazepine, doxepin were detected. In brief, the method is rapid, sensitive, simple, reliable, and suitable for the screening and confirmation of antidepressants in forensic and clinical analytical toxicology.
- automated solid phase extraction (ASPE),
- liquid chromatography (LC),
- time-of-flight mass spectrometry (TOF/MS),
- antidepressants,
- blood,
- screening,
- confirmation
1. [1]
  [1] Bo N, Yue C H, Sun F C. Medicine & Philosophy (柏宁, 岳长红, 孙福川. 医学与哲学), 2007, 28(3): 26
2. [2]
  [2] Huang F. [PhD Dissertation]. Shanghai: Fudan University (黄菲. [博士学位论文]. 上海: 复旦大学), 2007
3. [3]
  [3] Zheng S Q, Wang W, Liang C, et al. Journal of Forensic Medicine (郑水庆, 王威, 梁晨, 等. 法医学杂志), 2011, 27(4): 271
4. [4]
  [4] Shu C X, Zhang L P, Dong Y, et al. Chinese Journal of Forensic Medicine (舒翠霞, 张蕾萍, 董颖, 等. 中国法医学杂志), 2013, 28(1): 46
5. [5]
  [5] Liao L C, Yan Y Y, Yang L. Journal of Law and Medicine (廖林川, 颜有仪, 杨林. 法律与医学杂志), 2004, 11(2): 131
6. [6]
  [6] Min S, Ping X, He J W, et al. Forensic Sci Int, 2002, 130(2/3): 153
7. [7]
  [7] Zhao X, Liang W B. Chinese Journal of Forensic Medicine (赵霞, 梁武斌. 中国法医学杂志), 2012, 27(3): 222
8. [8]
  [8] Wu H Q, Jin Y C, Cai M Z, et al. Chinese Journal of Analytical Chemistry (吴惠勤, 金永春, 蔡明招, 等. 分析化学), 2007, 35(4): 500
9. [9]
  [9] Ioannis P, Alaa K, Panagiota N, et al. J Pharm Biomed Anal, 2012, 70: 557
10. [10]
  [10] Vardakou I, Dona A, Pistos C, et al. J Chromatogr B, 2010, 878: 2327
11. [11]
  [11] Shi Y T, Zhang S, Ren F, et al. Journal of Instrumental Analysis (石银涛, 张松, 任飞, 等. 分析测试学报), 2012, 31(12): 1595
12. [12]
  [12] Niederlander H A G, Koster E H M, Hilhorst M J, et al. J Chromatogr B, 2006, 834(1/2): 98
13. [13]
  [13] Rooyen G F, Badenhorst D, Swart K J, et al. J Chromatogr B, 2002, 769(1): 1
14. [14]
  [14] Kuchekar S R, Kundlik M L, Zaware B H. J Saudi Chem Soc, 2011, 15: 145
15. [15]
  [15] Danielle S F, Suzanne J P, Simon A H, et al. Forensic Sci Int, 2013, 229(1): 145
16. [16]
  [16] Yuan S L, Li X F, Jiang X M, et al. Chinese Journal of Analytical Chemistry (袁圣柳, 李晓峰, 姜晓满, 等. 分析化学), 2013, 41(1): 49
17. [17]
  [17] Guo J Q, Shi Y T, Wang H J, et al. Chinese Journal of Analytical Chemistry (郭璟琦, 石银涛, 王绘军, 等. 分析化学), 2014, 42(5): 701
18. [18]
  [18] Yan L J, Zhang J, Pan C S, et al. Chinese Journal of Analytical Chemistry (严丽娟, 张洁, 潘晨松, 等. 分析化学), 2013, 41(1): 31
19. [19]
  [19] Liu L H, Bai X, Xiao X Y, et al. Chinese Journal of Pharmaceutical Analysis (刘兰花, 白旭, 肖新月, 等. 药物分析杂志), 2012, 32(6): 950
20. [20]
  [20] Zhang J, Yan L J, Pan C S, et al. Chinese Journal of Chromatography (张洁, 严丽娟, 潘晨松, 等. 色谱), 2012, 30(10): 1031
21. [21]
  [21] Xu J L, Chen D Y, Yan X J, et al. Chinese Journal of Analytical Chemistry (徐继林, 陈德莹, 严小军, 等. 分析化学), 2009, 37(4): 511
22. [22]
  [22] Sebastian B, Sieglinde H, Bernhard W, et al. Anal Bioanal Chem, 2011, 400: 101
23. [23]
  [23] Wilkins C L. Trend Anal Chem, 2007, 26(1): 65
24. [24]
  [24] Zhao Z Y, Shi Z H, Kang J, et al. Chinese Journal of Chromatography (赵志远, 石志红, 康健, 等. 色谱), 2013, 31(4): 372
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