Citation: QIU Zhongli, LIN Ying, XIONG Zhili, XIE Jianwei. Determination of endogenous agmatine in rat plasma by isotope dilution-gas chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(7): 779-783. doi: 10.3724/SP.J.1123.2014.03030 shu

Determination of endogenous agmatine in rat plasma by isotope dilution-gas chromatography-mass spectrometry

QIU Zhongli ^1,2 ,
LIN Ying ¹ ,
XIONG Zhili ^2,, ,
XIE Jianwei ^1,,

1.
1. State Key Laboratory of Toxicology and Medical Countermeasures, and Laboratory of Toxicant Analysis, Institute of Pharmacology and Toxicology, Academy of Military Medical Science, Beijing 100850, China;

Corresponding author: XIONG Zhili, XIE Jianwei,
Received Date: 19 March 2014
Available Online: 7 May 2014

A method for the determination of endogenous agmatine in rat plasma was developed by isotope dilution-gas chromatography-negative chemical ionization mass spectrometry (GC-NCI/MS). The plasma samples were analyzed after protein precipitation, evaporation, derivatization by hexafluoroacetone (HFAA), and clean-up on a Florisil SPE column. The GC-MS analysis utilized stable isotope d₈-agmatine as internal standard. The samples after treatment were tested by negative chemical ionization with selected ion monitoring (SIM) which was set at m/z 492 (molecular ion of agmatine) and m/z 500 (molecular ion of internal standard). The limit of detection (LOD) of agmatine standard solution was 0.0057 ng/mL. The calibration curve of the agmatine spiked in rat plasma showed a good linear relationship at the range of 1.14-57.0 ng/mL (r=0.997). The recoveries of agmatine spiked in rat plasma ranged from 92.3% to 109.8%. Inter-day and intra-day precisions were less than 15%. The average concentration level of agmatine in rat plasma was (22±9) ng/mL, and there was no significant difference between male and female SD rats (p>0.05). The method is high sensitive and specific, and can be used for the determination of endogenous agmatine in plasma. It provides a strong support for the subsequent research of agmatine.
- gas chromatography-negative chemical ionization mass spectrometry (GC-NCI/MS),
- isotope-dilution,
- endogenous agmatine,
- plasma,
- rat
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