Citation: XING Qianqian, FU Qing, JIN Yu, LIANG Xinmiao. Preparation of triterpene saponins from Platycodon grandiflorum by two-dimensional hydrophilic interaction liquid chromatography-reversed phase liquid chromatography[J]. Chinese Journal of Chromatography, ;2014, 32(7): 767-772. doi: 10.3724/SP.J.1123.2014.03027 shu

Preparation of triterpene saponins from Platycodon grandiflorum by two-dimensional hydrophilic interaction liquid chromatography-reversed phase liquid chromatography

1.
School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China

Corresponding author: JIN Yu,
Received Date: 19 March 2014
Available Online: 28 April 2014
Fund Project: 上海市自然科学基金项目(13ZR1453200). (13ZR1453200)

A two-dimensional (2-D) preparative liquid chromatography method was developed for the preparation of triterpene saponins from Platycodon grandiflorum using hydrophilic interaction liquid chromatography (HILIC) coupled with reversed phase liquid chromatography (RPLC). At first, the crude extract was obtained from Platycodon grandiflorum by boiled alcohol precipitation. Then, the concentrated crude extracts were continuously pretreated using solid phase extraction (SPE) under reversed-phase and hydrophilic-phase modes to remove the impurities. Subsequently, XAmide column (150 mm×20 mm, 5 μm) was selected to separate the triterpene saponin constituents under HILIC mode using water and acetonitrile as mobile phases. From 6 min to 25 min, each fraction was collected per minute under time-triggered mode and 20 fractions were collected. The 18th fraction (JG23) was selected for further purification. The column of Atlantis PrepT3 (100 mm×30 mm, 5 μm) was chosen and two monomeric compounds were obtained. The two compounds with over 90% purity were identified as deapi-platycoside E and platycoside E with mass spectrometry (MS) and nuclear magnetic resonance (NMR). This 2-D HILIC-RPLC method with high orthogonality can be used in the preparation of triterpene saponins from natural products.
1. [1]
  [1] Zhao X L. China Condiment (赵秀玲. 中国调味品), 2012, 37(2): 5
2. [2]
  [2] He Z D, Qiao C F, Han Q B, et al. Tetrahedron, 2005, 61: 2211
3. [3]
  [3] He M L, Cheng X W, Chen J K, et al. Traditional Chinese Medicine New Drug and Clinical Pharmacology (何美莲, 程小卫, 陈家宽, 等. 中药新药与临床药理), 2005, 16(6): 457
4. [4]
  [4] Young W H, Yeong S K. Phytochem Anal, 2009, 20: 207
5. [5]
  [5] In J H, Minseok K, Yun C N, et al. J Sep Sci, 2011, 34: 2559
6. [6]
  [6] Zhu F X, Jia X B, Cai Y, et al. Acta Chromatographica, 2013, 25(1): 147
7. [7]
  [7] Sun B S, Gu L J, Fang Z M, et al. J Pharm Biomed Anal, 2009, 50: 15
8. [8]
  [8] Zhang J, Wang L L, Shan L G, et al. Chinese Journal of Chromatography (张静, 王玲玲, 单联国, 等. 色谱), 2012, 30(8): 804
9. [9]
  [9] Shi Y M, Yang H, Li Y Y, et al. Food Research and Development (史一鸣, 杨虹, 李燕艳, 等. 食品研究与开发), 2012, 33(12): 134
10. [10]
  [10] Noel S Q, Nerissa L D, Azamjon B S, et al. Anal Bioanal Chem, 2007, 389: 1477
11. [11]
  [11] Xing Q Q, Liang T, Shen G B, et al. Analyst, 2012, 137: 2239
12. [12]
  [12] Boguslaw B, Sylwia N. Anal Bioanal Chem, 2012, 402: 231
13. [13]
  [13] Li W, Zhang W, Xiang L, et al. Molecules, 2010, 15: 8702
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