Citation: HUANG Hehe, ZHANG Jin, XU Dunming, ZHOU Yu, LUO Jia, LV Meiling, CHEN Shubin, WANG Lianzhu. Determination of 21 plant growth regulator residues in fruits by QuEChERS-high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(7): 707-716. doi: 10.3724/SP.J.1123.2014.03003 shu

Determination of 21 plant growth regulator residues in fruits by QuEChERS-high performance liquid chromatography-tandem mass spectrometry

HUANG Hehe ^1,2 ,
ZHANG Jin ¹ ,
XU Dunming ^1,, ,
ZHOU Yu ¹ ,
LUO Jia ² ,
LV Meiling ³ ,
CHEN Shubin ⁴ ,
WANG Lianzhu ¹

1.
1. Xiamen Entry-Exit Inspection and Quarantine Bureau, Xiamen 361026, China;

Corresponding author: XU Dunming,
Received Date: 5 March 2014
Available Online: 17 April 2014
Fund Project: 福建省自然科学基金项目(2013J01031) (2013J01031)浙江省公益性技术应用研究计划项目(2012C37074). (2012C37074)

A method for the simultaneous detection of 21 plant growth regulators in fruits by QuEChERS-high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed. The samples were initially extracted with acetonitrile containing 1%(v/v) acetic acid, followed by clean-up using the powder of magnesium sulfate and C18. The resulting samples were separated on a C18 column, and detected under positive and negative multiple reactions monitoring (MRM) mode through polarity switching between time segments. The matrix-matched external standard calibration curves were used for quantitative analysis. The linearities of chlormequat chloride, mepiquat chloride, choline chloride, cyclanilide, forchlorfenuron, thidiazuron, inabenfide, paclobutrazol, uniconazole and triapenthenol were in the concentration range of 0.1-500 μg/L, daminozide and 6-benzylaminopurine in the concentration range of 1.0-500 μg/L, 2,3,5-triiodobenzoic acid, 2,4-D, cloprop, 4-chlorophenoxyacetic acid (4-CPA) and trinexapac-ethyl in the concentration range of 2.0-1000 μg/L, abscisic acid (ABA), gibberellic acid (GA3), 1-naphthaleneacetic acid (NAA) and indol-3-ylacetic acid (IAA) in the concentration range of 10-1000 μg/L, with the correlation coefficients higher than 0.990. The limits of detection and the limits of quantification of the method were 0.020-6.0 μg/kg and 0.10-15.0 μg/kg, respectively. For all the samples, the average spiked recoveries ranged from 73.0% to 111.0%, and the relative standard deviations (RSDs, n=6) were in the range of 3.0%-17.2%. The method is quick, easy, effective, sensitive and accurate, and can meet the requirements for the determination of the 21 plant growth regulator residues in fruits.
- QuEChERS,
- high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS),
- plant growth regulators,
- fruits
1. [1]
  [1] Giannakoula A E, Ilias I F, Dragisic Maksimovic J J, et al. J Food Compos Anal, 2012, 28(1): 46
2. [2]
  [2] Li L, Tian S L. Journal of Anhui Agricutural Science (李莉, 田士林. 安徽农业科学), 2007, 35(23): 7098
3. [3]
  [3] Wu S R, Chen W F, Zhou X. Plant Physiology Communications (吴颂如, 陈婉芬, 周燮. 植物生理学通讯), 1988(5): 53
4. [4]
  [4] Santos-Delgado M J, Crespo-Corral E, Polo-Diez L M. Talanta, 2000, 53(2): 367
5. [5]
  [5] Zhou Y M, Fu T, Hu R. Food Science (周艳明, 付婷, 胡睿. 食品科学), 2010, 31(12): 178
6. [6]
  [6] Jia J, Wang X Y, Yang T Z. Chemical Engineer (贾建, 王晓云, 杨天祝. 化学工程师), 2004, 18(7): 57
7. [7]
  [7] Song Y, Zhang Y H, Huang X, et al. Chinese Journal of Analytical Chemistry (宋莹, 张耀海, 黄霞, 等. 分析化学), 2011, 39(8): 1270
8. [8]
  [8] Xu Y H, Jiang S, Fu H B, et al. Agrochemicals (徐宜宏, 蒋施, 付海滨, 等. 农药), 2014, 53(2): 113
9. [9]
  [9] Zhao Y X, Song G L, Zhang J, et al. Chinese Journal of Health Laboratory Technology (赵永信, 宋国良, 张晶, 等. 中国卫生检验杂志), 2008, 18(6): 1064
10. [10]
  [10] Kuang K, Zhao J L, Ying G G, et al. Journal of Instrumental Analysis (匡科, 赵建亮, 应光国, 等. 分析测试学报), 2008, 27(8): 816
11. [11]
  [11] Lü Y, Zhao J, Yang T, et al. China Measurement and Test (吕燕, 赵健, 杨挺, 等. 中国测试), 2010, 36(4): 57
12. [12]
  [12] Lu Y M, Yi G B, Chen C B, et al. Journal of Instrumental Analysis (陆益民, 易国斌, 陈创彬, 等. 分析测试学报), 2011, 30(2): 186
13. [13]
  [13] Zhang Y, Lu Y, Yang T, et al. Journal of Analytical Science (张莹, 鹿毅, 杨涛, 等. 分析科学学报), 2012, 28(5): 629
14. [14]
  [14] Zhou J K, Xu P, Yang D X, et al. China Condiment (周建科, 徐鹏, 杨冬霞, 等. 中国调味品), 2011, 36(3): 99
15. [15]
  [15] Dong S Y, Hu Q, Li J, et al. Chinese Journal of Analysis Laboratory (董社英, 胡清, 李靖, 等. 分析试验室), 2013, 32(8): 48
16. [16]
  [16] Mol H G J, Van Dam R C J, Vreeken R J, et al. J Chromatogr A, 1999, 833(1): 53
17. [17]
  [17] Esparza X, Moyano E, Galceran M T. J Chromatogr A, 2009, 1216: 4402
18. [18]
  [18] Riediker S, Obrist H, Varga N, et al. J Chromatogr A, 2002, 966(1): 15
19. [19]
  [19] Ma L Y, Zhang H Y, Xu W T, et al. Food Anal Methods, 2013, 6(3): 941
20. [20]
  [20] Mou Y L, Guo D H, Ding Z P. Chinese Journal of Chromatography (牟艳莉, 郭德华, 丁卓平. 色谱), 2013, 31(10): 1016
21. [21]
  [21] Zhang J, Du P. Chinese Journal of Chromatography (张军, 杜平. 色谱), 2011, 29(11): 1133
22. [22]
  [22] Xie W, Shi Y Z, Hou J B, et al. Chinese Journal of Chromatography (谢文, 史颖珠, 侯建波, 等. 色谱), 2014, 32(2): 179
23. [23]
  [23] Zhou X, Xu J G, Chen Z D, et al. Chinese Journal of Chromatography (周旭, 许锦钢, 陈智栋, 等. 色谱), 2011, 29(3): 244
24. [24]
  [24] Huang B Y, Xiao Z Y, Chen D, et al. Pesticide Science and Administration (黄宝勇, 肖志勇, 陈丹, 等. 农药科学与管理), 2010, 31(3): 39
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