Citation: ZHANG Xiuyao, CAI Xinxin, ZHANG Xiaoyi. Determination of α-solanine,α-chaconine and solanidine in plasma and urine by ultra-performance liquid chromatography-triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(6): 586-590. doi: 10.3724/SP.J.1123.2014.03001 shu

Determination of α-solanine,α-chaconine and solanidine in plasma and urine by ultra-performance liquid chromatography-triple quadrupole mass spectrometry

1.
Wenzhou Center for Disease Control and Prevention, Wenzhou 325001, China

Corresponding author: ZHANG Xiuyao,
Received Date: 3 March 2014
Available Online: 26 March 2014
Fund Project: 温州市医学重点学科、温州市第三轮“311”工程建设项目基金(2008012). (2008012)

An ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS) method was developed for the determination of α-solanine,α-chaconine and solanidine in plasma and urine. The sample was acidified with aqueous solution containing 2% (v/v if not specified) formic acid, and then cleaned-up by solid-phase extraction with a mixed-mode cation exchange (MCX) cartridge. The analysis of the glycoalkaloids was carried out on an Acquity UPLC BEH C₁₈ column (50 mm×2.1 mm, 1.7 μm) with gradient elution of acetonitrile (containing 0.1% formic acid) and H₂O (containing 0.05% formic acid and 5.0 mmol/L ammonium acetate). The analytes were detected by positive electrospray ionization tandem mass spectrometry in MRM mode, and quantified by external matrix-matched standard calibration. The cycle time of each analysis was 5.5 min. The calibration curves were linear in the range of 0.3-100 ng/mL of the glycoalkaloids in plasma and urine. The correlation coefficients were 0.997-0.999. The limits of detection (S/N=3) and quantitation (S/N=10) were 0.1 ng/mL and 0.3 ng/mL. The average recoveries were 82%-112% and 96%-114% for the glycoalkaloids spiked in plasma and urine, respectively, with relative standard deviations of 4.0%-16% and 2.7%-17% (n=6). The method is simple, accurate and sensitive to detect the glycoalkaloids in plasma and urine for both clinical and forensic purposes.
- ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS),
- tandem mass spectrometry,
- α-solanine,
- α-chaconine,
- solanidine,
- plasma,
- urine,
- potato
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沈阳化工大学材料科学与工程学院沈阳 110142