Citation: ZHANG Wenhua, HUANG Chaoqun, XIE Wen, SHEN Li. Determination of gossypol in edible vegetable oil with high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(6): 582-585. doi: 10.3724/SP.J.1123.2014.02029 shu

Determination of gossypol in edible vegetable oil with high performance liquid chromatography-tandem mass spectrometry

ZHANG Wenhua ¹ ,
HUANG Chaoqun ¹ ,
XIE Wen ^1,, ,
SHEN Li ²

1.
1. Zhejiang Entry-Exit Inspection and Quarantine Bureau, Hangzhou 310016, China;

Corresponding author: XIE Wen,
Received Date: 28 February 2014
Available Online: 4 April 2014
Fund Project: 出入境检验检疫行业标准制(修)订项目(2012B308r). (修)订项目(2012B308r)

A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the determination of gossypol in edible vegetable oil. The sample was extracted with ethyl alcohol by vortex-excited oscillation. The extract was cleaned up by 0.22 μm filter membrane and centrifuged for 5 min at 4 000 r/min after standing in a fridge at 4 ℃ for 30 min. The compound was separated on a C18 column (100 mm×2.1 mm, 3.5 μm) with acetonitrile and 1% (v/v) formic acid aqueous solution as mobile phase. The detection of gossypol was carried out by LC-MS/MS with positive electrospray ionization under multiple reaction monitoring (MRM) mode using external standard method. The limits of quantification (S/N>10) of gossypol in edible vegetable oil was 1 mg/kg. The recoveries were from 87.4% to 100% at the spiked levels of 1, 2, 200 mg/kg of gossypol in edible vegetable oil with the relative standard deviations (RSDs) between 3.9% and 12.2%. The method, with high sensitivity, good precision and high recovery, was suitable for the confirmation and quantification of gossypol residue in edible vegetable oil.
- liquid chromatography-tandem mass spectrometry (LC-MS/MS),
- gossypol,
- edible oil
1. [1]
  [1] Baidu Encyclopedia (百度百科). (2007-05-29)[2014-01-20]. http://baike.baidu.com/link?url=cYITcw-NkA8nhF6RAY6U71ToagC04IxOoCrNqBC8PNzGZb8_AxxPbIAhxPCWlqfh
2. [2]
  [2] GB 2716-2005
3. [3]
  [3] Food Mate Net (食品伙伴网). (2013-10-29)[2014-01-20]. http://news.foodmate.net/2013/10/246990.html
4. [4]
  [4] Yu J Q. Science and Technology of Cereals, Oils and Foods (于加乾. 粮油食品科技), 2013, 21(4): 78
5. [5]
  [5] Zhang Q Z. Guangzhou Chemical Industry (张庆珍. 广州化工), 2012, 40(15): 159
6. [6]
  [6] Wen J, Miao H, Wang X J, et al. Chinese Journal of Health Laboratory Technology (文君, 缪红, 王鲜俊, 等. 中国卫生检验杂志), 2006, 16(8): 1017
7. [7]
  [7] Shen J, Yang S, Yu H, et al. Chinese Journal of Spectroscopy Laboratory (沈静, 阳胜, 于红, 等. 光谱实验室), 2011, 28(1): 235
8. [8]
  [8] Zhao H X. Food Engineering (赵海霞. 食品工程), 2012(4): 47
9. [9]
  [9] Tian X Y, Yi J G, Xu S J, et al. Endemic Diseases Bulletin (田秀芸, 易建国, 徐世江, 等. 地方病通报), 2003, 18(4): 91
10. [10]
  [10] Shi Q Y. Modern Food Science and Technology (施琦贻. 现代食品科技), 2012, 28(5): 598
11. [11]
  [11] Yao J, He X W, Li X X, et al. Science and Technology of Food Industry (姚军, 何晓文, 李新霞, 等. 食品工业科技), (2013-05-02)[2014-01-20]. http://www.cnki.net/kcms/detail/11.1759.TS.20130502.1348.010.html
12. [12]
  [12] Nomeir A A, Abou-Donia M B. J Am Oil Chem Soc, 1982, 59: 546
13. [13]
  [13] Wu W W, Xu G R. Journal of Agricultural University of Hebei (吴伟伟, 许赣荣. 河北农业大学学报), 2009, 32(5): 106
14. [14]
  [14] Shen Y, Xue Y L, Jin Q Z, et al. China Oils and Fats (沈媛, 薛雅琳, 金青哲, 等. 中国油脂), 2012, 37(10): 71
15. [15]
  [15] Ma Y J, Shi G Y, Chen B. Journal of Hygiene Research (马勇健, 石根勇, 陈蓓. 卫生研究), 1993, 22(5): 302
16. [16]
  [16] Xiang P, Shen M, Zhuo X Y. Journal of Instrumental Analysis (向平, 沈敏, 卓先义. 分析测试学报), 2009, 28(6): 753
17. [17]
  [17] Perello A E, Moreno M V, Monaco C, et al. Biocontrol, 2009, 54(1): 113
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