Citation: ZHANG Hongwei, XU Hui, GAO Jianguo, LIANG Chengzhu, XU Biao, GENG Juan, WANG Fengmei, ZHANG Xiaomei, CHENG Gang. Simultaneous determination of nine β-blockers in porcine tissues by ultra-fast liquid chromatography coupled with quadrupole/linear ion trap mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(6): 573-581. doi: 10.3724/SP.J.1123.2014.02014 shu

Simultaneous determination of nine β-blockers in porcine tissues by ultra-fast liquid chromatography coupled with quadrupole/linear ion trap mass spectrometry

1.
1. Technical Center of Inspection and Quarantine, Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, China;

Corresponding author: ZHANG Hongwei,
Received Date: 17 February 2014
Available Online: 21 March 2014
Fund Project: 青岛市公共领域科技支撑计划项目(12-1-3-80-jh) (12-1-3-80-jh)山东出入境检验检疫局科研攻关项目(SK201222). (SK201222)

A highly sensitive method using ultra-fast liquid chromatography coupled with quadrupole/linear ion trap mass spectrometry (UFLC-Q/Trap MS) was developed to simultaneously screen and confirm nine β-blockers (BBs) in porcine tissues (porcine muscle, liver and kidney). The method was used for trace determination of atenolol, pindolol, acebutolol, metoprolol, carazolol, labetalol, bisoprolol, propranolol and penbutolol. The homogenized tissues were hydrolyzed by β-glucuronidase/aryl sulfatase and extracted with acetonitrile, followed by continuous purification procedures of disperse solid phase extraction (d-SPE) with diatomaceous earth and BondElut cartridge. The ultra-fast chromatographic separation was conducted on a Kinetex^TM C18-XB column (150 mm×2.1 mm, 2.6 μm) using 0.1% (v/v) formic acid aqueous solution and methanol as mobile phases in gradient elution. The optimized ion transitions were employed in the mixed-mode of scheduled multiple reaction monitoring (sMRM)-information dependent acquisition (IDA)-enhanced product ion (EPI) scan. Qualification analysis was performed through spectra-matching with on-line lab-built MS/MS library. For quantification stable isotope-labelled analogues of the analytes were used as internal standards. As a result, in porcine liver, kidney and muscle, the nine BBs showed good linearity with all the correlation coefficients (r) more than 0.995 in the range of 0.1-20 μg/L. The limits of quantification (LOQ, S/N≥10) were 0.5 μg/kg for all the analytes. The developed method gave average recoveries of 87.5%-111.8% spiked at 0.5, 1.0 and 5.0 μg/kg with the relative standard deviations of 4.0%-12.5%. The proposed method can be used to screen and confirm the nine BBs in a single run, which makes it effective in surveillance and detection of the BBs residues in porcine tissues.
- ultra-fast liquid chromatography (UFLC),
- quadrupole/linear ion trap mass spectrometry (Q/Trap MS),
- β-blockers (BBs),
- residue,
- porcine tissues
1. [1]
  [1] Sleiman O I, Murin J, Ghanem W M. Bratisl Med J, 2001, 102(7): 332
2. [2]
  [2] Hernando M D, Gomez M J, Aguera A, et al. Trends Anal Chem, 2007, 26(6): 581
3. [3]
  [3] World Anti-Doping Agency. The World Anti-Doping Code: The 2013 Prohibited List, International Standard.[2013-12-26]. http://www.wada-ama.org/en/Science-Medicine/Prohibited-List/
4. [4]
  [4] Balizs G, Hewitt A. Anal Chim Acta, 2003, 492(1): 105
5. [5]
  [5] Pang G F, et al. Modern Techniques for Residue Analysis of Pesticides and Veterinary Drugs. Beijing: Science Press (庞国芳, 等. 农药兽药残留现代分析技术. 北京: 科学出版社), 2007
6. [6]
  [6] CODEX Alimentarius. Veterinary Drug Residues in Food.[2013-12-26]. http://www.codexalimentarius.net/vetdrugs/data/vetdrugs/index.html
7. [7]
  [7] Commission Regulation (EU) 37/2010
8. [8]
  [8] Zhang J, Shao B, Yin J, et al. J Chromatogr B, 2009, 877: 1915
9. [9]
  [9] Fan S, Miao H, Zhao Y F, et al. J Agric Food Chem, 2012, 60(8): 1898
10. [10]
  [10] Mitrowska K, Posyniak A, Zmudzki J. Anal Chim Acta, 2009, 637(1/2): 185
11. [11]
  [11] Anastassiades M, Lehotay S J, Stajnbaher D, et al. J AOAC Int, 2003, 86(2): 412
12. [12]
  [12] Stubbings G, Bigwood T. Anal Chim Acta, 2009, 637(1/2): 68
13. [13]
  [13] Aguilera-Luiz M M, Martinez Vidal J L, Romero-Gonzalez R, et al. Food Chem, 2012, 132(4): 2171
14. [14]
  [14] Lombardo-Agui M, Garcia-Campanna A M, Gamiz-Gracia L, et al. Talanta, 2012, 93: 193
15. [15]
  [15] O'Mahony J, Clarke L, Whelan M, et al. J Chromatogr A, 2013, 1292: 83
16. [16]
  [16] Zhang H W, Jian H M, Lin L M, et al. Journal of Instrumental Analysis (张鸿伟, 简慧敏, 林黎明, 等. 分析测试学报), 2012, 31(7): 763
17. [17]
  [17] Zhang H W, Cai X, Lin L M, et al. Chinese Journal of Chromatography (张鸿伟, 蔡雪, 林黎明, 等. 色谱), 2012, 30(10): 991
18. [18]
  [18] Dahane S, Gil Garcia M D, Martinez Bueno M J, et al. J Chromatogr A, 2013, 1297: 17
19. [19]
  [19] Salem A A, Wasfi I A, Al-Nassibi S S, et al. J Chromatogr B, 2012, 908: 27
20. [20]
  [20] Commission Decision 2002/657/EC
21. [21]
  [21] Kaufmann A, Butcher P, Maden K, et al. Rapid Commun Mass Spectrom, 2009, 23(7): 985
22. [22]
  [22] Chester T L. Anal Chem, 2013, 85(2): 579
23. [23]
  [23] Kaufmann A, Butcher P, Maden K, et al. Anal Chim Acta, 2011, 700(1/2): 86
24. [24]
  [24] Wang J, Xiao T A, Fang B H, et al. Progress in Veterinary Medicine (王军, 肖田安, 方炳虎, 等. 动物医学进展), 2011, 32(3): 113
25. [25]
  [25] Peters R J B, Oosterink J E, Stolker A A M, et al. Anal Bioanal Chem, 2010, 396(7): 2583
26. [26]
  [26] Kumar P, Rubies A, Centrich F, et al. Anal Chim Acta, 2013, 780: 65
27. [27]
  [27] Gomez M J, Petrovic M, Fernandez-Alba A R, et al. J Chromatogr A, 2006, 1114: 224
28. [28]
  [28] Choi J H, Lamshoft M, Sebastian Z, et al. J Chromatogr A, 2012, 1260: 111
29. [29]
  [29] Kadam R S, Kompella U B. J Chromatogr B, 2009, 877: 253
30. [30]
  [30] Vieno N M, Tuhkanen T, Kronberg L. J Chromatogr A, 2006, 1134: 101
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