Citation: PENG Xitian, FENG Yuqi. Preparation and retention mechanism of a mixed-mode reversed-phase/weak-cationic-exchange chromatographic packing[J]. Chinese Journal of Chromatography, ;2014, 32(4): 381-387. doi: 10.3724/SP.J.1123.2013.10038 shu

Preparation and retention mechanism of a mixed-mode reversed-phase/weak-cationic-exchange chromatographic packing

PENG Xitian ^1,2 ,
FENG Yuqi ^1,,

1.
1. Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China;

Corresponding author: FENG Yuqi,
Received Date: 31 October 2013
Available Online: 6 December 2013
Fund Project: 国家自然科学基金项目(91017013)；湖北省农业科学院青年基金(2013NKYJJ18). (91017013)；湖北省农业科学院青年基金(2013NKYJJ18)

A "mixed ligand" octyl-carboxylic co-bonded silica (OCS) packing was prepared by the method of "click chemistry". The resulting OCS packing was characterized by elemental analysis (EI) and Fourier transform infrared spectroscopy (FT-IR) to prove the successful immobilization of octyl and carboxylic groups on the surface of silica gel. Then the mixed-mode reversed-phase/weak-cationic-exchange (RP/WCX) retention mechanism of the OCS packing was quantitatively probed by studying the retention factors of a homologous series of three cationic surfactants on the mixed-mode stationary phase column as a function of the ammonium concentration in the eluent or the number of methylene groups in the solute. The one-site and two-site mixed-mode retention models of the three cationic surfactants on the OCS phases were studied by investigating the logarithm and reciprocal relationships of retention factors and salt concentrations, demonstrating that the two-site model was more suitable for the description of the retention mechanism of the three cationic surfactants on the OCS phases. Furthermore, the individual RP or WCX contribution to total retention was obtained according to the mathematical equations of two-site retention mechanism, which can provide some valuable guidance for the separation of real samples. This study developed the qualitative model of retention mechanism of the mixed-mode OCS packing, and a series of standard basic mixtures were well separated on the OCS packing, demonstrating the great application potential of OCS packing for the separation of various basic compounds.
1. [1]
  [1] Geng X D. Chinese Journal of Chromatography (耿信笃. 色谱), 2012, 30(1): 1
2. [2]
  [2] Dong X F, Cai X M, Shen A J, et al. Chinese Journal of Chromatography (董雪芳, 蔡晓明, 沈爱金, 等. 色谱), 2013, 31 (4): 297
3. [3]
  [3] Yang Y, Geng X D. J Chromatogr A, 2011, 1218(49): 8813
4. [4]
  [4] Liu X, Pohl C. Am Lab, 2009, 41(5): 26
5. [5]
  [5] Cai X M, Guo Z M, Xue X Y, et al. J Chromatogr A, 2012, 1228: 242
6. [6]
  [6] Leicunaite J, Klimenkovs I, Kviesis J, et al. C R Chim, 2011, 13(10): 1335
7. [7]
  [7] Davies N H, Euerby M R, McCalley D V. J Chromatogr A, 2007, 1138(1/2): 65
8. [8]
  [8] Chua H C, Lee H S, Sng M T. J Chromatogr A, 2006, 1102(1/2): 214
9. [9]
  [9] Zhang Y, Carr P W. J Chromatogr A, 2011, 1218(6): 763
10. [10]
  [10] Yang X Q, Dai J, Carr P W. J Chromatogr A, 2003, 996(1/2): 13
11. [11]
  [11] Peng X T, Wang J, Feng Y Q. Chinese Journal of Chromatography (彭西甜, 王珏, 冯钰锜. 色谱), 2013, 31(4): 329
12. [12]
  [12] Peng X T, Li Z, Zhang Y, et al. Chromatographia, 2013, 76(13/14): 735
13. [13]
  [13] Wang Y, Xiao Y, Tan T T Y, et al. Tetrahedron Lett, 2008, 49(35): 5190
14. [14]
  [14] Guo Z M, Lei A W, Liang X M, et al. Chem Commun, 2006(43): 4512
15. [15]
  [15] Slater M D, Frechet J M J, Svec F. J Sep Sci, 2009, 32(1): 21
16. [16]
  [16] Vigh G Y, Varga-Puchony Z. J Chromatogr, 1980, 196(1): 1
1. [1]
  Qiuting Zhang , Fan Wu , Jin Liu , Zian Lin . Chromatographic Stationary Phase and Chiral Separation Using Frame Materials. University Chemistry, 2025, 40(4): 291-298. doi: 10.12461/PKU.DXHX202405174
2. [2]
  Lingyu Chang , Yanfang Lang , Yuyan Zhu , Jie Wang , Ying Guo , Die Wang , Peng Ding , Yueming Zhou , Zhixiang Gong , Shujuan Liu . Machine Learning-Optimized Microcolumn Ion Exchange Chromatography for Trace Arsenic Determination. University Chemistry, 2026, 41(1): 76-84. doi: 10.12461/PKU.DXHX202506023
3. [3]
  Tong Wang , Liangyu Hu , Shiqi Chen , Xinqiang Fu , Rui Wang , Kun Li , Shuangyan Huan . Determination of Benzenediol Isomers in Cosmetics Using High-Performance Liquid Chromatography Empowered by “Mathematical Separation”. University Chemistry, 2026, 41(1): 9-19. doi: 10.12461/PKU.DXHX202503128
4. [4]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
5. [5]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
6. [6]
  Fan Wu , Wenchang Tian , Jin Liu , Qiuting Zhang , YanHui Zhong , Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031
7. [7]
  Houjin Li , Lin Wu , Xingwen Sun , Yuan Zheng , Zhanxiang Liu , Shuanglian Cai , Ying Xiong , Guangao Yu , Qingwen Liu , Jie Han , Xin Du , Chengshan Yuan , Qihan Zhang , Jianrong Zhang , Shuyong Zhang . Basic Operations and Specification Suggestions for Organic Chemical Chromatography Experiments. University Chemistry, 2025, 40(5): 93-105. doi: 10.12461/PKU.DXHX202408100
8. [8]
  Jingming Li , Bowen Ding , Nan Li , Nurgul . Application of Comparative Teaching Method in Experimental Project Design of Instrumental Analysis Course: A Case Study in Chromatography Experiment Teaching. University Chemistry, 2024, 39(8): 263-269. doi: 10.3866/PKU.DXHX202312078
9. [9]
  Haiyuan Wang , Xiaoning Jin , Yajing Sun , Zhen Zhang , Wentao Zhao , Yi Li . Practical Exploration of High-Performance Liquid Chromatography Experiment Teaching Reform Empowered by Artificial Intelligence. University Chemistry, 2026, 41(4): 45-51. doi: 10.12461/PKU.DXHX202505076
10. [10]
  Wenwen Ma , Liyan Liu , Chengyang Yin , Hongdan Zhang , Lian Kong , Na Wei , Zhan Yu , Zhen Zhao . Exploration of the Online and Offline Mixed Teaching Mode of Specialized English for Chemistry Majors Based on the BOPPPS Model. University Chemistry, 2025, 40(9): 287-294. doi: 10.12461/PKU.DXHX202410026
11. [11]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
12. [12]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
13. [13]
  Shunü Peng , Huamin Li , Zhaobin Chen , Yiru Wang . Simultaneous Application of Multiple Quantitative Analysis Methods in Gas Chromatography for the Determination of Active Ingredients in Traditional Chinese Medicine Preparations. University Chemistry, 2025, 40(10): 243-249. doi: 10.12461/PKU.DXHX202412043
14. [14]
  Qijia BAI , Xiang GAO , Yihang SHEN , Jiaqi LI , Yang CHEN , Cuie ZHAO . Mixed-size MXene for the application of high-performance transparent zinc-ion hybrid supercapacitors. Chinese Journal of Inorganic Chemistry, 2026, 42(4): 703-712. doi: 10.11862/CJIC.20250331
15. [15]
  Hongli CHEN , Ziling XU , Shiwen DU , Ting WANG , Liguang WU . Controlled preparation of HKUST-1 based on polyvinylpyrrolidone and CO₂ separation performance of its doped mixed-matrix membranes. Chinese Journal of Inorganic Chemistry, 2026, 42(3): 571-583. doi: 10.11862/CJIC.20250263
16. [16]
  Wendian XIE , Yuehua LONG , Jianyang XIE , Liqun XING , Shixiong SHE , Yan YANG , Zhihao HUANG . Preparation and ion separation performance of oligoether chains enriched covalent organic framework membrane. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1528-1536. doi: 10.11862/CJIC.20240050
17. [17]
  Zhiming Feng , Lili Wu , Chengming Wang . Doubly Oxidized Carbene. University Chemistry, 2025, 40(9): 326-331. doi: 10.12461/PKU.DXHX202411008
18. [18]
  Fangdong Hu , Xiaolei Jiang . Research and Practice of the “Integration of Theory and Practice Drives Innovation” Teaching Mode in Inorganic Chemistry under the Background of “Four New” Construction. University Chemistry, 2024, 39(11): 1-8. doi: 10.3866/PKU.DXHX202402013
19. [19]
  Zhipeng Bao , Yilin Wang , Yu Chen , Beirui Jia , Congcong Wang , Zean Xie , Xuehua Yu , Zhen Zhao . Digital and Intelligent Integration under the “Dual Carbon” Strategy: Plasma Reaction-Separation Coupling for CO₂ Hydrogenation to Methanol. University Chemistry, 2026, 41(1): 29-40. doi: 10.12461/PKU.DXHX202506009
20. [20]
  Wen Shi , Jiuxing Jiang . 化学中的数学方法课程建设探索. University Chemistry, 2025, 40(6): 48-53. doi: 10.12461/PKU.DXHX202408088

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(841)
HTML views(69)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142