生物表面活性剂鼠李糖脂的纯化与表征

刘洋 钟华 刘智峰 蒋勇兵 谈菲 曾光明 赖明勇 何益斌

引用本文: 刘洋, 钟华, 刘智峰, 蒋勇兵, 谈菲, 曾光明, 赖明勇, 何益斌. 生物表面活性剂鼠李糖脂的纯化与表征[J]. 色谱, 2014, 32(3): 248-255. doi: 10.3724/SP.J.1123.2013.10026 shu
Citation:  LIU Yang, ZHONG Hua, LIU Zhifeng, JIANG Yongbing, TAN Fei, ZENG Guangming, LAI Mingyong, HE Yibin. Purification and characterization of the biosurfactant rhamnolipid[J]. Chinese Journal of Chromatography, 2014, 32(3): 248-255. doi: 10.3724/SP.J.1123.2013.10026 shu

生物表面活性剂鼠李糖脂的纯化与表征

    通讯作者: 曾光明, 钟华; 曾光明, 钟华
  • 基金项目:

    国家自然科学基金项目(51378190,50908081,51039001,51378192,51308200). (51378190,50908081,51039001,51378192,51308200)

摘要: 生物表面活性剂鼠李糖脂是微生物在一定条件下产生的次级代谢产物,其分子具有极性亲水基团和非极性亲油基团结构,通常表现出很高的表面活性和界面优先分配能力。可靠的分离提纯方法和成分鉴定手段是鼠李糖脂生产工艺成功的重要保证。实验通过好氧发酵培养铜绿假单胞菌CCTCC AB93066、酸沉降分离得到鼠李糖脂后,利用柱色谱提纯技术得到纯化的鼠李糖脂的单糖脂和二糖脂,最后采用高效液相色谱-质谱联用法进行成分鉴定。结果显示这两种鼠李糖脂均含有3种主要成分,其中单糖脂的主要成分为RhaC10C10、RhaC10C12-H2、RhaC10C12,二糖脂的主要成分为Rha2C10C10、Rha2C10C12-H2、Rha2C10C12。该研究结果表明,铜绿假单胞菌CCTCC AB93066是一种良好的鼠李糖脂产生菌;酸沉降-柱色谱技术可以用于鼠李糖脂的深度提纯,且有较好的效果;而高效液相色谱-质谱联用技术对鼠李糖脂成分鉴定具有灵敏度高和准确性好等优点,是一种较为可靠的检测方法。

English

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  • 收稿日期:  2013-10-29
  • 网络出版日期:  2013-12-20
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