Citation: ZHU Feng, RUAN Liping, MA Yongjian, JI Wenliang, LIU Hualiang. Simultaneous determination of 20 illegally added anti-diabetic chemical components in hypoglycemic and weight-reducing health foods by ultra-high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(1): 13-20. doi: 10.3724/SP.J.1123.2013.08035 shu

Simultaneous determination of 20 illegally added anti-diabetic chemical components in hypoglycemic and weight-reducing health foods by ultra-high performance liquid chromatography-tandem mass spectrometry

1.
Jiangsu Provincial Center for Disease Prevention and Control, Nanjing 210009, China

Corresponding author: LIU Hualiang,
Received Date: 21 August 2013
Available Online: 23 September 2013
Fund Project: 江苏科技厅科技支撑计划项目(BE2010745) (BE2010745)江苏省“十二五”科教兴卫工程项目(ZX201109). (ZX201109)

A rapid method for the simultaneous screening and detection of 20 illegally added anti-diabetic chemical components in hypoglycemic and weight-reducing health foods was developed by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). After extracted by methanol, the sample was separated on a Poroshell 120 EC C18 column (100 mm×2.1 mm, 2.7 μm) with the gradient elution of 5 mmol/L ammonium acetate and acetonitrile as mobile phases. The electrospray ionization (ESI) source in positive or negative ion mode was used for multiple reaction monitoring (MRM) mode. The 20 illegally added chemical components showed good linear relationships with the correlation coefficients more than 0.99. The recoveries were in the range of 75.9%-114.0%, and the relative standard deviations (RSDs) were all not more than 11.3%. The limits of detection (LODs) were all in the range of 0.3-1.5 μg/L. This method is rapid, simple, sensitive, accurate and of good specificity for cracking down illegally added anti-diabetic chemical components.
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沈阳化工大学材料科学与工程学院沈阳 110142