Citation: BIAN Kui, LIN Tao, LIU Min, YANG Jianwen, WANG Zongnan, HE Limin. Matrix effects in analysis of three β-agonist residues in pig edible tissues using gas chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, ;2014, 32(2): 162-168. doi: 10.3724/SP.J.1123.2013.08031 shu

Matrix effects in analysis of three β-agonist residues in pig edible tissues using gas chromatography-mass spectrometry

BIAN Kui ¹ ,
LIN Tao ² ,
LIU Min ¹ ,
YANG Jianwen ¹ ,
WANG Zongnan ¹ ,
HE Limin ^1,,

1.
1. Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, South China Agriculture University, Guangzhou 510642, China;

Corresponding author: HE Limin,
Received Date: 19 August 2013
Available Online: 24 September 2013
Fund Project: 国家自然科学基金项目(31072166) (31072166)东莞市高等院校科研机构科技项目(2011108102007). (2011108102007)

A gas chromatography-mass spectrometry (GC-MS) method was established for the determination of the residues of three β-agonists (clenbuterol, salbutamol and ractopamine) in pig edible tissues. The matrix effects (MEs) in the analysis of the three compounds with the developed method were determined. The influences of matrix state and its weight on MEs were evaluated statistically. The analytes in pig liver and muscle and their corresponding freeze-dried powders were derivatized with N,O-bis(trimethylsilyl) trifluoroacetamide. Then the derivatives were determined in selected ion monitoring mode and the intensities of MEs of the three β-agonists were obtained. Significant matrix enhancement was observed for the three analytes, and especially, the ME of ractopamine was more than 1000%. The results of analysis of variance (ANOVA) demonstrated that MEs were significantly different for the three analytes in two matrices among different matrix weights (P < 0.05), and MEs of the three analytes increased from 1 g to 5 g with the increase of matrix weight. MEs for the three analytes were not significantly different between fresh pig tissues and its freeze-dried powder matrices (P > 0.05), indicating that the freeze-dried powder matrices might be used to conveniently prepare the matrix-matched calibration solution, which could efficiently compensate the MEs of the β-agonists in GC-MS analysis.
- gas chromatography-mass spectrometry (GC-MS),
- β-agonists,
- pig tissues,
- matrix effect,
- analysis of variance (ANOVA)
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