Citation: ZHANG Yong, XUE Kunpeng, ZHOU An, YU Huimin, LI Yin, YAO Lixin, ZHAO Yuexing, XIE Xiaomei. Determination of triterpenoic acid isomers in Eriobotryae folium using high performance liquid chromatography on C₃₀ column[J]. Chinese Journal of Chromatography, ;2014, 32(1): 47-51. doi: 10.3724/SP.J.1123.2013.08019 shu

Determination of triterpenoic acid isomers in Eriobotryae folium using high performance liquid chromatography on C₃₀ column

ZHANG Yong ^1,, ,
XUE Kunpeng ² ,
ZHOU An ¹ ,
YU Huimin ¹ ,
LI Yin ² ,
YAO Lixin ² ,
ZHAO Yuexing ² ,
XIE Xiaomei ¹

1.
1. School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230031, China;

Corresponding author: ZHANG Yong,
Received Date: 13 August 2013
Available Online: 10 September 2013
Fund Project: 安徽省教育厅自然科学基金项目(KJ2013A172) (KJ2013A172)安徽中医学院自然科学基金项目(2012zr023). (2012zr023)

A rapid and easy assay method for four triterpenoic acid isomers in Eriobotryae folium was established. The sample was extracted with methanol, then separated on a C₃₀ column (250 mm×4.6 mm, 5 μm) at 20 ℃ using acetonitrile-water (95:5, v/v) as the mobile phase at a flow rate of 1.0 mL/min. The target compounds were simultaneously detected at 210 nm, and the injection volume was 10 μ L. The validation was carried out and the resolution (R≥2.2), the precision (RSD≤1.1%), the linearity (r≥0.9992), the repeatability (RSD≤4.4%), and the recovery (range from 95.4% to 101.7%, RSD≤4.8%) were acceptable. The method is reliable for the quantification of the four triterpenoic acid isomers by HPLC on C₃₀ column.
- high performance liquid chromatography (HPLC),
- C₃₀ column,
- maslinic acid,
- corosolic acid,
- oleanolic acid,
- ursolic acid,
- isomers,
- Eriobotryae folium
1. [1]
  [1] Guo Y, Wu S J, Piao H S. Lishizhen Medicine and Materia Medica Research (郭宇, 吴松吉, 朴惠善. 时珍国医国药), 2006, 17(6): 928
2. [2]
  [2] Zhang Y, Wang J Q. Food Science (张玉, 王建清. 食品科学), 2005, 26(9): 602
3. [3]
  [3] Ge J F, Li J, Yao H W, et al. Acta Universitatis Medicinalis Anhui (葛金芳, 李俊, 姚宏伟, 等. 安徽医科大学学报), 2007, 42(2): 174
4. [4]
  [4] Li F, Wang H, Xue Y K, et al. Pharmaceutical Biotechnology (李锋, 王航, 薛原楷, 等. 药物生物技术), 2011, 18(4): 328
5. [5]
  [5] Li X G. China Modern Medicine (黎雪桂. 中国当代医药), 2012, 19(11): 17
6. [6]
  [6] Chen L S, Xin Y, Xu S W, et al. Chinese Traditional and Herbal Drugs (陈龙胜, 忻旸, 许舒雯, 等. 中草药), 2008, 39(9): 1411
7. [7]
  [7] Qi Y F, Li K. Chinese Traditional and Herbal Drugs (戚雁飞, 李鲲. 中草药), 2011, 42(6): 1217
8. [8]
  [8] Zhang S L, Xie X M, Cheng J J, et al. Chinese Pharmaceutical Journal (张圣龙, 谢晓梅, 程菁菁, 等. 中国药学杂志), 2013, 48(6): 419
9. [9]
  [9] Pharmacopoeia Commission of People's Republic of China. Pharmacopoeia of People's Republic of China, Part 1. Beijing: Chinese Medical Science and Technology Press (国家药典委员会. 中华人民共和国药典, 一部. 北京: 中国医药科技出版社), 2010: 190
10. [10]
  [10] Lü H, Xi C P, Chen J, et al. China Journal of Chinese Materia (吕寒, 习超鹏, 陈剑, 等. 中国中药杂志), 2009, 34(18): 2353
11. [11]
  [11] Hu C P, Chen L S, Xin Y. Chinese Journal of Chromatography (胡长平, 陈龙胜, 忻旸. 色谱), 2006, 24(5): 492
12. [12]
  [12] Cai X P, Li S L, Hua J L, et al. Chinese Journal of Experimental Traditional Medicine Formulae (蔡雪萍, 李松林, 华俊磊, 等. 中国实验方剂学杂志), 2013, 19(1): 86
13. [13]
  [13] Lü H, Xi C P, Chen J, et al. Chinese Traditional Patent (吕寒, 习超鹏, 陈剑, 等. 中成药), 2009, 31(1): 89
14. [14]
  [14] Zha X Z, Xie X M, Lü M H, et al. Chinese Journal of Experimental Traditional Medical Formulae (査孝柱, 谢晓梅, 吕美红, 等. 中国实验方剂学杂志), 2010, 16(18): 60
15. [15]
  [15] Wang H H, Wang Z Z, Guo W B. Ind Crop Prod, 2008, 28(3): 328
16. [16]
  [16] Willy H, Ruud H S, Peter R K. J Chromatogr A, 1995, 697(1/2): 165
17. [17]
  [17] Purcaro G, Moret S, Bucar-Miklavcic M C. J Sep Sci, 2012, 35(8): 922
18. [18]
  [18] Lesellier E. J Chromatogr A, 2011, 1218(2): 251
19. [19]
  [19] Hui B D, Li J, Sun N N, et al. Chinese Journal of Food Hygiene (惠伯棣, 李京, 孙拿拿, 等. 中国食品卫生杂志), 2006, 18(4): 289
20. [20]
  [20] Pei L P, Hui B D, Liu R, et al. Food Science (裴凌鹏, 惠伯棣, 刘蕊, 等. 食品科学), 2006, 27(10): 323
21. [21]
  [21] Peng J, Xiang W Z, Tang Q M, et al. Science in China Series C: Life Sciences, 2008, 51(12): 1108
22. [22]
  [22] Chen L S, Lü Y, Xu S W, et al. Lishizhen Medicine and Materia Medica Research (陈龙胜, 吕杨, 许舒雯, 等. 时珍国医国药), 2008, 19(12): 2909
23. [23]
  [23] Liu B, Wu H Z. Hubei Journal of Traditional Chinese Medicine (刘博, 吴和珍. 湖北中医杂志), 2010, 32(12): 75
1. [1]
  Tong Wang , Liangyu Hu , Shiqi Chen , Xinqiang Fu , Rui Wang , Kun Li , Shuangyan Huan . Determination of Benzenediol Isomers in Cosmetics Using High-Performance Liquid Chromatography Empowered by “Mathematical Separation”. University Chemistry, 2026, 41(1): 9-19. doi: 10.12461/PKU.DXHX202503128
2. [2]
  Qi Liang , Xinxin Wang , Xinmiao Zhao , Mohan Yu , Yue Sun . 电子圆二色谱结合量子化学计算的氨基酸手性解析——介绍一个计算化学综合实验. University Chemistry, 2026, 41(5): 380-390. doi: 10.12461/PKU.DXHX202511096
3. [3]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
4. [4]
  Lingyu Chang , Yanfang Lang , Yuyan Zhu , Jie Wang , Ying Guo , Die Wang , Peng Ding , Yueming Zhou , Zhixiang Gong , Shujuan Liu . Machine Learning-Optimized Microcolumn Ion Exchange Chromatography for Trace Arsenic Determination. University Chemistry, 2026, 41(1): 76-84. doi: 10.12461/PKU.DXHX202506023
5. [5]
  Ping Song , Nan Zhang , Jie Wang , Rui Yan , Zhiqiang Wang , Yingxue Jin . Experimental Teaching Design on Synthesis and Antitumor Activity Study of Cu-Pyropheophorbide-a Methyl Ester. University Chemistry, 2024, 39(6): 278-286. doi: 10.3866/PKU.DXHX202310087
6. [6]
  Ran Yu , Chen Hu , Ruili Guo , Ruonan Liu , Lixing Xia , Cenyu Yang , Jianglan Shui . Catalytic Effect of H₃PW₁₂O₄₀ on Hydrogen Storage of MgH₂. Acta Physico-Chimica Sinica, 2025, 41(1): 100001-0. doi: 10.3866/PKU.WHXB202308032
7. [7]
  Mengyang LI , Hao XU , Zhonghao NIU , Chunhua GONG , Weihui ZHONG , Jingli XIE . Highly effective catalytic synthesis of β-amino alcohols by using viologen-polyoxometalate hybrid materials. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1294-1300. doi: 10.11862/CJIC.20250080
8. [8]
  Jianquan Liu , Xiangshan Wang . Teaching Design and Practice of Naming Rules for Circular Isomer Configuration under the Guidance of Information Literacy. University Chemistry, 2025, 40(7): 352-358. doi: 10.12461/PKU.DXHX202409082
9. [9]
  Qingying Gao , Tao Luo , Jianyuan Su , Chaofan Yu , Jiazhu Li , Bingfei Yan , Wenzuo Li , Zhen Zhang , Yi Liu . Refinement and Expansion of the Classic Cinnamic Acid Synthesis Experiment. University Chemistry, 2024, 39(5): 243-250. doi: 10.3866/PKU.DXHX202311074
10. [10]
  Lanjun Cheng , Xinyuan Wang , Jie An , Xiang Wu , Chengfeng Zhu , Yanming Fu , Yougui Li . Improvement of the Resolution Experiment of Racemic Tartaric Acid. University Chemistry, 2025, 40(7): 277-285. doi: 10.12461/PKU.DXHX202408010
11. [11]
  Zhi Zheng , Feiyang Liu , Junlong Zhao . D-Amino Acids and Mirror-Image Proteins. University Chemistry, 2026, 41(2): 353-359. doi: 10.12461/PKU.DXHX202505017
12. [12]
  Yuan Zheng , Quan Lan , Zhenggen Zha , Lingling Li , Jun Jiang , Pingping Zhu . Teaching Reform of Organic Synthesis Experiments by Introducing Reverse Thinking and Design Concepts: Taking the Synthesis of Cinnamic Acid Based on Retrosynthetic Analysis as an Example. University Chemistry, 2024, 39(6): 207-213. doi: 10.3866/PKU.DXHX202310065
13. [13]
  Conghao Shi , Ranran Wang , Juli Jiang , Leyong Wang . The Illustration on Stereoisomers of Macrocycles Containing Multiple Chiral Centers via Tröger Base-based Macrocycles. University Chemistry, 2024, 39(7): 394-397. doi: 10.3866/PKU.DXHX202311034
14. [14]
  Xiaolei Jiang , Fangdong Hu . Exploring the Mirror World in Organic Chemistry: the Teaching Design of “Enantiomers” from the Perspective of Curriculum and Ideological Education. University Chemistry, 2024, 39(10): 174-181. doi: 10.3866/PKU.DXHX202402052
15. [15]
  Renqing Lü , Shutao Wang , Fang Wang , Guoping Shen . Computational Chemistry Aided Organic Chemistry Teaching: A Case of Comparison of Basicity and Stability of Diazine Isomers. University Chemistry, 2025, 40(3): 76-82. doi: 10.12461/PKU.DXHX202404119
16. [16]
  Haiyuan Wang , Xiaoning Jin , Yajing Sun , Zhen Zhang , Wentao Zhao , Yi Li . Practical Exploration of High-Performance Liquid Chromatography Experiment Teaching Reform Empowered by Artificial Intelligence. University Chemistry, 2026, 41(4): 45-51. doi: 10.12461/PKU.DXHX202505076
17. [17]
  Peng XU , Shasha WANG , Nannan CHEN , Ao WANG , Dongmei YU . Preparation of three-layer magnetic composite Fe₃O₄@polyacrylic acid@ZiF-8 for efficient removal of malachite green in water. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 544-554. doi: 10.11862/CJIC.20230239
18. [18]
  Meijin Li , Xirong Fu , Xue Zheng , Yuhan Liu , Bao Li . The Marvel of NAD⁺: Nicotinamide Adenine Dinucleotide. University Chemistry, 2024, 39(9): 35-39. doi: 10.12461/PKU.DXHX202401027
19. [19]
  Keying Qu , Jie Li , Ziqiu Lai , Kai Chen . Unveiling the Mystery of Chirality from Tartaric Acid. University Chemistry, 2024, 39(9): 369-378. doi: 10.12461/PKU.DXHX202310091
20. [20]
  Jianqiang Zheng , Yongbin Huang , Wencan Ming , Yingju Liu . Intelligent Reaction Optimization: Synthesis of Acetylsalicylic Acid Driven by Deep Learning and Optimization Algorithms. University Chemistry, 2025, 40(9): 87-98. doi: 10.12461/PKU.DXHX202411062

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(695)
HTML views(65)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Determination of triterpenoic acid isomers in Eriobotryae folium using high performance liquid chromatography on C30 column

Metrics

通讯作者: 陈斌, bchen63@163.com

Determination of triterpenoic acid isomers in Eriobotryae folium using high performance liquid chromatography on C₃₀ column