Citation: ZHOU Xianjing, SHI Yanping. Determination of amino acids in honey by capillary electrophoresis with indirect ultraviolet detection[J]. Chinese Journal of Chromatography, ;2013, 31(7): 661-666. doi: 10.3724/SP.J.1123.2013.05027 shu

Determination of amino acids in honey by capillary electrophoresis with indirect ultraviolet detection

ZHOU Xianjing ^1,2 ,
SHI Yanping ^1,,

1.
1. Key Laboratory of Chemistry of Northwestern Plant Resources, CAS/Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China;

Corresponding author: SHI Yanping,
Received Date: 16 May 2013

Fund Project: 国家自然科学基金项目(21075127). (21075127)

A method of capillary electrophoresis with indirect ultraviolet (UV) detection was developed for the separation and determination of nine amino acids such as lysine, tryptophan, glutamic acid, etc. The effects of sodium dihydrogen phosphates concentration, pH of buffer and sample injection type and time on the reproducibility and efficiency were investigated. The optimum injection time was 5 s at 5 kPa. The optimum electrophoretic conditions were as follow: 10 mmol/L sodium dihydrogen phosphates (pH 10.2) containing 0.5 mmol/L cetrimonium bromide, 20 mmol/L nicotinic acid and 10%(v/v) methanol as running buffer, applied voltage of-15 kV, detection wavelength of 220 nm. The base line separation of the nine amino acids was achieved successfully within 11 min. The lowest detection limit was 0.3 mg/L. All of the nine analytes showed good linearities within 1.0-1000 mg/L. The relative standard deviations of migration time and peak area were 0.64%-5.83%. The recoveries of the eight amino acids spiked in a real sample were between 60.00% and 118.37%. The method was applied in the determination of the amino acids in honey samples from different nectar plants and origins. Prolin, serine and aspartic acid were found in five honey samples, and tryptophan was only found in a litchi honey sample. This method can provide good reference to the evaluation of the quality and nectar origin of honey.
- capillary electrophoresis (CE),
- indirect ultraviolet detection,
- amino acids,
- honey
1. [1]
  [1] Blasa M, Candiracci M, Accorsi A, et al. Food Chem, 2007, 104: 1635
2. [2]
  [2] Lachman J, Orsák M, Hejtmánková A, et al. LWT-Food Sci Technol, 2010, 43: 52
3. [3]
  [3] Boorn K L, Khor Y Y, Sweetman E, et al. J Applied Micro, 2010, 108: 1534
4. [4]
  [4] Dong R, Zheng Y N. Food Science (董蕊, 郑毅男. 食品科学), 2011, 32(21): 66http://mall.cnki.net/magazine/Article/SPKX201121014.htm
5. [5]
  [5] Cotte J F, Casabianca H, Giroud B, et al. Anal Bioanal Chem, 2004, 378(5): 1342
6. [6]
  [6] Hamide Z, Senyuva H Z, Gilbert J, et al. J Agric Food Chem, 2009, 57(9): 3911
7. [7]
  [7] Katarzyna J, Magda A, Piotr N. Pol J Food Nutr Sci, 2012, 62(2): 85
8. [8]
  [8] Sun Y C, Xu X Z, Xu Y L, et al. Chinese Journal of Chromatography (孙言春, 许宪祝, 徐衍岭, 等. 色谱), 2013, 31(3): 275
9. [9]
  [9] Feng L, Yan A P, Wan Y Q, et al. Chinese Journal of Chromatography (冯蕾, 鄢爱平, 万益群, 等. 色谱), 2010, 28(4): 408
10. [10]
  [10] Tang X X, Gu Y, Wang C, et al. Chinese Journal of Chromatography (汤新星, 顾源, 王畅, 等. 色谱), 2012, 30(7): 696
11. [11]
  [11] Wang Y H, Shen K Y, Li P, et al. Chinese Journal of Chromatography (王玉红, 申克宇, 李鹏, 等. 色谱), 2011, 29(9): 908
12. [12]
  [12] Pereira V, Pontes M, Camara J S, et al. J Chromatogr A, 2008, 1189(1/2): 435
13. [13]
  [13] Huang Y T, Chen P C, Chen R L C, et al. Food Chem, 2010, 118(3): 876
14. [14]
  [14] Hu J H, Qiu L Y, Wang C R, et al. Food Science and Technology (胡建鸿, 邱利焱, 王成润, 等. 食品科技), 2008(10): 211http://www.cnki.com.cn/Journal/B-B6-SSPJ-2008-10.htm
15. [15]
  [15] Rebane R, Herodes K. J Chromatogr A, 2012, 1245(6): 134
16. [16]
  [16] Huang H, Gong X H, Liu H H, et al. Food Science (黄会, 宫向红, 刘慧慧, 等. 食品科学), 2012, 33(8): 207http://mall.cnki.net/magazine/magadetail/SPKX201208.htm
17. [17]
  [17] Fu C G, Yang D Z, Wang L X. Food Science (傅崇岗, 杨冬芝, 王立新. 食品科学), 2005, 26(12): 196http://www.cnki.com.cn/Journal/B-B6-SPKX-2005-12.htm
18. [18]
  [18] Fu G N, He Y Z, Wang X K, et al. Chinese Journal of Chromatography (付国妮, 何友昭, 王晓葵, 等. 色谱), 2007, 25(2): 193
19. [19]
  [19] Chen B, Li X G, He P, et al. Chinese Journal of Chromatography (陈冰, 李小戈, 何萍, 等. 色谱), 2004, 22(1): 74
20. [20]
  [20] Yan H S, Cheng X H, He B L. Chemical Journal of Chinese Universities (阎虎生, 程晓辉, 何炳林. 高等学校化学学报), 1992, 13(2): 270http://www.cjcu.jlu.edu.cn/CN/abstract/abstract21109.shtml
21. [21]
  [21] Liu J X, Liu G D, Yan H S, et al. Chemical Journal of Chinese Universities (刘菊湘, 刘国栋, 阎虎生, 等. 高等学校化学学报), 2001, 22(12): 2100http://wuxizazhi.cnki.net/Article/GDXH200112037.html
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