Citation: GUO Chengye, WANG Houyu, ZHANG Lei, FAN Liuyin, CAO Chengxi. Determination of total protein content in soya-bean milk via visual moving reaction boundary titration[J]. Chinese Journal of Chromatography, ;2013, 31(11): 1064-1070. doi: 10.3724/SP.J.1123.2013.04040 shu

Determination of total protein content in soya-bean milk via visual moving reaction boundary titration

GUO Chengye ^1,2 ,
WANG Houyu ² ,
ZHANG Lei ^1,, ,
FAN Liuyin ² ,
CAO Chengxi ^2,,

1.
1. College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China;

Corresponding author: ZHANG Lei, CAO Chengxi,
Received Date: 23 April 2013
Available Online: 26 May 2013
Fund Project: 科技部重大仪器专项基金项目(2011YQ030139) (2011YQ030139)国家自然科学基金项目(21275099,21035004). (21275099,21035004)

A visual, rapid and accurate moving reaction boundary titration (MRBT) method was used for the determination of the total protein in soya-bean milk. During the process, moving reaction boundary (MRB) was formed by hydroxyl ions in the catholyte and soya-bean milk proteins immobilized in polyacrylamide gel (PAG), and an acid-base indicator was used to denote the boundary motion. The velocity of MRB has a relationship with protein concentration, which was used to obtain a standard curve. By paired t-test, there was no significant difference of the protein content between MRBT and Kjeldahl method at 95% confidence interval. The procedure of MRBT method required about 10 min, and it had linearity in the range of 2.0-14.0 g/L, low limit of detection (0.05 g/L), good precision (RSD of intra-day<1.90% and inter-day<4.39%), and high recoveries (97.41%-99.91%). In addition, non-protein nitrogen (NPN) such as melamine added into the soya-bean milk had weak influence on MRBT results.
- moving reaction boundary,
- electrophoresis,
- titration,
- total protein content,
- soya-bean milk
1. [1]
  [1] Thompson M, Owen L, Wilkinson K, et al. Analyst, 2002, 127(12): 1666
2. [2]
  [2] Moore J C, DeVries J W, Lipp M, et al. Comper Rev Food Sci Food Saf, 2010, 9(4): 330
3. [3]
  [3] Lam C W, Lan L, Che X, et al. Clin Chim Acta, 2009, 402(1): 150
4. [4]
  [4] Trivedi U, Lakshminarayana D, Kothari I, et al. Sensor Actuator B: Chem, 2009, 140(1): 260
5. [5]
  [5] GB/T 22106-2008
6. [6]
  [6] Deman J. Anal Chem, 1970, 42(3): 321
7. [7]
  [7] Deman J. Anal Chem, 1970, 42(14): 1699
8. [8]
  [8] Cao C X. J Chromatogr A, 1998, 813(1): 153
9. [9]
  [9] Cao C X, Fan L Y, Zhang W. Analyst, 2008, 133(9): 1139
10. [10]
  [10] Yang Q, Fan L Y, Huang S S, et al. Electrophoresis, 2011, 32(9): 1015
11. [11]
  [11] Wang H Y, Yang Q, Dong J Y, et al. Chinese Journal of Analytical Chemistry (王后禹, 杨清, 董靖宇, 等. 分析化学), 2012, 40(6): 968
12. [12]
  [12] Guo C Y, Wang H Y, Liu X P, et al. Electrophoresis, DOI: 10.1002/elps.201300007
13. [13]
  [13] McGoverin C M, Clark A S S, Holroyd S E, et al. Anal Chim Acta, 2010, 673(1): 26
14. [14]
  [14] Pace C N, Grimsley G R, Scholtz J M. J Biol Chem, 2009, 284(20): 13285
15. [15]
  [15] Duso A B, Chen D D Y. Anal Chem, 2002, 74(13): 2938
16. [16]
  [16] Huang T, Wu X Z, Pawliszyn J. Anal Chem, 2000, 72(19): 4758
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