Citation: ZHU Weixia, YANG Jizhou, YUAN Ping, SUN Zhuanlian, WANG Caijuan, SUN Wuyong, ZHANG Shusheng. Rapid determination of total residues of ribavirin and its metabolites in chicken and chicken products by ultra-performance hydrophilic interaction chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2013, 31(10): 934-938. doi: 10.3724/SP.J.1123.2013.01048 shu

Rapid determination of total residues of ribavirin and its metabolites in chicken and chicken products by ultra-performance hydrophilic interaction chromatography-tandem mass spectrometry

1.
1. Henan Entry-Exit Inspection and Quarantine Bureau, Zhengzhou 450003, China;

Corresponding author: YANG Jizhou,
Received Date: 30 March 2013
Available Online: 17 May 2013
Fund Project: 河南省科技攻关项目(122102110167) (122102110167)质检总局科研项目(2007IK162). (2007IK162)

An analytical method was developed for the determination of total residues of ribavirin and its phosphorylated metabolites in chicken and its products by hydrophilic interaction chromatography-tandem mass spectrometry. The samples were extracted with acetonitrile containing 1% (v/v) acetic acid under ultrasonic condition and then enzymatically hydrolysed with acid phosphatase at 37 ℃. After liposoluble substances being removed with hexane, C₁₈ and PSA dispersion solid phase extraction (DSPE) was introduced to cleanup procedures. The separation was performed on an ultra-performance hydrophilic interaction chromatographic (HILIC) amide column under a gradient elution using acetonitrile and 0.1% formic acid. The analytes were detected in the positive electrospray ionization and multi-reaction monitoring (MRM) mode. In the range of 1-200 μg/kg, the correlation coefficient was greater than 0.999. The limit of detection (LOD, S/N≥3) was 1 μg/kg and the limit of quantification (LOQ, S/N≥10) was 5 μg/kg. The recoveries of ribavirin spiked at three levels ranged from 67.8% to 112.7% with the relative standard deviations (RSDs) of 6.1%-13.6%. The results of the determination of ribavirin in various real samples showed that the method is simple, rapid, accurate and suitable for the determination of total residues of ribavirin and its metabolites in chicken and its products.
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