Citation: YUN Huan, CUI Fengyun, YAN Hua, LIU Xin, HE Yue, ZHANG Zhaohui. Determination of ribavirin and amantadine in chicken by ultra performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2013, 31(8): 724-728. doi: 10.3724/SP.J.1123.2013.01023 shu

Determination of ribavirin and amantadine in chicken by ultra performance liquid chromatography-tandem mass spectrometry

1.
Technology Center of Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, China

Corresponding author: YUN Huan,
Received Date: 10 January 2013
Available Online: 11 March 2013
Fund Project: 国家质检总局科研项目(2013IK146). (2013IK146)

A method for the determination of ribavirin and amantadine in chicken has been developed by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). After extracted by 1% (volume percentage) trichloroacetic acid solution-acetonitrile (1:1, v/v) and purified by a Supelco LC-SCX cartridge, the samples were loaded onto an Acquity UPLC BEH Hillic column (150 mm×2.1 mm, 1.7 μm)and separated with gradient elution. The electrospray was operated in the positive mode and the samples were monitored by the multiple reaction monitoring (MRM) mode. The limits of quantification (LOQs, S/N=10) of ribavirin and amantadine were 4.0 μg/kg. The calibration curves showed good linearity in the range of 10.0-100.0 μg/L, and the correlation coefficients (r²) were not lower than 0.99. When the spiked levels were 4.0, 8.0 and 20.0 μg/kg, the recoveries of ribavirin and amantadine in chicken ranged from 78% to 102.5%, with the relative standard deviations (RSDs) of 2.2%-7.6%. The results indicate that the method is simple, rapid, sensitive and suitable for the qualitative and quantitative analyses of ribavirin and amantadine in chicken samples.
- ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS),
- ribavirin,
- amantadine,
- chicken
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沈阳化工大学材料科学与工程学院沈阳 110142