Citation: ZHOU Hongbin, XIONG Zhiyu, YU Yang, WAN Rong, LI Ping, SHEN Bo. Determination of alditols in wine by gas chromatography-mass spectrometry after acetate derivatization[J]. Chinese Journal of Chromatography, ;2013, 31(8): 786-790. doi: 10.3724/SP.J.1123.2013.01005 shu

Determination of alditols in wine by gas chromatography-mass spectrometry after acetate derivatization

ZHOU Hongbin ^1,, ,
XIONG Zhiyu ² ,
YU Yang ¹ ,
WAN Rong ¹ ,
LI Ping ¹ ,
SHEN Bo ¹

1.
1. State Key Laboratory of Food Additive and Condiment Testing, Zhenjiang 212008, China;

Corresponding author: ZHOU Hongbin,
Received Date: 5 January 2013
Available Online: 26 January 2013
Fund Project: 国家质量监督检验检疫总局公益性科研项目(2012424012-3). (2012424012-3)

The acetate derivatization of alditols for determining alditol level in wine by gas chromatography (GC)-mass spectrometry (MS) has been developed. The wine sample was mixed with pyridine and centrifuged at 5000 r/min at the temperature of 4℃ for 10 min. After filtration with organic phase membrane, the supernatant was derivatized with acetic anhydride, and then dehydrated with anhydrous sodium sulfate. The GC separation was performed on a DB-5MS capillary column. The alditols were determined by MS in selected ion monitoring (SIM) mode and quantified by external standard method. The calibration curves showed good linearities in the range of 0.019-1.25 mg/L except for lactitol (0.039-2.50 mg/L) with the correlation coefficients greater than 0.99. The limits of quantification (S/N=10) of erythritol, xylitol, D-mannitol, sorbitol, galactitol and lactitol were 0.17, 0.29, 0.43, 0.46, 0.47 and 2.88 mg/L respectively. The limits of detection (S/N=3) were 0.05, 0.08, 0.13, 0.14, 0.14 and 1.38 mg/L respectively. The recoveries of alditols spiked in the wine at two levels of 40 mg/L and 80 mg/L were ranged from 80.15% to 108.75% with the relative standard deviations (RSDs) of 2.16%-6.97%. The sensitivity, accuracy and precision of the method can meet the technical standard. The method can be applied to the rapid determination of alditols in wine.
- derivatization,
- gas chromatography-mass spectrometry (GC-MS),
- alditol,
- wine
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