Citation: XIE Honglei, LI Chun, LIU Ning. Analysis of sialic acid in infant formulas using ultra performance liquid chromatography-triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, ;2013, 31(8): 781-785. doi: 10.3724/SP.J.1123.2012.12051 shu

Analysis of sialic acid in infant formulas using ultra performance liquid chromatography-triple quadrupole mass spectrometry

XIE Honglei ^1,2 ,
LI Chun ^1,, ,
LIU Ning ^1,2,,

1.
1. College of Food Sciences, Northeast Agricultural University, Key Laboratory of Dairy Science, Ministry of Education, Harbin 150030, China;

Corresponding author: LI Chun, LIU Ning,
Received Date: 28 December 2012
Available Online: 1 February 2013
Fund Project: "十二五"国家科技支撑课题(2011BAD09B0304). (2011BAD09B0304)

The method for analysing sialic acid in infant formulas by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) has been established. Sialic acid in milk was released via acid hydrolysis, and purified by an HLB solid phase extraction cartridge. The UPLC separation was performed on an ACQUITY UPLC^TM BEH HILIC column (50 mm×2.1 mm, 1.7 μm) utilizing a gradient elution program of acetonitrile and water (containing 0.1% formic acid) as the mobile phases at a flow rate of 0.25 mL/min. Injection volume and column temperature were set at 5 μL and 30℃, respectively. The identification and quantification were achieved by using electrospray ionisation (ESI)-MS/MS in positive ion mode and multiple reaction monitoring (MRM) mode. The linear range was from 0.05 to 5.0 mg/L for sialic acid and the correlation coefficient (R²) was greater than 0.99. The average recoveries spiked at the four concentration levels of 0.1, 0.5, 2.5 and 5.0 mg/L ranged between 84.3% and 98.9% with the relative standard deviations from 4.9% to 8.2%. The limit of detection was 0.01 mg/L. Therefore, this method has the characteristics of simple operation, high reproducibility and sensitivity. It can be widely applied to determine the total contents of sialic acid in infant formula, cow milk and human milk.
- ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS),
- sialic acid,
- infant formula
1. [1]
  [1] Wang B, Brand-Milleret J. Eur J Clin Nutr, 2003, 57(11): 1351
2. [2]
  [2] Wang B, McVeagh P, Petocz P, et al. Am J Clin Nutr, 2003, 78(5): 1024
3. [3]
  [3] Carlson S E. Am J Clin Nutr, 1985, 41: 720
4. [4]
  [4] Kikuchi H, Kikuchi H, Tsuiki S. Biochim Biophys Acta, 1971, 252(2): 357
5. [5]
  [5] Wang B, Brand-Milleret J, McVeagh P, et al. Am J Clin Nutr, 2001, 74(4): 510
6. [6]
  [6] Martin-Sosa S, Martin M J, Garcia-Pardo L A, et al. J Dairy Sci, 2003, 86(1): 52
7. [7]
  [7] Zhang X M. Foreign Medical Sciences: Section of Clinical Biochemistry and Laboratory Medicine (张秀明. 国外医学: 临床生物化学与检验学分册), 1995, 16(2): 75
8. [8]
  [8] Huang H J, Xi X L, Wu H Z, et al. Journal of Instrumental Analysis (黄华军, 奚星林, 吴宏中, 等. 分析测试学报), 2006, 25(4): 129
9. [9]
  [9] Chen H J, Wang P, Chen Y, et al. Food Science (陈海娇, 王萍, 陈跃, 等. 食品科学), 2011, 32(16): 308
10. [10]
  [10] Salcedo J, Lacomba R, Alegría A, et al. Food Chem, 2011, 127(4): 1905
11. [11]
  [11] Spichtig V, Michaud J, Austin S. Anal Biochem, 2010, 405(1): 28
12. [12]
  [12] Li H, Jin Y B, Liu H X, et al. Journal of Instrumental Analysis (栗晖, 金一宝, 刘红霞, 等. 分析测试学报), 2008, 27(Suppl): 193
13. [13]
  [13] Tebani A, Schlemmer D, Imbard A, et al. J Chromatogr B, 2011, 879(31): 3694
14. [14]
  [14] van der Ham M, de Koning T J, Lefeber D, et al. J Chromatogr B, 2010, 878(15/16): 1098
15. [15]
  [15] van der Hama V, Prinsen B H C M T, Huijmans J G M, et al. J Chromatogr B, 2007, 848(2): 251
16. [16]
  [16] Food and Drug Administration. Guidance for Industry, Bioanalytical Method Validation. (2001-03). http://www.fda.gov/cder/guidance/index.htm
17. [17]
  [17] Sinha D, Chatterjee M, Mandal C. Trends in Glycoscience and Glycotechnology, 2000, 12(63): 17
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