Citation: ZHU Lin, RUAN Liping, LIU Hualiang, JI Wenliang, MA Yongjian. Simultaneous determination of 23 sedative drugs in health foods by high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, ;2013, 31(7): 709-713. doi: 10.3724/SP.J.1123.2012.12025 shu

Simultaneous determination of 23 sedative drugs in health foods by high performance liquid chromatography-tandem mass spectrometry

ZHU Lin ¹ ,
RUAN Liping ² ,
LIU Hualiang ² ,
JI Wenliang ² ,
MA Yongjian ^1,2,,

1.
1. School of Public Health, Southeast University, Nanjing 210009, China;

Corresponding author: MA Yongjian,
Received Date: 19 December 2012

Fund Project: 江苏省科技厅科技支撑计划项目(BE2010745). (BE2010745)

An analytical method using HPLC-MS/MS was developed for qualitative and quantitative analysis of 23 sedative drugs in health foods. The method was based on the sonication-assisted extraction of the health food samples using 10 mL methanol. The extract was then isolated by centrifugation and the supernatant was separated on an Agilent Eclipse Plus C₁₈ column with gradient elution at a flow rate of 300 μ L/min. A binary mobile phase was 10 mmol/L ammonium formate (solvent A) and acetonitrile/methanol (1:1, v/v; solvent B). The electrospray ionization (ESI) source in positive ion mode or negative ion mode was used for multiple reaction monitoring (MRM). The external standard method was used for the quantification. The calibration graphs were linear in their concentration ranges with the correlation coefficients more than 0.990. The limits of detection (LOD, S/N=3) were between 0.02-1.0 μ g/L. The recoveries for all the drugs in health foods were 82.3%-114.8% with the relative standard deviations less than 14.1% at three spiked levels. Thirteen kinds of health foods were tested, in which meprobamate and oxazepan were found in one sample separately, and zaleplon was found in two samples. The method is specific, sensitive, easy and quick and suitable for the confirmation and quantification of the 23 sedative drugs in health foods.
- high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS),
- sedative drugs,
- health foods
1. [1]
  [1] Morin C M, Espie C A. Insomnia-A Clinical Guide to Assessment and Treatment. New York: Kluwer Academic Publishers, 2004
2. [2]
  [2] Caplan J P, Epstein L A, Quinn D K, et al. Neuropsychol Rev, 2007, 17: 363
3. [3]
  [3] Sauve E N, Langφdegård M, Ekeberg D, et al. J Chromatogr B, 2012, 883/884: 177
4. [4]
  [4] Borrey D, Meyer E, Lambert W, et al. J Chromatogr B, 2001, 765: 187
5. [5]
  [5] Irving R C, Dickson S J. Forensic Sci Int, 2007, 166: 58
6. [6]
  [6] Lund H M E, Φ iestad E L, Gjerde H, et al. J Chromatogr B, 2011, 879: 3367
7. [7]
  [7] Su H B, Li B K, Wu P F, et al. Journal of Qiqihar University of Medicine (苏海滨, 李百舸, 吴鹏飞, 等. 齐齐哈尔医学院学报), 2011, 32(4): 514
8. [8]
  [8] Zhang J, Shao B, Yin J, et al. J Chromatogr B, 2009, 877: 1915
9. [9]
  [9] Yao Y, Li H Y, Zhang Q M, et al. Chinese Pharmaceutical Journal (姚羽, 李慧义, 张启明, 等. 中国药学杂志), 2007, 42(3): 224
10. [10]
  [10] Jin P F, Wang Z H, Wang W F, et al. Chinese Pharmaceutical Journal (金鹏飞, 王志宏, 王伟夫, 等. 中国药学杂志), 2012, 47(6): 462
11. [11]
  [11] Xiao L H, Liu J J, Guan X Y, et al. China Modern Medicine (肖丽和, 刘吉金, 关潇滢, 等. 中国当代医药), 2009, 16(14): 15
12. [12]
  [12] Cooper J, Delahaut P, Fodey T L, et al. Analyst, 2004, 129(2): 169
13. [13]
  [13] Mercolini L, Bugamelli F, Kenndler E, et al. J Chromatogr B, 2007, 846(1/2): 273
14. [14]
  [14] Mercolini L, Mandrioli R, Iannello C, et al. Talanta, 2009, 80(1): 279
15. [15]
  [15] Nishioka R. J Chromatogr B, 1996, 681: 401
16. [16]
  [16] Krogh M, Grefslie H, Rasmussen K E, et al. J Chromatogr B, 1997, 689(2): 357
17. [17]
  [17] Gunnar T, Ariniemi K, Lillsunde P. J Chromatogr B, 2005, 818: 175
18. [18]
  [18] Rust K Y, Baumgartner M R, Meggiolaro N, et al. Forensic Sci Int, 2012, 215: 64
19. [19]
  [19] Lendoiro E, Quintela O', Castro A D, et al. Forensic Sci Int, 2012, 217: 207
20. [20]
  [20] Xu C B, Suo R, Zhang F, et al. Chinese Journal of Chromatography (许成保, 锁然, 张峰, 等. 色谱), 2012, 30(5): 457
21. [21]
  [21] Ishida T, Kudo K, Hayashida M, et al. J Chromatogr B, 2009, 877: 2652
22. [22]
  [22] Chen X Q, Jin Y Y, Tang G. New Pharmacology. 17th ed. Beijing: People's Medical Publishing House (陈新谦, 金有豫, 汤光. 新编药物学. 17版. 北京: 人民卫生出版社), 2011
23. [23]
  [23] Greulich K, Alder L. Anal Bioanal Chem, 2008, 391: 183
24. [24]
  [24] Gilbert-López B, García-Reyes J F, Lozano A, et al. J Chromatogr A, 2010, 1217: 6022
25. [25]
  [25] GB/T 27404-2008
1. [1]
  Haiyuan Wang , Xiaoning Jin , Yajing Sun , Zhen Zhang , Wentao Zhao , Yi Li . Practical Exploration of High-Performance Liquid Chromatography Experiment Teaching Reform Empowered by Artificial Intelligence. University Chemistry, 2026, 41(4): 45-51. doi: 10.12461/PKU.DXHX202505076
2. [2]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
3. [3]
  Siwei Hou , Yaxin Niu , Guanglu Zhang , Yanmei Yang , Xu Wang , Zhenzhen Chen . Application of Solid-Phase Microextraction and Mass Spectrometry in Environmental Detection. University Chemistry, 2026, 41(3): 297-306. doi: 10.12461/PKU.DXHX202504078
4. [4]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
5. [5]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
6. [6]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . Sulfide Solid Electrolyte Synthesized by Liquid Phase Approach and Application in All-Solid-State Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(1): 100004-0. doi: 10.3866/PKU.WHXB202309019
7. [7]
  Haifeng Liu , Yong Xiao , Teng Yuan , Bimin Lin , Yizhen Wang , Hui Zeng . Exploration of Safety Facility Configuration in University Chemical Depot. University Chemistry, 2024, 39(10): 182-188. doi: 10.3866/PKU.DXHX202401036
8. [8]
  Jianguo Wang . Patriotic Dedication of Professor Li Zheng-Ming: Reflections from the Establishment of the Li Zheng-Ming Scholarship. University Chemistry, 2025, 40(11): 391-396. doi: 10.12461/PKU.DXHX202412089
9. [9]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
10. [10]
  Chongjing Liu , Yujian Xia , Pengjun Zhang , Shiqiang Wei , Dengfeng Cao , Beibei Sheng , Yongheng Chu , Shuangming Chen , Li Song , Xiaosong Liu . Understanding Solid-Gas and Solid-Liquid Interfaces through Near Ambient Pressure X-Ray Photoelectron Spectroscopy. Acta Physico-Chimica Sinica, 2025, 41(2): 100013-0. doi: 10.3866/PKU.WHXB202309036
11. [11]
  Ke Qiu , Fengmei Wang , Mochou Liao , Kerun Zhu , Jiawei Chen , Wei Zhang , Yongyao Xia , Xiaoli Dong , Fei Wang . A Fumed SiO₂-based Composite Hydrogel Polymer Electrolyte for Near-Neutral Zinc-Air Batteries. Acta Physico-Chimica Sinica, 2024, 40(3): 2304036-0. doi: 10.3866/PKU.WHXB202304036
12. [12]
  Simin Fang , Hong Wu , Sizhe Sheng , Lingling Li , Yuxi Wang , Hongchun Li , Jun Jiang . The Food Kingdom Lecture Series: The Science behind Color. University Chemistry, 2024, 39(9): 177-182. doi: 10.12461/PKU.DXHX202402012
13. [13]
  Linghua Chen . 基于双联动“三学”模式的食品专业分析化学教学改革. University Chemistry, 2025, 40(8): 78-91. doi: 10.12461/PKU.DXHX202409095
14. [14]
  Shunü Peng , Huamin Li , Zhaobin Chen , Yiru Wang . Simultaneous Application of Multiple Quantitative Analysis Methods in Gas Chromatography for the Determination of Active Ingredients in Traditional Chinese Medicine Preparations. University Chemistry, 2025, 40(10): 243-249. doi: 10.12461/PKU.DXHX202412043
15. [15]
  Yu Peng , Jiawei Chen , Yue Yin , Yongjie Cao , Mochou Liao , Congxiao Wang , Xiaoli Dong , Yongyao Xia . Tailored cathode electrolyte interphase via ethylene carbonate-free electrolytes enabling stable and wide-temperature operation of high-voltage LiCoO₂. Acta Physico-Chimica Sinica, 2025, 41(8): 100087-0. doi: 10.1016/j.actphy.2025.100087
16. [16]
  Yajie Li , Bin Chen , Yiping Wang , Hui Xing , Wei Zhao , Geng Zhang , Siqi Shi . Inhibiting Dendrite Growth by Customizing Electrolyte or Separator to Achieve Anisotropic Lithium-Ion Transport: A Phase-Field Study. Acta Physico-Chimica Sinica, 2024, 40(3): 2305053-0. doi: 10.3866/PKU.WHXB202305053
17. [17]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
18. [18]
  Tong Wang , Liangyu Hu , Shiqi Chen , Xinqiang Fu , Rui Wang , Kun Li , Shuangyan Huan . Determination of Benzenediol Isomers in Cosmetics Using High-Performance Liquid Chromatography Empowered by “Mathematical Separation”. University Chemistry, 2026, 41(1): 9-19. doi: 10.12461/PKU.DXHX202503128
19. [19]
  Wei Li , Jinfan Xu , Yongjun Zhang , Ying Guan . 共价有机框架整体材料的制备及食品安全非靶向筛查应用——推荐一个仪器分析综合化学实验. University Chemistry, 2025, 40(6): 276-285. doi: 10.12461/PKU.DXHX202406013
20. [20]
  Lin Wang , Xiaozhou Li , Haishuang Zhao , Yutang Wang , Jianguo Wang , Junru Wang . Design and Practice of New-Form Course Resources for Physical Chemistry in Agricultural and Forestry Universities: A Case Study of Teaching in Food Science and Engineering Major. University Chemistry, 2026, 41(2): 146-153. doi: 10.12461/PKU.DXHX202412149

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(679)
HTML views(31)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142