Citation: ZHANG Xiaohui, WANG Rong, XIE Hua, YIN Qiang, LI Xiaoyun, JIA Zhengping, WU Xiaoyu, ZHANG Juanhong, LI Wenbin. Online enrichment ability of restricted-access column coupled with high performance liquid chromatography by column switching technique for benazepril hydrochloride[J]. Chinese Journal of Chromatography, ;2013, 31(5): 451-455. doi: 10.3724/SP.J.1123.2012.11021 shu

Online enrichment ability of restricted-access column coupled with high performance liquid chromatography by column switching technique for benazepril hydrochloride

ZHANG Xiaohui ¹ ,
WANG Rong ^1,2,, ,
XIE Hua ¹ ,
YIN Qiang ¹ ,
LI Xiaoyun ¹ ,
JIA Zhengping ^1,2,, ,
WU Xiaoyu ¹ ,
ZHANG Juanhong ¹ ,
LI Wenbin ¹

1.
1. PLA Key Laboratory of the Plateau of the Environmental Damage Control, Lanzhou General Hospital, Lanzhou Command, Lanzhou 730050, China;

Corresponding author: WANG Rong, JIA Zhengping,
Received Date: 28 November 2012
Available Online: 14 January 2013
Fund Project: 国家科技部重大资助项目(2008ZXJ09014-010) (2008ZXJ09014-010)全军"十二五"医药科研重点项目(BWS12J012) (BWS12J012)全军"十二五"医药科研面上项目(CWS11C231). (CWS11C231)

The online enrichment ability of the restricted-access media (RAM) column coupled with high performance liquid chromatography by column switching technique for benazepril hydrochloride in plasma was studied. The RAM-HPLC system consisted of an RAM column as enrichment column and a C18 column as analytical column coupled via the column switching technique. The effects of the injection volume on the peak area and the systematic pressure were studied. When the injection volume was less than 100 μL, the peak area increased with the increase of the injection volume. However, when the injection volume was more than 80 μL, the pressure of whole system increased obviously. In order to protect the whole system, 80 μL was chosen as the maximum injection volume. The peak areas of ordinary injection and the large volume injection showed a good linear relationship. The enrichment ability of RAM-HPLC system was satisfactory. The system was successfully used for the separation and detection of the trace benazepril hydrochloride in rat plasma after its administration. The sensitivity of HPLC can be improved by RAM pre-enrichment. It is a simple and economic measurement method.
- restricted-access media (RAM),
- high-performance liquid chromatography (HPLC),
- online enrichment,
- direct injection,
- benazepril hydrochloride,
- serum
1. [1]
  [1] Hu X G, Luo Y, Zhou Q X, et al. Chinese Journal of Analytical Chemistry (胡献刚, 罗义, 周启星, 等. 分析化学), 2008, 36(9): 1162
2. [2]
  [2] Xu W J, Su S F, Jiang P, et al. J Chromatogr A, 2010, 1217: 7198
3. [3]
  [3] Gil G, Brennan J, Throckmorton D J, et al. J Chromatogr B, 2011, 879: 1112
4. [4]
  [4] Wang Y, Wang Y X, Chen L, et al. J Magn Magn Mater, 2012, 324: 410
5. [5]
  [5] Kishida K, Furusawa N. J Chromatogr A, 2004, 1028(1): 175
6. [6]
  [6] Lamprecht G, Stoschitzky K. J Chromatogr B, 2009, 877(29): 3489
7. [7]
  [7] Guo Z Q, Ma J, Jia Z P, et al. Chinese Journal of Pharmaceutical Analysis (郭志强, 马骏, 贾正平, 等. 药物分析杂志), 2006, 26(6): 758
8. [8]
  [8] Gasparrini F, Cancelliere G, Ciogli A, et al. J Chromatogr A, 2008, 1191: 205
9. [9]
  [9] Fan P C, Jia Z P, Ma J, et al. Chinese Journal of Pharmaceutical Analysis (樊鹏程, 贾正平, 马骏, 等. 药物分析杂志), 2008, 28(2): 329
10. [10]
  [10] Yang P, Wang R, Ma J, et al. Analytical Instrumentation (杨沛, 王荣, 马骏, 等. 分析仪器), 2011(3): 1
11. [11]
  [11] Guo Z Q, Ma J, Jia Z P, et al. Chinese Journal of Pharmaceutical Analysis (郭志强, 马骏, 贾正平, 等. 药物分析杂志), 2006, 26(8): 1071
12. [12]
  [12] Ma J, Jia Z P, Wang R, et al. Chinese Journal of Pharmaceutical Analysis (马骏, 贾正平, 王荣, 等. 药物分析杂志), 2005, 25(12): 1420
13. [13]
  [13] Wu X Y, Wang R, Xie H, et al. Chinese Journal of Chromatography (武晓玉, 王荣, 谢华, 等. 色谱), 2011, 29(12): 1205
14. [14]
  [14] Hilton J, Yokoi F, Dannals R F, et al. Nucl Med Biol, 2000, 27(6): 627
15. [15]
  [15] Wang X D, Chan E, Chen X, et al. J Pharm Biomed Anal, 2007, 44: 224
16. [16]
  [16] Wang R, Jia Z P, Xie H, et al. China Patent (王荣, 贾正平, 谢华, 等. 中国专利), 2012100351314. 2012-02-16
17. [17]
  [17] Wu X Y, Wang R, Xie H, et al. Chinese Journal of Chromatography (武晓玉, 王荣, 谢华, 等. 色谱), 2012, 30(8): 810
1. [1]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Exploration and Practice of Online and Offline Hybrid Teaching Mode in High-Performance Liquid Chromatography Experiment. University Chemistry, 2025, 40(11): 100-107. doi: 10.12461/PKU.DXHX202412133
2. [2]
  Haiyuan Wang , Xiaoning Jin , Yajing Sun , Zhen Zhang , Wentao Zhao , Yi Li . Practical Exploration of High-Performance Liquid Chromatography Experiment Teaching Reform Empowered by Artificial Intelligence. University Chemistry, 2026, 41(4): 45-51. doi: 10.12461/PKU.DXHX202505076
3. [3]
  Tong Wang , Liangyu Hu , Shiqi Chen , Xinqiang Fu , Rui Wang , Kun Li , Shuangyan Huan . Determination of Benzenediol Isomers in Cosmetics Using High-Performance Liquid Chromatography Empowered by “Mathematical Separation”. University Chemistry, 2026, 41(1): 9-19. doi: 10.12461/PKU.DXHX202503128
4. [4]
  Fan Wu , Wenchang Tian , Jin Liu , Qiuting Zhang , YanHui Zhong , Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031
5. [5]
  Siming Bian , Sijie Luo , Junjie Ou . Application of van Deemter Equation in Instrumental Analysis Teaching: A New Type of Core-Shell Stationary Phase. University Chemistry, 2025, 40(3): 381-386. doi: 10.12461/PKU.DXHX202406087
6. [6]
  Gengjia Chen , Junjie Ou . Application of the van Deemter Equation in Instrumental Analysis Teaching: A Case of Organic Polymer Monolithic Columns. University Chemistry, 2025, 40(11): 362-368. doi: 10.12461/PKU.DXHX202502003
7. [7]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
8. [8]
  Qiuting Zhang , Fan Wu , Jin Liu , Zian Lin . Chromatographic Stationary Phase and Chiral Separation Using Frame Materials. University Chemistry, 2025, 40(4): 291-298. doi: 10.12461/PKU.DXHX202405174
9. [9]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
10. [10]
  Ruiqing LIU , Wenxiu LIU , Kun XIE , Yiran LIU , Hui CHENG , Xiaoyu WANG , Chenxu TIAN , Xiujing LIN , Xiaomiao FENG . Three-dimensional porous titanium nitride as a highly efficient sulfur host. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 867-876. doi: 10.11862/CJIC.20230441
11. [11]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
12. [12]
  Shunü Peng , Huamin Li , Zhaobin Chen , Yiru Wang . Simultaneous Application of Multiple Quantitative Analysis Methods in Gas Chromatography for the Determination of Active Ingredients in Traditional Chinese Medicine Preparations. University Chemistry, 2025, 40(10): 243-249. doi: 10.12461/PKU.DXHX202412043
13. [13]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . Sulfide Solid Electrolyte Synthesized by Liquid Phase Approach and Application in All-Solid-State Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(1): 100004-0. doi: 10.3866/PKU.WHXB202309019
14. [14]
  Yajie Li , Bin Chen , Yiping Wang , Hui Xing , Wei Zhao , Geng Zhang , Siqi Shi . Inhibiting Dendrite Growth by Customizing Electrolyte or Separator to Achieve Anisotropic Lithium-Ion Transport: A Phase-Field Study. Acta Physico-Chimica Sinica, 2024, 40(3): 2305053-0. doi: 10.3866/PKU.WHXB202305053
15. [15]
  Jiying Liu , Zehua Li , Wenjing Zhang , Donghui Wei . Molecular Orbital and Nucleus-Independent Chemical Shift Calculations for C₆H₆ and B₁₂H₁₂^2-: A Computational Chemistry Experiment. University Chemistry, 2025, 40(3): 186-192. doi: 10.12461/PKU.DXHX202406085
16. [16]
  Tianlong Zhang , Rongling Zhang , Hongsheng Tang , Yan Li , Hua Li . Online Monitoring and Mechanistic Analysis of 3,5-diamino-1,2,4-triazole (DAT) Synthesis via Raman Spectroscopy: A Recommendation for a Comprehensive Instrumental Analysis Experiment. University Chemistry, 2024, 39(6): 303-311. doi: 10.3866/PKU.DXHX202312006
17. [17]
  Lijuan Wang , Yuping Ning , Jian Li , Sha Luo , Xiongfei Luo , Ruiwen Wang . Enhancing the Advanced Nature of Natural Product Chemistry Laboratory Courses with New Research Findings: A Case Study of the Application of Berberine Hydrochloride in Photodynamic Antimicrobial Films. University Chemistry, 2024, 39(11): 241-250. doi: 10.12461/PKU.DXHX202403017
18. [18]
  Binbin Liu , Yang Chen , Tianci Jia , Chen Chen , Zhanghao Wu , Yuhui Liu , Yuhang Zhai , Tianshu Ma , Changlei Wang . Hydroxyl-functionalized molecular engineering mitigates 2D phase barriers for efficient wide-bandgap and all-perovskite tandem solar cells. Acta Physico-Chimica Sinica, 2026, 42(1): 100128-0. doi: 10.1016/j.actphy.2025.100128
19. [19]
  Jiayao Wang , Guixu Pan , Ning Wang , Shihan Wang , Yaolin Zhu , Yunfeng Li . Preparation of donor-π-acceptor type graphitic carbon nitride photocatalytic systems via molecular level regulation for high-efficient H₂O₂ production. Acta Physico-Chimica Sinica, 2025, 41(12): 100168-0. doi: 10.1016/j.actphy.2025.100168
20. [20]
  Yiming Liang , Ziyan Pan , Kin Shing Chan . One Drink, Two Tears in the Central Nervous System: The Perils of Disulfiram-Like Reactions. University Chemistry, 2025, 40(4): 322-325. doi: 10.12461/PKU.DXHX202406016

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(1022)
HTML views(111)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142