Citation: ZENG Kai, LIU Zhirong, NING Yajun, LI Jinlin, TANG Jun, ZHANG Min. Simultaneous determination of melamine and creatinine in milk and milk products by gas chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, ;2013, 31(5): 477-480. doi: 10.3724/SP.J.1123.2012.11008 shu

Simultaneous determination of melamine and creatinine in milk and milk products by gas chromatography-mass spectrometry

ZENG Kai ^1,, ,
LIU Zhirong ² ,
NING Yajun ¹ ,
LI Jinlin ¹ ,
TANG Jun ¹ ,
ZHANG Min ¹

1.
1. Nanchang Food Quality Health Safety Supervision and Inspection Center, Nanchang 330012, China;

Corresponding author: ZENG Kai,
Received Date: 19 November 2012
Available Online: 23 January 2013
Fund Project: 江西省质量技术监督局科技项目(GZJK201102). (GZJK201102)

A method established for determining melamine and creatinine in milk and milk products. The samples were extracted with 1% trichloracetic acid solution. The extract was cleaned-up by using a mixed-mode cation ion exchange SPE column and evaporated to dryness under nitrogen. The residue was derived by bis(trimethylsilyl)trifluoroacetamide-chlorotrimethylsilane (BSTFA-TMCS), then determined by gas chromatography-mass spectrometry (GC-MS) in selected ion monitoring (SIM) mode. Melamine and creatinine had good linearity in the range of 0.1-50 mg/L with the correlation coefficients higher than 0.99, and the limits of quantification were 0.10 mg/kg and 0.20 mg/kg, respectively. The recoveries of melamine and creatinine were 80.7%-116.8% and 77.6%-107.5% with RSD no more than 9.4% and 8.5% in the spiked range of 10-100 mg/kg and 0.1-5.0 mg/kg, respectively. The method is accurate, sensitive, and suitable for the determination of creatinine in milk and milk products.
- gas chromatography-mass spectrometry (GC-MS),
- melamine,
- creatinine,
- milk,
- milk products
1. [1]
  [1] Zhang D L, Kan Y D. International Journal of Laboratory Medicine (张东玲, 阚耀东. 中国卫生检验杂志), 2006, 27(6): 521
2. [2]
  [2] GB 5009.5-2010
3. [3]
  [3] GB/T 21704-2008
4. [4]
  [4] GB/T 22388-2008
5. [5]
  [5] GB/T 22288-2008
6. [6]
  [6] Li F G, Yao W Q, Su M, et al. Chinese Journal of Chromatography (李锋格, 姚伟琴, 苏敏, 等. 色谱), 2009, 27(2): 233
7. [7]
  [7] Li F G, Yao W Q, Tian Y H, et al. Chinese Journal of Chromatography (李锋格, 姚伟琴, 田延河, 等. 色谱), 2010, 28(3): 319
8. [8]
  [8] WS/T 98-1996
9. [9]
  [9] Lü Y P, Zhou Y L. Chinese Journal of Analysis Laboratory (吕亚萍, 周永列. 分析试验室), 2006, 25(2): 26
10. [10]
  [10] Li X W, Jiang J, Zhu H, et al. Chinese Journal of Analysis Laboratory (黎晓维, 姜静, 祝贺, 等. 分析试验室), 2007, 26(6): 69
11. [11]
  [11] Dai X H, Fang X, Quan C, et al. Journal of Instrumental Analysis (戴新华, 方向, 全灿, 等. 分析测试学报), 2007, 26(5): 763
12. [12]
  [12] Shi L B, Lin L S. Chinese Journal of Medical Laboratory Technology (石凌波, 林龙顺. 中华检验医学杂志), 2001, 24(2): 102
13. [13]
  [13] Tsikas D, Wolf A, Mitschkea A, et al. J Chromatogr B, 2010, 878(27): 2582
14. [14]
  [14] MacNeil L, Hill L, MacDonald D, et al. J Chromatogr B, 2005, 827(2): 210
15. [15]
  [15] Stokes P, O'Connor G. J Chromatogr B, 2003, 794(1): 125
16. [16]
  [16] Guan Y G, Ding X R, Zheng L, et al. Science and Technology of Food Industry (管有根, 丁献荣, 郑林, 等. 食品工业科技), 2011, 32(5): 392
17. [17]
  [17] Mclafferty F W. Interpretation of Mass Spectra. 3rd ed. Wang G H, Jiang L F, Wang C H, transl. Beijing: Chemical Industry Press (麦克拉弗蒂F W .质谱解析. 3版. 王光辉, 姜龙飞, 王聪慧, 译. 北京: 化学工业出版社), 1999
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