Citation: XIAO Tian-Peng, ZHENG Xiao-Feng, LV Ji-Hong, GUO Ze-Feng, GAO Feng, LIU Chun-Feng, LI Qi. Detection of Pyrazine Compounds in Cocoa Wort by Gas Chromatography with Headspace Solid Phase Micro-Extraction[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(10): 1589-1592. doi: 10.3724/SP.J.1096.2012.20324 shu

Detection of Pyrazine Compounds in Cocoa Wort by Gas Chromatography with Headspace Solid Phase Micro-Extraction

XIAO Tian-Peng ^1,2 ,
ZHENG Xiao-Feng ³ ,
LV Ji-Hong ³ ,
GUO Ze-Feng ³ ,
GAO Feng ³ ,
LIU Chun-Feng ^1,2 ,
LI Qi ^1,2,,

1.
¹ The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;

Corresponding author: LI Qi,
Received Date: 28 March 2012
Available Online: 7 June 2012
Fund Project: 本文系国家"十一五"支撑重点项目(No.2008BAI63B06)资助 (No.2008BAI63B06)

A headspace solid phase microextraction (HS-SPME), followed by gas chromatographic method was established for the detection of three pyrazine compounds (2,5-dimethyl pyrazine, 2,3,5-trimethyl pyrazine and 2,3,5,6-tetramethyl pyrazine) in cocoa wort. Equilibrium temperature and equilibrium time were optimized by using different SPME heads. According to the results of the optimization experiments, the following conclusion can be drawn:The cocoa wort sample was efficiently extracted under 40℃ for 40 min with 75 μm CAR/PDMS SPME head. The method had linear response within 1-500 mg/L, and the detection limits (S/N＝3) were below 0.023 μg/L. The relative standard deviations were from 3.6%-6.4% and the recoveries were from 95.4% to 102.7%. This method is characterized by rapidly, high sensitivity, good linearity and repeatability for the analysis of cocoa wort samples.
- Headspace solid phase microextraction,
- Pyrazine compounds,
- Gas chromatography,
- Cocoa wort
1. [1]
  1 Sylvie D, Javier M, Albert Z, Antonio T, David M. Talanta, 2008, 74(5):1168-1169
2. [2]
  2 Yue L, Yun F, Song Z, Chang R L, Jian G M, Fang Z. Food Composition and Analysis, 2012, 25(1):21-23
3. [3]
  3 Emmanuel O, Alistair P, Mark F, Angela R. Food Science and Nutrition, 2008, 48(9):48-49
4. [4]
  4 Nazaruddin R, Osman H, Mamot S, Mamot S, Wahid S, Noraini I J. Food Processing and Preservation, 2006, 30(3):280-282
5. [5]
  5 Christine C, Delphine C, Caroline O, Sonia C. Agricultural and Food Chemistry, 2002, 50(8):2386-2389
6. [6]
  6 de Britoa E S, Narainb N. Food Quality and Preference, 2003, 14(2003):219-221
7. [7]
  7 Bonveh J S. European Food Research and Technology, 2005, 221(2005):19-24
8. [8]
  8 Jinap M S, Jamilah B, Nazamid S. European Food Research and Technology, 2004, 85(2004):74-77
9. [9]
  9 Fabienne L, Dominique C, Loǐc Cirou, Peter B. Agricultural and Food Chemistry, 2002, 50(12):3527-3529
10. [10]
  10 SHENG Hong-Lin, XIANG Neng-Jun, XU Yong,GAO Qian, MIAO Ming-Ming, LU She-Ming, TANG Jian-Guo. Chinese Journal of Analysis Laboratory, 2009, 28(4):88
11. [11]
  沈宏林, 向能军, 许永, 高茜, 缪明明, 陆舍铭, 汤建国. 分析试验室, 2009, 28(4):88
12. [12]
  11 MA Kang, ZHANG Jin-Na, HE Ya-Juan, ZHAO Min, GONG Ai-Jun. Chinese J. Anal. Chem., 2011, 39(12):1823-1824
13. [13]
  马康, 张金娜, 何雅娟, 赵敏, 弓爱君. 分析化学, 2011, 39(12):1823-1824
14. [14]
  12 FENG Lin. Physical Testing and Chemical Analysis Part B:Chemical Analgsis, 2011, 47(1):86
15. [15]
  冯琳. 理化检验-化学分册, 2011, 47(1):86
16. [16]
  13 WANG Guan-Nan, TANG Hua, CHEN Da-Zhou, FENG Jie, LI Lei. Chinese Journal of Chromatography, 2012, 30(2):135
17. [17]
  王冠男, 汤桦, 陈大周, 冯洁, 李蕾. 色谱, 2012, 30(2):135
18. [18]
  14 Xing L S, Jin H L, Ling X C, Zong W C, Chun Y L, Hai L P, Hua X. Brazilian Chemical Society, 2012, 23 (1):132
19. [19]
  15 ZHANG Yan-Hong, FAN Wen-Lai, XU Yan, WANG Lin-Xiang. Chinese J. Analysis Laboratory, 2008, 27(6):39-40
20. [20]
  张艳红, 范文来, 徐岩, 王林祥. 分析试验室, 2008, 27(6):39-40
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