Citation: DU Min, YE Xiong-Ying, FENG Jin-Yang, MA Zeng-Shuai, ZHOU Zhao-Ying. An Enrichment Method for Bio-samples Based on Circular Injection on a Microfluidic Chip[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(11): 1668-1673. doi: 10.3724/SP.J.1096.2012.20203 shu

An Enrichment Method for Bio-samples Based on Circular Injection on a Microfluidic Chip

1.
State Key Laboratory of Transducer Technology, Department of Precision Instruments & Mechanology, Tsinghua University, Beijing 100084, China

Corresponding author: YE Xiong-Ying,
Received Date: 29 February 2012
Available Online: 25 May 2012
Fund Project: 本文系国家重点基础研究发展计划(No. 2007CB310504) (No. 2007CB310504)国家自然科学基金(No.50730009)资助项目 (No.50730009)

A microfluidic chip based circular injection method for bio-samples was presented. The microfluidic chip with an annular peristaltic micropump and electromagnetic microvalves was fabricated using PDMS (polydimethylsiloxane) soft lithography process. The chip was then integrated with an immune probe chip immobilized with rabbit IgG, and realized the circular injection of Goat-anti-rabbit IgG (FITC labeled) solution and sample enrichment. Higher fluorescence intensity was obtained using circular injection than that using static adsorption, under different sample concentrations and 3 min binding time. By increasing the pumping speed from 130 μL/min to 300 μL/min, the immune binding speed with 50 mg/L sample concentration was accelerated evidently, which meant the detection limit at a given time could be lower, but for 200 mg/L sample concentration, the effect was weaker. Compared to the continuous injection mode with about 1.8 mL of sample consumption, only 7 μL sample was needed to realize the coincident results with the circular injection mode.
- Microfluidic,
- Micropump,
- Circulation,
- Sample injection,
- Enrichment
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沈阳化工大学材料科学与工程学院沈阳 110142