Citation: MA Kang, JIANG Xiao-Xiong, ZHAO Min, CAI Ye-Qiang. Simultaneous Determination of 20 Food Additives in Drinks by High Performance Liquid Chromatography Coupled with Photo-diode Array Detector[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(11): 1661-1667. doi: 10.3724/SP.J.1096.2012.20176 shu

Simultaneous Determination of 20 Food Additives in Drinks by High Performance Liquid Chromatography Coupled with Photo-diode Array Detector

1.
¹ Division of Chemical Metrology and Analytical Science, National Institute of Metrology of China, Beijing 100013, China;

Corresponding author: MA Kang,
Received Date: 24 April 2012
Available Online: 21 June 2012
Fund Project: 本文系国家质检总局食品安全专项(No. ASPAQ1010) (No. ASPAQ1010)中国计量科学研究院基础科研费(No. AKY1216)资助 (No. AKY1216)

An efficient and accurate analytical method was developed for the simultaneous determination of 20 synthetic food additives, including acesulfame potassium, benzoic acid, sorbic acid, sodium saccharin, tartrazine, amaranthus red, ponceau 4R, caffeine, sunset yellow, aspartame, allure red, brilliant blue, erythrosine, methyl paraben, ethyl paraben, isopropyl paraben, n-propyl paraben, isobutyl paraben, n-butyl paraben and n-heptyl paraben, by high performance liquid chromatography (HPLC) coupled with photodiode array detector(PDA). A C₁₈ stationary phase was used and the mobile phase contained an acetonitrile-methanol (10:90, V/V) mixture and 0.1 mol/L ammonium acetate buffer solution at pH 7.2. Successful separation was obtained for all the compounds within 50 min at 30℃ by using gradient elution. The absorbance of 20 compounds behaved linearly with their concentrations ranged from 0.10 mg/L to 300 mg/L with correlation coefficients more than 0.998. The limits of detection of these compounds were from 0.005 mg/L to 0.110 mg/L. The recoveries were from 91.4% to 100.5% at spiked levels of 10-100 mg/L, The precision (n=6) was range from 0.6% to 4.0%. The method has been used in the determination of 16 food additives in various drinks.
- Food additives,
- Liquid chromatography,
- Photodiode array detection,
- Simultaneous determination,
- Drinks
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沈阳化工大学材料科学与工程学院沈阳 110142