Citation: DU Bi-Bai, XU Yang, Huang Yun-Hong, Kang Min. Determination of Sulfonamides in Feeds by High Performance Liquid Chromatography with Molecularly Imprinted Solid Phase Extraction[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(12): 1871-1876. doi: 10.3724/SP.J.1096.2012.20161 shu

Determination of Sulfonamides in Feeds by High Performance Liquid Chromatography with Molecularly Imprinted Solid Phase Extraction

DU Bi-Bai ¹ ,
XU Yang ^, ,
Huang Yun-Hong ^1,2 ,
Kang Min ¹

1.
¹ State Key Laboratory of Food Science and Technology, Sino-Germany Joint Research Institute, Nanchang University, Nanchang 330047, China;

Corresponding author: XU Yang,
Received Date: 22 February 2012
Available Online: 9 July 2012
Fund Project: 本文系食品科学与技术国家重点实验室自由探索课题(No.SKLF-TS-200815)资助 (No.SKLF-TS-200815)

A method was developed for monitoring five sulfonamides (SAs) in feeds by using molecularly imprinted polymers (MIPs) as selective solid-phase extraction sorbents coupled with high performance liquid chromatography. The MIPs were firstly synthesized by ultraviolet initiated using sulfadiazine as template. Then, the molecularly printed solid phase extraction (MISPE) column was prepared, and the MIPs were used as adsorbent. Meanwhile, the procedures of loading, washing and eluting conditions were optimized. Under the optimum conditions, the detection limit range of five SAs was 0.14-0.23 mg/kg and the quantification limit range was 0.38-0.47 mg/kg. The average recoveries were in the range of 72.1%-89.3% with a batch and batch sampling relative standard deviations less than 7.9% and 9.2%, respectively. It showed that the MISPE column decreased the matrix effect and had a better selectivity and a lower limit of quantification than Alumina B column.
- Molecularly imprinted polymer,
- Solid-phase extraction,
- Sulfonamides,
- Feed,
- High performance liquid chromatography
1. [1]
  1 Heuer H, Solehati Q, Zimmerling U, Kleineidam K, Schloter M, Müller T, Focks A, Thiele-Bruhn S, Smalla K. Appl. Environ. Microb., 2011, 77(7): 2527-2530
2. [2]
  2 Willmott A, McCombie A, Rossleigh M. Intern. Med. J., 2011, 41(Suppl. 3): 25-26
3. [3]
  3 Wang S, Zhang H Y, Wang L, Duan Z J, Kennedy I. Food Addit. Contam., 2006, 23(4): 362-384
4. [4]
  4 LIU Gui-Hua, HAN De-Man, Liang Hua-Ding, TANG Shou-Wan, PAN Fu-You, YAN Rui-Qiang. Chinese J. Anal. Chem., 2012, 40(3): 432-436
5. [5]
  刘贵花, 韩德满, 梁华定, 唐守万, 潘富友, 阎瑞强. 分析化学, 2012, 40(3): 432-436
6. [6]
  5 Blüthgen A H. Bull. Int. Dairy Feder., 2000, 356: 43-47
7. [7]
  6 Segato G, Benetti C, Angeletti R, Montesissa C, Biancotto G. Food Addit. Contam. A, 2011, 28(7): 860-868
8. [8]
  7 Albert K, Riter K L, Smallidge R L. J. Aoac Int , 2003, 86(4): 623-630
9. [9]
  8 Iammarino M, Palermo C, Nardiello D, Muscarella M. Chromatographia, 2011, 73(Suppl. 1): S75-S82
10. [10]
  9 Borràs S, Companyó R, Guiteras J. J. Agr. Food Chem., 2011, 59(10): 5240-5247
11. [11]
  10 Kumar P, Companyó R. Drug Test. Anal, 2012, 4(5): 368-375
12. [12]
  11 Croubels S, Wassink P, de Backer P. Anal. Chim. Acta, 2002, 473(1-2): 183-194
13. [13]
  12 Kantiani L, Farré M, Freixiedas J M G I, Barceló D. J. Chromatogr. A, 2010, 1217(26): 4247-4254
14. [14]
  13 Liu R Y, He P L, Li Z, Li R G. J. Chromatogr. Sci., 2011, 49(8): 640-646
15. [15]
  14 HU Xiao-Gang, LI Gong-Ke. Chinese J. Anal. Chem., 2006, 34(7): 1035-1041
16. [16]
  胡小刚, 李攻科. 分析化学, 2006, 34(7): 1035-1041
17. [17]
  15 YAN Hong-Yuan, YANG Geng-Liang. Chinese J. Chromatogr., 2011, 29(7): 572-579
18. [18]
  闫宏远, 杨更亮. 色谱, 2011, 29(7): 572-579
19. [19]
  16 Guo L Y, Jiang X M, Yang C L, Zhang H X. Anal. Bioanal. Chem., 2008, 391(6): 2291-2298
20. [20]
  17 He J X, Wang S, Fang G Z, Zhu H P, Zhang Y. J. Agric. Food Chem., 2008, 56(9): 2919-2925
21. [21]
  18 Vlatakis G, Andersson L I, Müller R, Mosbach K. Nature, 1993, 361(6413): 645-647
22. [22]
  19 Zhou X, He X W, Chen L X, Li W Y, Zhang Y K. Chinese J. Anal. Chem., 2009, 37(2): 174-180
23. [23]
  周雪, 何锡文, 陈朗星, 李文友, 张玉奎. 分析化学, 2009, 37(2): 174-180
24. [24]
  20 LIU Hui-Jun, XU Wei-Jian, LIU Yao-Chi, XIONG Yuan-Qin. Spectrosc. Spect. Anal., 2007, 27(8): 1596-1599
25. [25]
  刘慧君, 徐伟箭, 刘耀驰, 熊远钦. 光谱与光谱分析, 2007, 27(8): 1596-1599
26. [26]
  21 Zheng N, Li Y Z, Chang W B, Wang Z M, Li T J. Anal. Chim. Acta., 2002, 452(2): 277-283
27. [27]
  22 ZHANG Feng, XU Cheng-Bao, LAN Fang, WANG Xiu-Juan, YONG Wei , SUO Ruan, CHU Xiao-Gang , DING Fei , LV Quan-Fu . Chinese J. Anal. Chem., 2012, 40(1): 101-106
28. [28]
  张峰, 许成保, 蓝芳, 王秀娟, 雍炜, 锁然, 储晓刚, 丁菲, 吕泉福. 分析化学, 2012, 40(1): 101-106
29. [29]
  23 GB/T 19542-2007. Determination of Sulfonamides in Feeds-High Performance Liquid Chromatography. National Standards of the People's Republic of China
30. [30]
  GB/T 19542-2007. 饲料中磺胺类药物的测定-高效液相色谱法. 中华人民共和国国家标准
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