Citation: YU Hui-Juan, LI Bing, CAI You-Qiong, YE Fang-Ting, TAI Jian-Ming, HUI Yun-Hua, XU Jie, FENG Bing. Determination of Semicarbazide Content in Crustaceans by Liquid Chromatography Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(10): 1530-1535. doi: 10.3724/SP.J.1096.2012.20160 shu

Determination of Semicarbazide Content in Crustaceans by Liquid Chromatography Tandem Mass Spectrometry

YU Hui-Juan ^1,, ,
LI Bing ² ,
CAI You-Qiong ¹ ,
YE Fang-Ting ³ ,
TAI Jian-Ming ⁴ ,
HUI Yun-Hua ¹ ,
XU Jie ¹ ,
FENG Bing ¹

1.
¹ Fishery Products Quality Inspection and Test Center (Shanghai) of Ministry of Agiculture, Shanghai 200090, China;

Corresponding author: YU Hui-Juan,
Received Date: 22 February 2012
Available Online: 25 May 2012
Fund Project: 本文系农业部财政专项(No.2130109)资助 (No.2130109)

The content of semicarbazide in crustaceans had been determined by LC/MS/MS. Samples were hydrolyzed by hydrochloric acid and derivatized with 2-nitrobenzaidehyde. After extraction with ethyl acetate, chromatographic separation was achieved by HPLC and the target compound was detected by tandem mass spectrometry using internal standard as quantification method. The qualitative limit was 0.25 μg/kg(S/N＞3)and the quantitative limit was 0.5 μg/kg(S/N＞10). Crustacean species were from natural water and free market of agricultural products including oriental river prawn, giant tiger prawn, giant freshwater prawn, white-leg shrimp, red swamp crayfish, mud crab, dungeness crab, swimming crab, mitten crab and horse shoe crab. Concentrations of semicarbazide in shrimp and in crab were from not detected to 370.4 μg/kg and not detected to 87.5 μg/kg and concentrations of semicarbazide varied from tissues with highest in carapace and lowest in muscle. In the present study, the results revealed that semicarbazide was an endogenous substance existing in crustaceans.
- Semicarbazide,
- Endogenous substance,
- Crustacean aquatic products,
- Distribution characteristics
1. [1]
  1 Leitner A, Zollner P, Lindner W. J. Chromatogr. A, 2001, 939(1-2): 49-58
2. [2]
  2 Bulletin of Ministery of Agriculture of the People's Republic of China 235
3. [3]
  中华人民共和国农业部公告第235号
4. [4]
  3 Robert M C, Bob H, David E, Lynne C, Dermot F, Kennedy D G. Food Chemistry, Available online 25 November 2011
5. [5]
  4 Christof V P, Christ D, Mathieu W, Jan K, Patrick S, Carlos V P. Journal of Agricultural and Food Chemistry, 2011, 59: 2107-2112
6. [6]
  5 Saari L, Peltonen K. Food Additives and Contaminants, 2004, 21(9): 825-832
7. [7]
  6 CHEN Zhi-Feng, LI Cheng, SUN Li, YONG Wei. Food & Machinery, 2009, 25(2): 6-7
8. [8]
  陈志锋, 李成, 孙利, 雍炜. 食品与机械, 2009, 25(2): 6-7
9. [9]
  7 Samsonova J V, Douglas A J, Cooper K M, Kennedy D G, Elliott C T. Food and Chemical Toxicology, 2008, 46: 1548-1554
10. [10]
  8 WU Xiao-Jun, SHI Gao-Mao. Ocean and Fishery, 2007, 4: 25-26
11. [11]
  吴晓君, 施高茂. 海洋与渔业, 2007, 4: 25-26
12. [12]
  9 LI Chun-Feng, KANG Hai-Ning, YUE Zhen-Feng, YUAN Zong-Hui. Chinese Tournal of Veterinary Medicine, 2010, 46(2): 88-89
13. [13]
  李春风, 康海宁, 岳震峰, 袁宗辉. 中国兽医杂志, 2010, 46(2): 88-89
14. [14]
  10 Bulletin of Ministery of Agriculture of the People's Republic of China 783-1-2006 (农业部783号公告-1-2006)
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