Citation: ZHANG Xin-Zhong, LUO Feng-Jian, ZHANG Fen, LIU Guang-Ming, LOU Zheng-Yun, CHEN Zong-Mao. Residue Determination of Hexythiazox and Buprofezin in Tea, Marrow and Mango by Gas Chromatography Coupled with Tandem Mass Spectrometry after Dispersive Solid Phase Extraction Cleanup[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(11): 1686-1692. doi: 10.3724/SP.J.1096.2012.20093 shu

Residue Determination of Hexythiazox and Buprofezin in Tea, Marrow and Mango by Gas Chromatography Coupled with Tandem Mass Spectrometry after Dispersive Solid Phase Extraction Cleanup

ZHANG Xin-Zhong ^1,2,3 ,
LUO Feng-Jian ^1,2,3 ,
ZHANG Fen ⁴ ,
LIU Guang-Ming ^1,2,3 ,
LOU Zheng-Yun ^1,2,3 ,
CHEN Zong-Mao ^1,2,3,,

1.
¹ Research Center of Quality Safety for Agricultural Products, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;

Corresponding author: CHEN Zong-Mao,
Received Date: 8 February 2012
Available Online: 25 May 2012
Fund Project: 本文系国家茶叶产业技术体系建设专项资金资助项目(No.CARS-23) (No.CARS-23)中国农业科学院基本科研业务费专项(No.0032012026) (No.0032012026)农业部农药残留标准制(修)订CAC标准转化项目资助 (修)

A novel and sensitive method was developed and validated for the simultaneous determination of hexythiazox and buprofezin residue in tea, mango and marrow using gas chromatography coupled with tandem mass spectrometry. Target compounds were extracted by acetonitrile followed by dispersive solid phase extraction cleanup. The fragmentation pathway and matrix effect were investigated. Over the concentration in the range of 0.040-4.000 mg/L for buprofezin and 0.025-2.5 mg/L for hexythiazox in different matrix, the correlation coefficients (r) of the calibration curves were above 0.9910, and the limits of detection (LODs) were 0.005 mg/L and 0.010 mg/L for each other. The average recoveries of hexythiazox and buprofezin in different matrix at three spiked concentration levels ranged from 83.6% to 116.9% with relative standard deviations (RSDs) between 2.0% and 15.7% (n=5). The limits of quantitation (LOQs) of hexythiazox and buprofezin, were 0.010 mg/kg in green tea and 0.002 mg/kg in marrow and mango sample. Then, the proposed method was successfully applied to the routine market monitoring of mango and marrow in different regions in China, there was no hexythiazox and buprofezin residue detected in the all of samples (n=120); and it has also been applied to the residue analysis of buprofezin field test samples (n=20) in tea garden, the residues of buprofezin were from not detected to 3.9 mg/kg.
- Dispersive solid phase extraction,
- Gas chromatography tandem mass spectrometry,
- Tea,
- Mango,
- Marrow,
- Hexythiazox,
- Buprofezin
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