Citation: SUN Li, ZHAO Hai-Feng, GUO Hong-Hua, YANG Guang-Yuan, HE Cheng-Yan, SHI Qing-Hong, WEN Xue, WANG Xiao-Ting, ZHAO Li-Chun. Mass Spectrometric Analysis of Phosphorylation Modification in Talin from Human Colorectal Carcinoma Tissues[J]. Chinese Journal of Analytical Chemistry, ;2012, 40(10): 1500-1506. doi: 10.3724/SP.J.1096.2012.20027 shu

Mass Spectrometric Analysis of Phosphorylation Modification in Talin from Human Colorectal Carcinoma Tissues

1.
¹ China-Japan Union Hospital, Jilin University, Changchun 130033, China;

Corresponding author: ZHAO Li-Chun,
Received Date: 10 January 2012
Available Online: 21 March 2012
Fund Project: 本文是国家自然科学基金(No.21175055) (No.21175055) 吉林省科技厅项目(Nos.2011713,20110739)资助 (Nos.2011713,20110739)

The phosphorylation modification of talin, especially the phosphorylation state of talin in pathological environment such as carcinoma, is closely relevant to carcinogenesis and metastasis process. In this study, talin protein was isolated from human colorectal carcinoma tissues by salt fractionation and ion exchange chromatography, followed by further purification by electrophoresis. The purified talin was subject to tryptic digestion. Either immobilized Fe³⁺ affinity chromatography or TiO₂ affinity chromatography was used to absorb the phosphorylated peptides under acidic condition, which were then eluted with 1% ammonium hydroxide. The separation was performed on a Michrom Magic C₁₈ column with gradient elution using two mobile phase solutions (A:99% water+1% ACN+0.1% formic acid; B:99% ACN+1% water+0.1% formic acid). ESI mass spectrometer was used to detect the product ions of the eluted peaks under data-dependent acquisition mode. The results indicated that 8 phosphorylated peptides were captured by the immobilized Fe³⁺ affinity chromatography, whereas 9 phosphorylated peptides were captured by TiO₂ affinity chromatography. The present study provides a rapid, accurate method for characterizing talin protein isolated from human colorectal carcinoma tissues.
- Mass spectrometry,
- Talin,
- Colorectal carcinoma,
- Phosphorylation
1. [1]
  1 Cram E J, Schwarzbauer J E. Trends Cell Biol., 2004, 14(2): 55-57
2. [2]
  2 Zhang X, Jiang G, Cai Y, Monkley S J, Critchley D R, Sheetz M P. Nature Cell Biology, 2008, 10(9): 1062-1068
3. [3]
  3 Desiniotis A, Kyprianou N. Int. Rev. Cell Mol. Biol., 2011, 289: 117-147
4. [4]
  4 YANG Hong-Jun, DINF Yan-Qing, ZHOU Jun, YANG Lei. Chinese Journal of Clinical Oncology , 2008, 35(3): 139-141
5. [5]
  杨红军, 丁彦青, 周军, 杨磊. 中国肿瘤临床, 2008, 35(3): 139-141
6. [6]
  5 LIU Yong, GU Kang-Sheng. Chinese Clinical Oncology,2009,14(9): 787-791
7. [7]
  刘勇, 顾康生. 临床肿瘤学杂志,2009,14(9): 787-791
8. [8]
  6 Marcin P I, Rachel A D, Mei R N, Melissa M, Gaudenz D, Tan I, Joan S B. Cancer Discovery, 2011, 1(2): 144-157
9. [9]
  7 Olsen J V, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M. Cell, 2006, 127(3): 635-648
10. [10]
  8 Huang C, Rajfur Z, Yousefi N, Chen Z, Jacobson K, Ginsberg MH. Nature Cell Biology, 2009, 11(5): 624-630
11. [11]
  9 Liu N, Wang G, Lee KC, Guan Y, Chen H, Cai Z. FASEB J.,2009, 23(10): 3377-3782
12. [12]
  10 LIN Qing, CHEN Ping, JI Xue-Tao, KE Cai-Huan, HUANG He-Qing. Chinese J. Anal. Chem.,2010, 38(2): 207-213
13. [13]
  林青, 陈平, 季学涛, 柯才焕, 黄河清. 分析化学,2010, 38(2): 207-213
14. [14]
  11 Liu N, Song W, Wang P, Lee KC, Cai Z, Chen H. Proteomics, 2010, 10(9): 1875-1879
15. [15]
  12 JIANG Wei, YU Gang. Chinese J. Anal. Chem.,2012, 40(1): 32-37
16. [16]
  江玮, 喻钢. 分析化学,2012, 40(1): 32-37
17. [17]
  13 ZHANG Xiao-Ming, LIU Bao-Hong, ZHANG Li-Hua, ZOU Han-Fa, CAO Jing, GAO Ming-Xia, TANG Jia, LIU Yun, YANG Peng-Yuan, ZHANG Yu-Kui. Sci. China Chem., 2010, 53(4): 685-694
18. [18]
  14 Boersema PJ, Foong LY, Ding VM, Lemeer S, van Breukelen B, Philp R, Boekhorst J, Snel B, den Hertog J, Choo AB, Heck A J. Mol Cell Proteomics, 2010, 9(1): 84-99
19. [19]
  15 Han G, Ye M, Zhou H, Jiang X, Feng S, Jiang X, Tian R, Wan D, Zou H, Gu J. Proteomics,2008, 8(7): 1346-1361
20. [20]
  16 Feng S, Pan C, Jiang X, Xu S, Zhou H, Ye M, Zou H. Proteomics,2007, 7(3): 351-360
21. [21]
  17 SHAN Zhe, HAN Lu, YU Xi-Juan, TU Bo, ZHAO Dong-Yuan, YANG Peng-Yuan. Chinese J. Anal. Chem., 2008, 36(7): 885-889
22. [22]
  单喆, 韩路, 于锡娟, 屠波, 赵东元, 杨芃原. 分析化学, 2008, 36(7): 885-889
23. [23]
  18 Kweon H K, Hkansson K. Anal. Chem.,2006, 78(6): 1743-1749
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沈阳化工大学材料科学与工程学院沈阳 110142