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Citation: LI Yan, LIU Xiaoyan, ZHANG Shun, LIU Ting, LI Yongjun, LAN Yanyu, WANG Aimin, WANG Yonglin, HE Bin. Synthesis and Application of a Fluorescently Labeled Coumarin Peptide[J]. Chinese Journal of Applied Chemistry, ;2014, 31(12): 1413-1418. doi: 10.3724/SP.J.1095.2014.40090 shu

Synthesis and Application of a Fluorescently Labeled Coumarin Peptide

LI Yan ^a ,
LIU Xiaoyan ^b,d ,
ZHANG Shun ^b,d ,
LIU Ting ^c,d ,
LI Yongjun ^b,d ,
LAN Yanyu ^b,d ,
WANG Aimin ^b,d ,
WANG Yonglin ^c,d ,
HE Bin ^b,d,,

1.
a College of Basic Medical Science, Guiyang Medical University, Guiyang 550004, China;
2.
b Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medince(Ministry of Education), Guiyang Medical University, Guiyang 550004, China;
3.
c Guizhou Provincial Key Laboratory of Pharmaceutics, Guiyang Medical College, Guiyang Medical University, Guiyang 550004, China;
4.
d College of Pharmacy, Guiyang Medical University, Guiyang 550004, China

Corresponding author: HE Bin,
Received Date: 24 March 2014
Available Online: 16 June 2014
Fund Project: 国家自然科学基金资助项目(21302027) (21302027)贵州省中药现代化科技产业研究开发专项项目(黔科合重G字[2013]4001) (黔科合重G字[2013]4001)贵州省科技支撑计划社会发展攻关([2012]3079) ([2012]3079)贵阳医学院院基金博士启动基金([2014]第015号) ([2014]第015号)

Sirtuin is a type of nicotinamide adenine dinucleotide(NAD) dependent histone deacetylase,which has seven members.It is a potential therapeutic target for many diseases.However,the present fluorescent screening method is limited to SIRT1~SIRT3.Therefore,based on the discovery of SIRT5's novel enzymatic activity,we designed and synthesized the fluorescence labeled peptide for SIRT5,ISGASE(SuK)-AMC;and by the validations of LC-MS and fluorescence detection,the fluorescence labeled peptide can be used as a tool to perform the screening based on the enzymatic activity of SIRT5.
- histone deacetylase,
- Sirtuin,
- SIRT5,
- fluorescently labeled peptides
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