Home

Citation: YANG Shaoming, ZHA Wenling, SUN Qin, LI Hong, LI Ruiqin, SHANG Peiling. Label-free Elechochemical Aptasensor Based on Nickel Hexacyanoferrate for the Detection of Thrombin[J]. Chinese Journal of Applied Chemistry, ;2014, 31(6): 742-748. doi: 10.3724/SP.J.1095.2014.30416 shu

Label-free Elechochemical Aptasensor Based on Nickel Hexacyanoferrate for the Detection of Thrombin

1.
Department of Chemistry and Chemical Engineering, School of Sciences, East China Jiaotong University, Nanchang 330013, China

Corresponding author: YANG Shaoming,
Received Date: 16 August 2013
Available Online: 11 October 2013
Fund Project: 国家自然科学基金资助项目(21065004) (21065004)江西省教育厅科技项目(GJJ12304) (GJJ12304)

A label-free electrochemical aptasensor has been constructed for the electrochemical detection of thrombin.Two layers of nickel hexacyanoferrate redox probes sandwiched with multi-walled carbon nanotubes for the sensitivity enhancement were directly immobilized on the electrode surface by electrodeposition,and gold nanoparticles was used as the immobilization matrix for the aptamers.SEM was used to characterize the morphologies of multi-walled carbon nanotubes and nickel hexacyanoferrate.Electrochemical impedance spectroscopy was used to monitor the self-assembly process of aptamer biosensors.Electrochemical behaviors of aptamer biosensors were studied by cyclic voltammetry and differential pulse voltammetry.The biosensor shows a good linear range of 1.0 ng/L~1.0 mg/L(R=0.998) for the electrochemical detection of thrombin with a detection limit of 0.2 ng/L(S/N=3).
- nickel hexacyanoferrate,
- aptamer,
- thrombin,
- biosensor
1. [1]
  Qilong Fang , Yiqi Li , Jiangyihui Sheng , Quan Yuan , Jie Tan . Magical Pesticide Residue Detection Test Strips: Aptamer-based Lateral Flow Test Strips for Organophosphorus Pesticide Detection. University Chemistry, 2024, 39(5): 80-89. doi: 10.3866/PKU.DXHX202310004
2. [2]
  Cun WANG , Shaohan XU , Yuqian ZHANG , Yaoyao ZHANG , Tao GONG , Rong WEN , Yuhang LIAO , Yanrong REN . Terbium complex electrochemiluminescent emitters: Synthesis and application in the detection of epinephrine. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1351-1360. doi: 10.11862/CJIC.20240427
3. [3]
  Pengcheng Yan , Peng Wang , Jing Huang , Zhao Mo , Li Xu , Yun Chen , Yu Zhang , Zhichong Qi , Hui Xu , Henan Li . Engineering Multiple Optimization Strategy on Bismuth Oxyhalide Photoactive Materials for Efficient Photoelectrochemical Applications. Acta Physico-Chimica Sinica, 2025, 41(2): 100014-0. doi: 10.3866/PKU.WHXB202309047
4. [4]
  Songmei Ma , Ying Zhang , Gang Liu , Wenlong Xu . Comprehensive Experiment Teaching Exploration and Practice in Polymeric Materials Integrating Research-Driven Learning, Creativity-Enhanced Competency, and Science-Education Synergy: A Case Study of Machine Learning-Assisted Intelligent Handwriting Recognition System. University Chemistry, 2026, 41(1): 289-297. doi: 10.12461/PKU.DXHX202509083
5. [5]
  Ke Zhao , Zhen Liu , Luyao Liu , Changyuan Yu , Jingshun Pan , Xuguang Huang . Functionalized Reflective Structure Fiber-Optic Interferometric Sensor for Trace Detection of Lead Ions. Acta Physico-Chimica Sinica, 2024, 40(4): 2304029-0. doi: 10.3866/PKU.WHXB202304029
6. [6]
  Qiaoqiao BAI , Anqi ZHOU , Xiaowei LI , Tang LIU , Song LIU . Construction of pressure-temperature dual-functional flexible sensors and applications in biomedicine. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2259-2274. doi: 10.11862/CJIC.20240128
7. [7]
  Lin′an CAO , Dengyue MA , Gang XU . Research advances in electrically conductive metal-organic frameworks-based electrochemical sensors. Chinese Journal of Inorganic Chemistry, 2025, 41(10): 1953-1972. doi: 10.11862/CJIC.20250160
8. [8]
  Xingchao Zhao , Xiaoming Li , Ming Liu , Zijin Zhao , Kaixuan Yang , Pengtian Liu , Haolan Zhang , Jintai Li , Xiaoling Ma , Qi Yao , Yanming Sun , Fujun Zhang . Photomultiplication-Type All-Polymer Photodetectors and Their Applications in Photoplethysmography Sensor. Acta Physico-Chimica Sinica, 2025, 41(1): 100007-0. doi: 10.3866/PKU.WHXB202311021
9. [9]
  Jiarong Feng , Yejie Duan , Chu Chu , Dezhen Xie , Qiu'e Cao , Peng Liu . Preparation and Application of a Streptomycin Molecularly Imprinted Electrochemical Sensor: A Suggested Comprehensive Analytical Chemical Experiment. University Chemistry, 2024, 39(8): 295-305. doi: 10.3866/PKU.DXHX202401016
10. [10]
  Yang Meiqing , Lu Wang , Haozi Lu , Yaocheng Yang , Song Liu . Recent Advances of Functional Nanomaterials for Screen-Printed Photoelectrochemical Biosensors. Acta Physico-Chimica Sinica, 2025, 41(2): 100018-0. doi: 10.3866/PKU.WHXB202310046
11. [11]
  Zhongxin YU , Wei SONG , Yang LIU , Yuxue DING , Fanhao MENG , Shuju WANG , Lixin YOU . Fluorescence sensing on chlortetracycline of a Zn-coordination polymer based on mixed ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2415-2421. doi: 10.11862/CJIC.20240304
12. [12]
  Linjie ZHU , Xufeng LIU . Electrocatalytic hydrogen evolution performance of tetra-iron complexes with bridging diphosphine ligands. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 321-328. doi: 10.11862/CJIC.20240207
13. [13]
  Guilan He , Yaofeng Yuan . 手性二茂铁双膦配体Xyliphos的合成及应用. University Chemistry, 2025, 40(8): 130-137. doi: 10.12461/PKU.DXHX202409122
14. [14]
  Linjie ZHU , Xufeng LIU . Synthesis, characterization and electrocatalytic hydrogen evolution of two di-iron complexes containing a phosphine ligand with a pendant amine. Chinese Journal of Inorganic Chemistry, 2025, 41(5): 939-947. doi: 10.11862/CJIC.20240416
15. [15]
  Wang Wang , Yucheng Liu , Shengli Chen . Use of NiFe Layered Double Hydroxide as Electrocatalyst in Oxygen Evolution Reaction: Catalytic Mechanisms, Electrode Design, and Durability. Acta Physico-Chimica Sinica, 2024, 40(2): 2303059-0. doi: 10.3866/PKU.WHXB202303059
16. [16]
  Nan Wu , Hang Zhang , Lingling Wei , Quan Gu , Haoquan Zheng , Weiqiang Zhang , Rui Cao . Cyanide: Poison or Treasure?. University Chemistry, 2025, 40(7): 177-188. doi: 10.12461/PKU.DXHX202409072
17. [17]
  Quanliang Chen , Zhaohui Zhou . Research on the Active Site of Nitrogenase over Fifty Years. University Chemistry, 2024, 39(7): 287-293. doi: 10.3866/PKU.DXHX202310133
18. [18]
  Shuhong Xiang , Lv Yang , Yingsheng Xu , Guoxin Cao , Hongjian Zhou . Selective electrosorption of Cs(Ⅰ) from high-salinity radioactive wastewater using CNT-interspersed potassium zinc ferrocyanide electrodes. Acta Physico-Chimica Sinica, 2025, 41(9): 100097-0. doi: 10.1016/j.actphy.2025.100097
19. [19]
  Heng Zhang . Determination of All Rate Constants in the Enzyme Catalyzed Reactions Based on Michaelis-Menten Mechanism. University Chemistry, 2024, 39(4): 395-400. doi: 10.3866/PKU.DXHX202310047
20. [20]
  Yerong Chen , Bingbin Yang , Xinglei He , Yuqi Lin , Keyin Ye . Enzyme-Directed Evolution Enables Bioconversion of Organosilicon Compounds. University Chemistry, 2025, 40(10): 121-129. doi: 10.12461/PKU.DXHX202411054

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(639)
HTML views(69)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888

DownLoad: Full-Size Img PowerPoint

Return