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Citation: YIN Xiaomei, YU Haitao, WANG Xiaolong. Synthesis and Characterization of a New Artificial Free Triiodothyronine Antigen[J]. Chinese Journal of Applied Chemistry, ;2013, 30(11): 1281-1284. doi: 10.3724/SP.J.1095.2013.20570 shu

Synthesis and Characterization of a New Artificial Free Triiodothyronine Antigen

School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China

Received Date: 10 December 2012
Revised Date: 18 March 2013

FT₃C hapten was synthesized via a fast,mild "Click Chemistry",which contained a "spacer arm" of triazole ring conjugated to carboxyl site of free triiodothyronine(FT₃).Immunogen and coating antigen of FT₃C were respectively prepared by the coupling reaction between hapten and bovine serum albumin(BSA) or keyhole limpet hemocyanin(KLH),using the N-hydroxysuccinimide active ester method. UV spectrum of the artificial antigen(FT₃C-BSA) differed significantly from those of hapten(FT₃C) and BSA,the results also showed that FT₃C was conjugated to BSA successfully,the coupling ratio of hapten to BSA was 7:1;the antibody with high titer(6.24 × 10⁴) and IC₅₀value of 0.37 mg/L was produced through immunization of Balb/c mice.This study provided a new way to prepare FT₃ artificial antigen.
- free triiodothyronine,
- Click Chemistry,
- hapten,
- artificial antigen
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