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Citation: MA De-Zhang, DIAO Yu-Wen, YANG Jia-Xin, WANG Bao-De, YU Yuan-Hua. ELISA Detection of Steroidal Hormones in Water Resources,Food and Feed[J]. Chinese Journal of Applied Chemistry, ;2010, 27(12): 1466-1469. doi: 10.3724/SP.J.1095.2010.00067 shu

ELISA Detection of Steroidal Hormones in Water Resources,Food and Feed

a. School of Life Science and Technology, Changchun University of Science and Technology, Changchun 130022;
b. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130022;
c. Changchun Corporation of Chinese Oil Transportation Pipeline, Changchun 130022

Received Date: 26 January 2010
Revised Date: 10 May 2010

Comamonas testosteroni secretes 3α-HSD/CR when induced by steroid hormones,then it has the characteristics to degrade steroid hormones.This work developed a Enzyme-linked immunosorbent assay(ELISA) method to detect the steroidal hormones in water,food and animal feed.The results show that the content of testosterone and 3α-HSD enzyme expression have a linear relationship.The linear range is within 0.5~600 μg/L.Adopting this method,the study detect the steroidal hormones in water,food and feed.Content of testosterone in Songhua Lake is 34.5 μg/L,which is higher than others.Content of testosterone in the 8 animal feed is more than 2 μg/L.Meat sample test shows that the amount of steroidal hormones is below the detection limit.This result from this method is consistent with that from HPLC.
- steroid hormones,
- Enzyme-linked immunosorbent assay,
- 3α-HSD/CR
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