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Citation: LI Xia, XIONG Hai-Rong, JIANG Li-Hua, WEN Zhu-Youd, XIONG Yuan-Fu. Determination of Galanthamine and Lycorine in Lycoris Herb. by HPLC[J]. Chinese Journal of Applied Chemistry, ;2010, 27(11): 1362-1364. doi: 10.3724/SP.J.1095.2010.00002 shu

Determination of Galanthamine and Lycorine in Lycoris Herb. by HPLC

a. College of Science, Hu'nan Agricultural University, Changsha 410128;
b. Center of Analysis and Testing, Hu'nan Agricultural University, Changsha 410128;
c. Changsha Environmental Protection College, Hu'nan Agricultural University, Changsha 410128;
d. College of Animal Science and Technology, Hu'nan Agricultural University, Changsha 410128

Received Date: 4 January 2010
Revised Date: 19 June 2010

A high performance liquid chromatographic method was developed for simultaneous determining the contents of galanthamine and lycorine in Lycoris Herb. The chromatographic analysis was carried out on a phenomenex-C₁₈ column. The mobile phase was acetonitrile (A)-0.1% trifluoroacetic acid(TFA) (B) with gradient elution. The detection wavelength was set at 289 nm. The calibration curves for galanthamine and lycorine showed good linearity in the range of 0.5~10 mg/L(r=0.9999). The detection limits were 0.09 mg/L for galanthamine and 0.15 mg/L for lycorine at the signal-to-noise ratio of 3,and the average recoveries were 99.53% and 96.82%,respectively. The method is simple,rapid,accurate and suitable for the determination of galanthamine and lycorine in Lycoris Herb.
- HPLC,
- Lycoris Herb,
- galanthamine,
- lycorine
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