Citation: YANG Jing-Bo, HE Cheng-Yan, LU Ri-Feng, LIU Ning. Proteomic Analysis of Palmitoylated Proteins in Human Colon Carcinoma Tissues[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(10): 1465-1472. doi: 10.19756/j.issn.0253-3820.221414 shu

Proteomic Analysis of Palmitoylated Proteins in Human Colon Carcinoma Tissues

1.
Central Laboratory, the Second Hospital of Jilin University, Changchun 130041, China;
2.
Department of Clinical Laboratory, China-Japan Union Hospital of Jilin University, Changchun 130033, China;
3.
College of Public Health, Jilin University, Changchun 130012, China

Corresponding author: LIU Ning, liu_ning@jlu.edu.cn
Received Date: 12 August 2022
Revised Date: 24 August 2022

Fund Project: Supported by the Science and Technology Department of Jilin Province, China (Nos.20210204103YY, 20180101267JC).

Protein palmitoylation plays important roles in the occurrence, development and metastasis of colon cancer. In this study, acyl biotinyl exchange (ABE) method was improved and used to enrich palmitoylated proteins in colorectal cancer tissues. The enriched proteins were identified by proteomics approaches. A total of 213 highly reliable palmitoylated proteins(+HA/-HA>3) were identified, including enolase 1, peroxide oxidoreductase, vimentin, mitochondrial malate dehydrogenase, nuclear heterogeneous ribonucleoprotein K, and chaperone complex protein 1. The palmitoylation modification of enolase 1 was verified by Western blot analysis. The present study improved the classical ABE method to reduce its non-specific background. The detection results of palmitoylated proteins in colon cancer tissues could provide a new idea for further studying the molecular mechanism of palmitoylation of specific tumor related proteins and accurately discovering tumor markers or molecular targets of colon cancer.
- Palmitoylation,
- Colon carcinoma,
- Proteome,
- Acyl-biotin-exchange,
- Enolase 1
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沈阳化工大学材料科学与工程学院沈阳 110142