Citation: JIANG Chun-Feng, XUE Guang-Hui, ZHANG Jin-Qiu, FU Li-Juan, GAO Ying, GAO Lu. Study on Antidepressant Mechanism of Chaihuyujinxiang Granules Based on Ultra-High Performance Liquid Chromatography Coupled With Quadrupole Time-of-Flight Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2023, 51(4): 579-588. doi: 10.19756/j.issn.0253-3820.221413 shu

Study on Antidepressant Mechanism of Chaihuyujinxiang Granules Based on Ultra-High Performance Liquid Chromatography Coupled With Quadrupole Time-of-Flight Mass Spectrometry

JIANG Chun-Feng ¹ ,
XUE Guang-Hui ¹ ,
ZHANG Jin-Qiu ² ,
FU Li-Juan ² ,
GAO Ying ^1,, ,
GAO Lu ^1,2,,

1.
College of Pharmacy, Changchun University of Traditional Chinese Medicine, Changchun 130117, China;
2.
Jilin Modern Chinese Medicine Engineering Research Center Co., Ltd., Changhcun 130000, China

Corresponding author: GAO Ying, GAO Lu,
Received Date: 12 August 2022
Revised Date: 2 December 2022

Fund Project: Supported by the Jilin Provincial Science and Technology Development Program (No.20210204061YY).

The treatment mechanism of depression of model mice induced by chronic unpredictable mild stress (CUMS) using Chaihuyujinxiang granules (CHYJX) was investigated by ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS), which laid a theoretical foundation for the clinical treatment of depression using CHYJX. A total of 1266 endogenous metabolites were identified in mouse plasma. Based on univariate statistical analysis, there were significant differences in metabolites between the blank group and the model group, and between the model group and the CHYJX treatment group. The results of principal component analysis (PCA) of multidimensional statistical analysis showed that there was a clear trend of difference between the blank group, the model group and the CHYJX treatment group. The orthogonal partial least squares discriminant analysis method (OPLS-DA) could clearly distinguish the blank group and the model group, and the model group and the CHYJX treatment group, and 57 and 89 differential metabolites were screened out, respectively, of which 13 differential metabolites had opposite trends in the two contrast modes. The pathway enrichment analysis of these 13 differential metabolites showed that GABAergic synapses, neural active ligand receptor interactions, synaptic vesicle circulation, 5-hydroxytryptaminergic and dopaminergic synapses, sphingolipid metabolism, phenylalanine and tyrosine, tryptophan biosynthesis, and phenylalanine metabolic pathway had significant changes. The pathogenesis of depression was closely related to neural activity. The results of this study indicated that the mechanism of CHYJX in the treatment of CUMS and solitary-induced depression model mice was closely related to these eight signaling pathways.
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