直接随机光学重构显微镜研究葡萄糖对INS-1细胞膜上突触融合蛋白1A的调控机制

赵关芳 蔡明军 李泓儒 邹天一 高婧 徐海娇 王宏达

引用本文: 赵关芳, 蔡明军, 李泓儒, 邹天一, 高婧, 徐海娇, 王宏达. 直接随机光学重构显微镜研究葡萄糖对INS-1细胞膜上突触融合蛋白1A的调控机制[J]. 分析化学, 2023, 51(1): 63-71. doi: 10.19756/j.issn.0253-3820.221378 shu
Citation:  ZHAO Guan-Fang,  CAI Ming-Jun,  LI Hong-Ru,  ZOU Tian-Yi,  GAO Jing,  XU Hai-Jiao,  WANG Hong-Da. Mechanism Study of Glucose Regulation of Syntaxin 1A on INS-1 Cell Membranes by Direct Stochastic Optical Reconstruction Microscopy[J]. Chinese Journal of Analytical Chemistry, 2023, 51(1): 63-71. doi: 10.19756/j.issn.0253-3820.221378 shu

直接随机光学重构显微镜研究葡萄糖对INS-1细胞膜上突触融合蛋白1A的调控机制

    通讯作者: 徐海娇,E-mail:xuhj@ciac.ac.cn; 王宏达,E-mail:hdwang@ciac.ac.cn
  • 基金项目:

    国家自然科学基金项目(Nos.21727816,21721003,22150003)和中国科学院科学仪器研制项目(No.ZDKYYQ20220005)资助。

摘要: 胰岛素的分泌受葡萄糖浓度的影响。突触融合蛋白1A(Syntaxin 1A,STX-1A)是可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体(Soluble N-ethylmaleimide-sensitive factor attachment proteins receptor,SNARE)复合物的核心组件,对胰岛素分泌过程中胰岛素颗粒与细胞膜的锚定和融合至关重要。葡萄糖是否对STX-1A具有调控作用以及如何调控和影响胰岛素的释放的机制尚不清楚。本研究通过直接随机光学重构显微镜(Direct stochastic optical reconstruction microscopy,dSTORM)研究了葡萄糖浓度对INS-1细胞膜上的STX-1A的影响。研究发现,随着葡萄糖浓度升高,STX-1A在细胞膜上的表达量增加,STX-1A蛋白簇的密度和聚集程度也增加。但是,高浓度葡萄糖长时间刺激导致的糖毒性严重降低了细胞膜上STX-1A蛋白的表达量;同时,细胞膜上STX-1A蛋白簇的密度和聚集程度也显著降低。本研究将STX-1A在细胞膜上的表达和空间分布特征与胰岛素分泌细胞功能相关联,揭示了葡萄糖在分子水平上对STX-1A功能组织的调节作用,为研究SNAREs调节胰岛素释放的机制提供了新视角。

English


    1. [1]

      PRATLEY R E, WEYER C. Diabetologia, 2001, 44(8):929-945.PRATLEY R E, WEYER C. Diabetologia, 2001, 44(8):929-945.

    2. [2]

      KHAN A, PESSIN J. Diabetologia, 2002, 45(11):1475-1483.KHAN A, PESSIN J. Diabetologia, 2002, 45(11):1475-1483.

    3. [3]

      LUO X, WU J, JING S, YAN L. J. Aging Dis, 2016, 7(1):90-110.LUO X, WU J, JING S, YAN L. J. Aging Dis, 2016, 7(1):90-110.

    4. [4]

      OLSON L K, REDMON J B, TOWLE H C, ROBERTSON R P. J. Clin. Invest., 1993, 92(1):514-519.OLSON L K, REDMON J B, TOWLE H C, ROBERTSON R P. J. Clin. Invest., 1993, 92(1):514-519.

    5. [5]

      PARK K G, LEE K M, SEO H Y, SUH J H, KIM H S, WANG L, WON K C, LEE H W, PARK J Y, LEE K U, KIM J G, KIM B W, CHOI H S, LEE I K. Diabetes, 2007, 56(2):431-437.PARK K G, LEE K M, SEO H Y, SUH J H, KIM H S, WANG L, WON K C, LEE H W, PARK J Y, LEE K U, KIM J G, KIM B W, CHOI H S, LEE I K. Diabetes, 2007, 56(2):431-437.

    6. [6]

      YANG G Y, LI L J, LIU Y M, LIANG K, WEI L S, CHEN L Y. Front. Cell Dev. Biol., 2021, 9:650167.YANG G Y, LI L J, LIU Y M, LIANG K, WEI L S, CHEN L Y. Front. Cell Dev. Biol., 2021, 9:650167.

    7. [7]

      LANG J. EJBio, 1999, 259(1-2):3-17.LANG J. EJBio, 1999, 259(1-2):3-17.

    8. [8]

      GAUTHIER B R, WOLLHEIM C B. Am. J. Physiol.:Endocrinol. Metab., 2008, 295(6):E1279-E1286.GAUTHIER B R, WOLLHEIM C B. Am. J. Physiol.:Endocrinol. Metab., 2008, 295(6):E1279-E1286.

    9. [9]

      GAISANO H Y. Diabetes, Obes. Metab., 2017, 19(S1):115-123.GAISANO H Y. Diabetes, Obes. Metab., 2017, 19(S1):115-123.

    10. [10]

      NOMIYAMA R, EMOTO M, FUKUDA N, MATSUI K, KONDO M, SAKANE A, SASAKI T, TANIZAWA Y. J. Diabetes Invest., 2019, 10(3):591-601.NOMIYAMA R, EMOTO M, FUKUDA N, MATSUI K, KONDO M, SAKANE A, SASAKI T, TANIZAWA Y. J. Diabetes Invest., 2019, 10(3):591-601.

    11. [11]

      AMOS C, KIESSLING V, SCHENK N, MOHAN R, DOYLE C A., KREUTZBERGER A J B, WANG H Y, LEVENTAL K R, LEVENTAL I, ANANTHARAM A, TAMM L K. Biomed. Pharmacol. J., 2022, 121(3):292a-293a.AMOS C, KIESSLING V, SCHENK N, MOHAN R, DOYLE C A., KREUTZBERGER A J B, WANG H Y, LEVENTAL K R, LEVENTAL I, ANANTHARAM A, TAMM L K. Biomed. Pharmacol. J., 2022, 121(3):292a-293a.

    12. [12]

      LAM P P L, LEUNG Y M, SHEU L, ELLIS J, TSUSHIMA R G, OSBORNE L R, GAISANO H Y. Diabetes, 2005, 54(9):2744-2754.LAM P P L, LEUNG Y M, SHEU L, ELLIS J, TSUSHIMA R G, OSBORNE L R, GAISANO H Y. Diabetes, 2005, 54(9):2744-2754.

    13. [13]

      KOFUJI T, FUJIWARA T, SANADA M, MISHIMA T, AKAGAWA K. J. Neurosci., 2014, 130(4):514-525.KOFUJI T, FUJIWARA T, SANADA M, MISHIMA T, AKAGAWA K. J. Neurosci., 2014, 130(4):514-525.

    14. [14]

      FUKUDA R, MCNEW J A, WEBER T, PARLATI F, ENGEL T, NICKEL W, ROTHMAN J E, SÖLLNER T H. Nature, 2000, 407(6801):198-202.FUKUDA R, MCNEW J A, WEBER T, PARLATI F, ENGEL T, NICKEL W, ROTHMAN J E, SÖLLNER T H. Nature, 2000, 407(6801):198-202.

    15. [15]

      HONG W J, LEV S. Trends Cell Biol., 2014, 24(1):35-43.HONG W J, LEV S. Trends Cell Biol., 2014, 24(1):35-43.

    16. [16]

      PFEFFER S R. Annu. Rev. Cell Dev. Biol., 1996, 12(1):441-461.PFEFFER S R. Annu. Rev. Cell Dev. Biol., 1996, 12(1):441-461.

    17. [17]

      XIONG Q Y, YU C, ZHANG Y, LING L F, WANG L Z, GAO J L. Biomed. Rep., 2017, 6(2):134-139.XIONG Q Y, YU C, ZHANG Y, LING L F, WANG L Z, GAO J L. Biomed. Rep., 2017, 6(2):134-139.

    18. [18]

      JAHN R, SCHELLER R H. Nat. Rev. Mol. Cell Biol., 2006, 7(9):631-643.JAHN R, SCHELLER R H. Nat. Rev. Mol. Cell Biol., 2006, 7(9):631-643.

    19. [19]

      LIU W, PARPURA V. Sci. World J., 2010, 10:1258-1268.LIU W, PARPURA V. Sci. World J., 2010, 10:1258-1268.

    20. [20]

      YOON T Y, MUNSON M. Curr. Biol., 2018, 28(8):R397-R401.YOON T Y, MUNSON M. Curr. Biol., 2018, 28(8):R397-R401.

    21. [21]

      SOLIMENA M, SPEIER S. Cell Metab., 2010, 12(1):5-6.SOLIMENA M, SPEIER S. Cell Metab., 2010, 12(1):5-6.

    22. [22]

      NAGAMATSU S, NAKAMICHI Y, YAMAMURA C, MATSUSHIMA S, WATANABE T, OZAWA S, FURUKAWA H, ISHIDA H. Diabetes, 1999, 48(12):2367-2373.NAGAMATSU S, NAKAMICHI Y, YAMAMURA C, MATSUSHIMA S, WATANABE T, OZAWA S, FURUKAWA H, ISHIDA H. Diabetes, 1999, 48(12):2367-2373.

    23. [23]

      OSTENSON C G, GAISANO H, SHEU L, TIBELL A, BARTFAI T. Diabetes, 2006, 55(2):435-440.OSTENSON C G, GAISANO H, SHEU L, TIBELL A, BARTFAI T. Diabetes, 2006, 55(2):435-440.

    24. [24]

      TORREJÓN-ESCRIBANO B, ESCORIZA J, MONTANYA E, BLASI J. Endocrinology, 2011, 152(4):1290-1299TORREJÓN-ESCRIBANO B, ESCORIZA J, MONTANYA E, BLASI J. Endocrinology, 2011, 152(4):1290-1299

    25. [25]

      KLEIN T, PROPPERT S, SAUER M. Histochem. Cell Biol., 2014, 141(6):561-575KLEIN T, PROPPERT S, SAUER M. Histochem. Cell Biol., 2014, 141(6):561-575

    26. [26]

      LELEK M, GYPARAKI M T, BELIU G, SCHUEDER F, GRIFFIÉ J, MANLEY S, JUNGMANN R, SAUER M, LAKADAMYALI M, ZIMMER C. Nat. Rev. Methods Primers, 2021, 1(1):39.LELEK M, GYPARAKI M T, BELIU G, SCHUEDER F, GRIFFIÉ J, MANLEY S, JUNGMANN R, SAUER M, LAKADAMYALI M, ZIMMER C. Nat. Rev. Methods Primers, 2021, 1(1):39.

    27. [27]

      JENSEN E, CROSSMAN D J. Anat. Rec., 2014, 297(12):2227-2231.JENSEN E, CROSSMAN D J. Anat. Rec., 2014, 297(12):2227-2231.

    28. [28]

      YAN Q Y, LU Y T, ZHOU L L, CHEN J L, XU H J, CAI M J, SHI Y, JIANG J G, XIONG W Y, GAO J, WANG H D. Proc. Natl. Acad. Sci. U. S. A., 2018, 115(27):7033-7038.YAN Q Y, LU Y T, ZHOU L L, CHEN J L, XU H J, CAI M J, SHI Y, JIANG J G, XIONG W Y, GAO J, WANG H D. Proc. Natl. Acad. Sci. U. S. A., 2018, 115(27):7033-7038.

    29. [29]

      FU Y L, JING Y Y, GAO J, LI Z H, WANG H D, CAI M J, TONG T. Talanta, 2020, 207:120312.FU Y L, JING Y Y, GAO J, LI Z H, WANG H D, CAI M J, TONG T. Talanta, 2020, 207:120312.

    30. [30]

      OVESNY M, KŘÍŽEK P, BORKOVEC J, ŠVINDRYCH Z, HAGEN G M. Bioinformatics, 2014, 30(16):2389-2390OVESNY M, KŘÍŽEK P, BORKOVEC J, ŠVINDRYCH Z, HAGEN G M. Bioinformatics, 2014, 30(16):2389-2390

    31. [31]

      OWEN D M, RENTERO C, ROSSY J, MAGENAU A, WILLIAMSON D, RODRIGUEZ M, GAUS K. J. Biophotonics, 2010, 3(7):446-454.OWEN D M, RENTERO C, ROSSY J, MAGENAU A, WILLIAMSON D, RODRIGUEZ M, GAUS K. J. Biophotonics, 2010, 3(7):446-454.

    32. [32]

      LEVET F, HOSY E, KECHKAR A, BUTLER C, BEGHIN A, CHOQUET D, SIBARITA J B. Nat. Methods, 2015, 12(11):1065-1071.LEVET F, HOSY E, KECHKAR A, BUTLER C, BEGHIN A, CHOQUET D, SIBARITA J B. Nat. Methods, 2015, 12(11):1065-1071.

    33. [33]

      VAN DE LINDE S. J. Phys. D:Appl. Phys., 2019, 52(20):203002.VAN DE LINDE S. J. Phys. D:Appl. Phys., 2019, 52(20):203002.

    34. [34]

      KOMATSU M, TAKEI M, ISHII H, SATO Y. J. Diabetes Invest., 2013, 4(6):511-516.KOMATSU M, TAKEI M, ISHII H, SATO Y. J. Diabetes Invest., 2013, 4(6):511-516.

    35. [35]

      DING Jing-Yun, YING Ying, WU Lei, WU Yin, KONG Xiang-Chen, ZENG Qing, LIANG Zhen. Prog. Mod. Biomed., 2015, 15(22):4244-4247. 丁静云, 应颖, 邬磊, 伍茵, 孔祥臣, 曾庆, 梁真. 现代医学生物进展, 2015, 15(22):4244-4247.

    36. [36]

      KAISER N, LEIBOWITZ G, NESHER R. J. Pediatr. Endocrinol. Metab., 2003, 16(1):5-22.KAISER N, LEIBOWITZ G, NESHER R. J. Pediatr. Endocrinol. Metab., 2003, 16(1):5-22.

    37. [37]

      BENSELLAM M, LAYBUTT D R, JONAS J C. Mol. Cell. Endocrinol., 2012, 364(1):1-27.BENSELLAM M, LAYBUTT D R, JONAS J C. Mol. Cell. Endocrinol., 2012, 364(1):1-27.

    38. [38]

      DUBOIS M, VACHER P, ROGER B, HUYGHE D, VANDEWALLE B, KERR-CONTE J, PATTOU F, MOUSTAIÏD-MOUSSA N, LANG J. Endocrinology, 2007, 148(4):1605-1614.DUBOIS M, VACHER P, ROGER B, HUYGHE D, VANDEWALLE B, KERR-CONTE J, PATTOU F, MOUSTAIÏD-MOUSSA N, LANG J. Endocrinology, 2007, 148(4):1605-1614.

  • 加载中
计量
  • PDF下载量:  0
  • 文章访问数:  56
  • HTML全文浏览量:  1
文章相关
  • 收稿日期:  2022-07-26
  • 修回日期:  2022-08-17
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

/

返回文章