填充型纳流液相色谱柱技术的研究进展

引用本文: 刘娅, 陈嘉伟, 占发旺, 张博. 填充型纳流液相色谱柱技术的研究进展[J]. 分析化学, 2023, 51(1): 1-10. doi: 10.19756/j.issn.0253-3820.221345 shu

Citation: LIU Ya, CHEN Jia-Wei, ZHAN Fa-Wang, ZHANG Bo. Advance in Packed Column Technologies for Nanoflow Liquid Chromatography[J]. Chinese Journal of Analytical Chemistry, 2023, 51(1): 1-10. doi: 10.19756/j.issn.0253-3820.221345 shu

填充型纳流液相色谱柱技术的研究进展

刘娅 ^1, ,
陈嘉伟 ^1, ,
占发旺 ^{2,
,} ,
张博 ^{1,
,}

1.
厦门大学化学化工学院, 厦门 361005;
2.
江西万拓环境检测有限公司, 上饶 334000

通讯作者: 占发旺,E-mail:zhanfawanglzw@163.com; 张博,E-mail:bozhang@xmu.edu.cn

基金项目:
国家自然科学基金项目(Nos.21475110,J1310024)和厦门市海洋与渔业发展专项基金项目(No.19CZB001HJ03)资助。

摘要: 纳流液相色谱是高效液相色谱微型化的重要形式,由于其具有溶剂消耗少、样品需求量小、检测灵敏度高以及与质谱联用兼容性好等特点,在生命科学、生物医药以及微纳分析等领域得到越来越广泛的应用。填充柱是最常用的纳流色谱柱类型,通常由球形色谱填料颗粒填充入毛细管内制成。样品组分是在色谱柱上实现分离,色谱柱的性能对色谱分析的选择性和分辨率至关重要,因此制备高质量的纳流色谱填充柱对获得高效的纳流液相色谱分离尤为关键。纳流色谱柱的制备包含两个重要技术环节:柱塞技术和填充工艺。近年来,随着柱塞与填充技术的进步,填充型纳流液相色谱柱的稳定性和分离性能得到了显著提升。本文对近十年来纳流液相色谱柱技术的研究进展,主要是柱塞技术和填充技术的研究和应用进展进行了概述,介绍了其在组学研究和生物制药领域中的应用,并对其发展前景进行了展望。

关键词:

English

Advance in Packed Column Technologies for Nanoflow Liquid Chromatography

LIU Ya ^1, ,
CHEN Jia-Wei ^1, ,
ZHAN Fa-Wang ^{2,
,} ,
ZHANG Bo ^{1,
,}

1.
College of Chemistry&Chemical Engineering, Xiamen University, Xiamen 361005, China;
2.
Jiangxi Wantuo Environmental Testing Co. Ltd, Shangrao 334000, China

Corresponding author: ZHAN Fa-Wang, ; ZHANG Bo,

Received Date: 12 July 2022
Revised Date: 28 August 2022
Fund Project:
Supported by the National Natural Science Foundation of China (Nos.21475110, J1310024) and the Xiamen Marine and Fishery Development Foundation Project (No.19CZB001HJ03).

Abstract: As an important format of the miniaturization of high performance liquid chromatography, nanoflow liquid chromatography has been widely used in the fields of life science, biomedicine and microscale analysis, etc, due to its unique advantages such as less consumption of solvent, less demand of sample amount, higher sensitivity and better compatibility with mass spectrometer. As the core of nanoflow liquid chromatography, packed columns are the most commonly used column type, which are prepared by packing spherical chromatographic materials in the capillary tube. Since column is the place where the separation actually takes place, it plays an important role in the resolution and selectivity of chromatographic separations. Therefore, preparation of high resolution microcolumns is of great importance in securing high performance nano-LC separations. There are two key technologies in preparation of nano-LC columns: fritting and packing. In recent years, with the continuous development of fritting and packing technologies, the stability and separation performance of packed nanoflow liquid chromatographic columns have been significantly improved. This review summarized the developments in fritting and packing technologies involved in nanoflow liquid chromatographic columns in the past ten years, and discussed the recent applications in the field of omics research and biopharmaceuticals, as well as its prospects for future developments.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

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通讯作者: 占发旺,E-mail:zhanfawanglzw@163.com; 张博,E-mail:bozhang@xmu.edu.cn

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Advance in Packed Column Technologies for Nanoflow Liquid Chromatography

Corresponding author: ZHAN Fa-Wang, ; ZHANG Bo,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

填充型纳流液相色谱柱技术的研究进展

通讯作者: 占发旺,E-mail:zhanfawanglzw@163.com; 张博,E-mail:bozhang@xmu.edu.cn

English

Advance in Packed Column Technologies for Nanoflow Liquid Chromatography

Corresponding author: ZHAN Fa-Wang, ; ZHANG Bo,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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