填充型纳流液相色谱柱技术的研究进展

刘娅 陈嘉伟 占发旺 张博

引用本文: 刘娅, 陈嘉伟, 占发旺, 张博. 填充型纳流液相色谱柱技术的研究进展[J]. 分析化学, 2023, 51(1): 1-10. doi: 10.19756/j.issn.0253-3820.221345 shu
Citation:  LIU Ya,  CHEN Jia-Wei,  ZHAN Fa-Wang,  ZHANG Bo. Advance in Packed Column Technologies for Nanoflow Liquid Chromatography[J]. Chinese Journal of Analytical Chemistry, 2023, 51(1): 1-10. doi: 10.19756/j.issn.0253-3820.221345 shu

填充型纳流液相色谱柱技术的研究进展

    通讯作者: 占发旺,E-mail:zhanfawanglzw@163.com; 张博,E-mail:bozhang@xmu.edu.cn
  • 基金项目:

    国家自然科学基金项目(Nos.21475110,J1310024)和厦门市海洋与渔业发展专项基金项目(No.19CZB001HJ03)资助。

摘要: 纳流液相色谱是高效液相色谱微型化的重要形式,由于其具有溶剂消耗少、样品需求量小、检测灵敏度高以及与质谱联用兼容性好等特点,在生命科学、生物医药以及微纳分析等领域得到越来越广泛的应用。填充柱是最常用的纳流色谱柱类型,通常由球形色谱填料颗粒填充入毛细管内制成。样品组分是在色谱柱上实现分离,色谱柱的性能对色谱分析的选择性和分辨率至关重要,因此制备高质量的纳流色谱填充柱对获得高效的纳流液相色谱分离尤为关键。纳流色谱柱的制备包含两个重要技术环节:柱塞技术和填充工艺。近年来,随着柱塞与填充技术的进步,填充型纳流液相色谱柱的稳定性和分离性能得到了显著提升。本文对近十年来纳流液相色谱柱技术的研究进展,主要是柱塞技术和填充技术的研究和应用进展进行了概述,介绍了其在组学研究和生物制药领域中的应用,并对其发展前景进行了展望。

English


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  • 收稿日期:  2022-07-12
  • 修回日期:  2022-08-28
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