Citation: LIU Ya, CHEN Jia-Wei, ZHAN Fa-Wang, ZHANG Bo. Advance in Packed Column Technologies for Nanoflow Liquid Chromatography[J]. Chinese Journal of Analytical Chemistry, ;2023, 51(1): 1-10. doi: 10.19756/j.issn.0253-3820.221345 shu

Advance in Packed Column Technologies for Nanoflow Liquid Chromatography

LIU Ya ¹ ,
CHEN Jia-Wei ¹ ,
ZHAN Fa-Wang ^2,, ,
ZHANG Bo ^1,,

1.
College of Chemistry&Chemical Engineering, Xiamen University, Xiamen 361005, China;
2.
Jiangxi Wantuo Environmental Testing Co. Ltd, Shangrao 334000, China

Corresponding author: ZHAN Fa-Wang, ZHANG Bo,
Received Date: 12 July 2022
Revised Date: 28 August 2022

Fund Project: Supported by the National Natural Science Foundation of China (Nos.21475110, J1310024) and the Xiamen Marine and Fishery Development Foundation Project (No.19CZB001HJ03).

As an important format of the miniaturization of high performance liquid chromatography, nanoflow liquid chromatography has been widely used in the fields of life science, biomedicine and microscale analysis, etc, due to its unique advantages such as less consumption of solvent, less demand of sample amount, higher sensitivity and better compatibility with mass spectrometer. As the core of nanoflow liquid chromatography, packed columns are the most commonly used column type, which are prepared by packing spherical chromatographic materials in the capillary tube. Since column is the place where the separation actually takes place, it plays an important role in the resolution and selectivity of chromatographic separations. Therefore, preparation of high resolution microcolumns is of great importance in securing high performance nano-LC separations. There are two key technologies in preparation of nano-LC columns: fritting and packing. In recent years, with the continuous development of fritting and packing technologies, the stability and separation performance of packed nanoflow liquid chromatographic columns have been significantly improved. This review summarized the developments in fritting and packing technologies involved in nanoflow liquid chromatographic columns in the past ten years, and discussed the recent applications in the field of omics research and biopharmaceuticals, as well as its prospects for future developments.
- Nanoflow liquid chromatography,
- Packed columns,
- Capillary columns,
- Omics,
- Review
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