不同生长阶段的长柄扁桃叶蛋白质组学分析及有机酸代谢通路研究

引用本文: 李婷婷, 贺玥玥, 张晶晶, 秦芳玲, 李聪, 陈邦, 申烨华. 不同生长阶段的长柄扁桃叶蛋白质组学分析及有机酸代谢通路研究[J]. 分析化学, 2023, 51(1): 72-83. doi: 10.19756/j.issn.0253-3820.221323 shu

Citation: LI Ting-Ting, HE Yue-Yue, ZHANG Jing-Jing, QIN Fang-Ling, LI Cong, CHEN Bang, SHEN Ye-Hua. Proteomics and Organic Acid Metabolic Pathways in Amygdalus Pedunculata Pall Leaves at Different Growth Periods[J]. Chinese Journal of Analytical Chemistry, 2023, 51(1): 72-83. doi: 10.19756/j.issn.0253-3820.221323 shu

不同生长阶段的长柄扁桃叶蛋白质组学分析及有机酸代谢通路研究

李婷婷 ^1, ,
贺玥玥 ^1, ,
张晶晶 ^2, ,
秦芳玲 ^3, ,
李聪 ^1, ,
陈邦 ^1, ,
申烨华 ^{1,
,}

1.
西北大学化学与材料科学学院, 合成与天然功能分子教育部重点实验室, 西安 710127;
2.
西北大学化工学院, 西安 710069;
3.
西安石油大学化学化工学院, 西安 710065

通讯作者: 申烨华,E-mail:yhshen@nwu.edu.cn

基金项目:
国家自然科学基金项目(No.21675125)和陕西省科技计划项目(Nos.2018ZDXM-NY-087,2019TSLNY03-02)资助。

摘要: 以长柄扁桃叶片为研究对象,基于蛋白质组学分析,探索了不同生长阶段的长柄扁桃叶片中蛋白质的种类、含量和代谢途径的变化规律。研究结果显示,通过TMT标记定量蛋白质组学检测出6584种可信蛋白,筛选得到1678种差异蛋白。GO功能富集结果表明,差异蛋白参与了防御反应、对生物刺激的反应等生物过程,主要具有信号受体活性、蛋白磷酸酶抑制剂活性、谷胱甘肽转移酶活性和谷胱甘肽脱氢酶(抗坏血酸)活性等分子功能。从开花期到果实成熟期,长柄扁桃叶片中有机酸类化合物的含量逐渐下降,通过KEGG分析发现其主要与α-亚麻酸代谢、乙醛酸和二羧酸代谢以及光合生物中的碳固定这3条代谢通路有关,创伤酸、酮戊二酸、苹果酸和柠檬酸及12种相关蛋白对不同生长期长柄扁桃叶中有机酸类化合物的代谢过程具有重要作用。

关键词:

English

Proteomics and Organic Acid Metabolic Pathways in Amygdalus Pedunculata Pall Leaves at Different Growth Periods

1.
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710127, China;
2.
School of Chemical Engineering, Northwest University, Xi'an 710069, China;
3.
College of Chemistry and Chemical Engineering, Xi'an Shiyou University, Xi'an 710065, China

Corresponding author: SHEN Ye-Hua, yhshen@nwu.edu.cn

Received Date: 30 June 2022
Revised Date: 25 September 2022
Fund Project:
Supported by the National Natural Science Foundation of China (No.21675125) and the Shaanxi Provincial Science and Technology Program (Nos.2018ZDXM-NY-087, 2019TSLNY03-02).

Abstract: Based on proteomic analysis, the changes of protein species, content and metabolic pathway in the leaves of Amygdalus pedunculata Pall at different growth periods were investigated and clarified. The results showed that 6584 credible proteins were detected by tandem mass tags (TMT) labeled quantitative proteomics, and 1678 differential proteins were screened. The results of GO functional enrichment showed that the differential proteins participated in the biological processes such as defense response and response to biological stimulation, and mainly had molecular functions such as signal receptor activity, protein phosphatase inhibitor activity, glutathione transferase activity and glutathione dehydrogenase (ascorbic acid) activity.The content of organic acids in Amygdalus pedunculata Pall leaves decreased gradually from flowering stage to fruit ripening stage. Lyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that it was mainly related to α-linolenic acid metabolism, glyoxylic acid and dicarboxylic acid metabolism and carbon fixation in photosynthetic organisms. Traumatic acid, ketoglutarate, malic acid, citric acid and 12 related proteins played important roles in the metabolic process of organic acids in the leaves of Amygdalus pedunculata Pall at different growth stages.

Key words:

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

不同生长阶段的长柄扁桃叶蛋白质组学分析及有机酸代谢通路研究

通讯作者: 申烨华,E-mail:yhshen@nwu.edu.cn

English

Proteomics and Organic Acid Metabolic Pathways in Amygdalus Pedunculata Pall Leaves at Different Growth Periods

Corresponding author: SHEN Ye-Hua, yhshen@nwu.edu.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

不同生长阶段的长柄扁桃叶蛋白质组学分析及有机酸代谢通路研究

通讯作者: 申烨华,E-mail:yhshen@nwu.edu.cn

English

Proteomics and Organic Acid Metabolic Pathways in Amygdalus Pedunculata Pall Leaves at Different Growth Periods

Corresponding author: SHEN Ye-Hua, yhshen@nwu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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