基于上转换纳米粒子-金纳米棒的荧光共振能量转移免疫分析法用于癌胚抗原检测

引用本文: 于绍楠, 任玲玲, 任立群, 白应洁, 刘桂锋, 张婳, 王振新. 基于上转换纳米粒子-金纳米棒的荧光共振能量转移免疫分析法用于癌胚抗原检测[J]. 分析化学, 2022, 50(9): 1299-1307. doi: 10.19756/j.issn.0253-3820.221249 shu

Citation: YU Shao-Nan, REN Ling-Ling, REN Li-Qun, BAI Ying-Jie, LIU Gui-Feng, ZHANG Hua, WANG Zhen-Xin. Upconversion Nanoparticles/Gold Nanorods-based Fluorescence Resonance Energy Transfer Immunoassay for Detection of Carcinoembryonic Antigen[J]. Chinese Journal of Analytical Chemistry, 2022, 50(9): 1299-1307. doi: 10.19756/j.issn.0253-3820.221249 shu

基于上转换纳米粒子-金纳米棒的荧光共振能量转移免疫分析法用于癌胚抗原检测

于绍楠 ^1,2, ,
任玲玲 ^1, ,
任立群 ^2, ,
白应洁 ^1, ,
刘桂锋 ^{1,
,} ,
张婳 ^{3,
,} ,
王振新 ^3,

1.
吉林大学中日联谊医院放射线科, 长春 130033;
2.
吉林大学药学院, 长春 130021;
3.
中国科学院长春应用化学研究所, 电分析化学国家重点实验室, 长春 130022

通讯作者: 刘桂锋,E-mail:gfliu@jlu.edu.cn; 张婳,E-mail:zhanghua@ciac.ac.cn

基金项目:
吴阶平医学基金项目（Nos.320675019089-40，320675019089-38）和国家自然科学基金项目（No.61901438）资助。

摘要: 构建了一种基于上转换纳米粒子(Upconversion nanoparticles,UCNP)-金纳米棒(Gold nanorods,GNR)的荧光共振能量转移(Fluorescence resonance energy transfer,FRET)免疫分析方法用于灵敏检测癌胚抗原(Carcinoembryonic antigen,CEA)。以UCNP作为供体,GNR作为受体,将CEA抗体通过共价偶联的方式分别修饰在UCNP和GNR表面,形成抗体偶联物UCNP-cAb和GNR-dAb,当引入CEA后,通过抗原-抗体相互作用形成"三明治"夹心复合物,导致UCNP与GNR相互靠近发生FRET,并且荧光淬灭效率与CEA浓度呈正相关。基于此建立了检测CEA的FRET免疫分析方法,线性范围为0.01~100 ng/mL,检出限(S/N=3)为0.01 ng/mL。采用构建的FRET免疫分析方法实现了对缓冲溶液和人血清样本中的CEA的检测。本方法具有良好的选择性,有望用于临床检测,为相关癌症的早期诊断、治疗及预后监测等提供了新方法。

关键词:

English

Upconversion Nanoparticles/Gold Nanorods-based Fluorescence Resonance Energy Transfer Immunoassay for Detection of Carcinoembryonic Antigen

1.
Department of Radiology, China-Japan Union Hospital of Jilin University, Changchun 130033, China;
2.
School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China;
3.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Corresponding author: LIU Gui-Feng, ; ZHANG Hua,

Received Date: 19 May 2022
Revised Date: 15 July 2022
Fund Project:
Supported by the Wu Jieping Medical Foundation (Nos.320675019089-40, 320675019089-38) and the National Natural Science Foundation of China (No.61901438).

Abstract: A fluorescence resonance energy transfer (FRET) immunoassay based on upconversion nanoparticles (UCNP) and gold nanorods (GNR) was constructed for sensitive detection of carcinoembryonic antigen (CEA). UCNP was employed as the donor and GNR was employed as the acceptor. The CEA antibody was covalently attached on the surface of UCNP and GNR to form UCNP-cAb and GNR-dAb conjugates, respectively. After introduction of CEA, a "sandwich" complex was formed through the antigen-antibody specific interaction, which resulted in the close proximity of UCNP and GNR to generate FRET. And the fluorescence quenching efficiency was positively correlated with the concentration of CEA. The constructed FRET immunoassay realized the detection of CEA in both buffer solution and human serum samples with a limit of detection (S/N=3) of 0.01 ng/mL, a linear range from 0.01 ng/mL to 100 ng/mL and good selectivity. It was expected that this FRET immunoassay could be further used in clinical applications and provided new methods for early diagnosis, treatment and prognosis monitoring of related cancers.

Key words:

Upconversion nanoparticles
/ Gold nanorods
/ Fluorescence resonance energy transfer
/ Carcinoembryonic antigen
/ Quantitative detection

1. [1]
  CHEN W, ZHENG R, BAADE P D, ZHANG S, ZENG H. CA-Cancer J. Clin., 2016, 66:115-132.CHEN W, ZHENG R, BAADE P D, ZHANG S, ZENG H. CA-Cancer J. Clin., 2016, 66:115-132.
2. [2]
  ALTINTAS Z, TOTHILL I. Sens. Actuators, B, 2013, 188:988-998.ALTINTAS Z, TOTHILL I. Sens. Actuators, B, 2013, 188:988-998.
3. [3]
  SHEN G Y, WANG H, DENG T, SHEN G L, YU R Q. Talanta, 2005, 67:217-220.SHEN G Y, WANG H, DENG T, SHEN G L, YU R Q. Talanta, 2005, 67:217-220.
4. [4]
  GRUNNET M, SORENSEN J B. Lung Cancer, 2012, 76:138-143.GRUNNET M, SORENSEN J B. Lung Cancer, 2012, 76:138-143.
5. [5]
  FERRARI M. Nat. Rev. Cancer, 2005, 5:161-171.FERRARI M. Nat. Rev. Cancer, 2005, 5:161-171.
6. [6]
  DUFFY M J. Clin. Chem., 2001, 4:624-630.DUFFY M J. Clin. Chem., 2001, 4:624-630.
7. [7]
  HAIDOPOULOS D, KONSTADOULAKIS M M, ANTONAKIS P T, ALEXIOU D G, MANOURAS A M, KATSARAGAKIS S M, ANDROULAKIS G F. Euro. J. Surg. Oncol., 2000, 26:742-746.HAIDOPOULOS D, KONSTADOULAKIS M M, ANTONAKIS P T, ALEXIOU D G, MANOURAS A M, KATSARAGAKIS S M, ANDROULAKIS G F. Euro. J. Surg. Oncol., 2000, 26:742-746.
8. [8]
  ZHAO L, XU S, FJAERTOFT G, PAUKSEN K, HAKANSSON L, VENGE P. J. Immunol. Methods, 2004, 293:207-214.ZHAO L, XU S, FJAERTOFT G, PAUKSEN K, HAKANSSON L, VENGE P. J. Immunol. Methods, 2004, 293:207-214.
9. [9]
  THOMSON D M, KRUPEY J, FREEDMAN S O, GOLD P. Proc. Natl. Acad. Sci. U.S.A., 1969, 64:161-167.THOMSON D M, KRUPEY J, FREEDMAN S O, GOLD P. Proc. Natl. Acad. Sci. U.S.A., 1969, 64:161-167.
10. [10]
  NIKOOYEH B, SAMIEE S M, FARZAMI M R, ALAVIMAJD H, ZAHEDIRAD M, KALAYI A, SHARIATZADEH N, BOROUMAND N, GOLSHEKAN E, GHOLAMIAN Y, NEYESTANIET T R. J. Clin. Lab. Anal., 2017, 31(6):e22117.NIKOOYEH B, SAMIEE S M, FARZAMI M R, ALAVIMAJD H, ZAHEDIRAD M, KALAYI A, SHARIATZADEH N, BOROUMAND N, GOLSHEKAN E, GHOLAMIAN Y, NEYESTANIET T R. J. Clin. Lab. Anal., 2017, 31(6):e22117.
11. [11]
  LIN J H, YAN F, HU X Y, JU H X. J. Immunol. Methods, 2004, 291:165-174.LIN J H, YAN F, HU X Y, JU H X. J. Immunol. Methods, 2004, 291:165-174.
12. [12]
  LI J J, CHEN X H, WENG G J, ZHU J, ZHAO J W. Mater. Today Commun., 2020, 25:101373.LI J J, CHEN X H, WENG G J, ZHU J, ZHAO J W. Mater. Today Commun., 2020, 25:101373.
13. [13]
  OLOFSSON E, ZENCI V, ATHLIN S. J. Clin. Microbiol., 2019, 57:953.OLOFSSON E, ZENCI V, ATHLIN S. J. Clin. Microbiol., 2019, 57:953.
14. [14]
  WANG Y J, WEI Z K, LUO X D, WAN Q, QIU R L, WANG S Z. Talanta, 2019, 195:33-39.WANG Y J, WEI Z K, LUO X D, WAN Q, QIU R L, WANG S Z. Talanta, 2019, 195:33-39.
15. [15]
  WANG L, LI L, CAO D. Sens. Actuators, B, 2017, 239:1307-1317.WANG L, LI L, CAO D. Sens. Actuators, B, 2017, 239:1307-1317.
16. [16]
  HU Q, DUAN C, WU J J, SU D D, ZENG L T, SHENG R L. Anal. Chem., 2018, 90(14):8686-8691.HU Q, DUAN C, WU J J, SU D D, ZENG L T, SHENG R L. Anal. Chem., 2018, 90(14):8686-8691.
17. [17]
  GONG Y, ZHENG Y M, JIN B R, YOU M L, WANG J Y, LI X J, LIN M, XU F, LI F. Talanta, 2019, 201:126-133.GONG Y, ZHENG Y M, JIN B R, YOU M L, WANG J Y, LI X J, LIN M, XU F, LI F. Talanta, 2019, 201:126-133.
18. [18]
  JIN B R, YANG Y X, HE R Y, PARK Y, LEE A, BAI D, LI F, LU T J, XU F, LIN M. Sens. Actuators, B, 2018, 276:48-56.JIN B R, YANG Y X, HE R Y, PARK Y, LEE A, BAI D, LI F, LU T J, XU F, LIN M. Sens. Actuators, B, 2018, 276:48-56.
19. [19]
  HE W H, YOU M L, LI Z D, CAO L, XU F, LI F, LI A. Sens. Actuators, B, 2021, 334:129673.HE W H, YOU M L, LI Z D, CAO L, XU F, LI F, LI A. Sens. Actuators, B, 2021, 334:129673.
20. [20]
  LIU L, ZHANG H, WANG Z X, SONG D Q. Biosens. Bioelectron., 2019, 141:111403.LIU L, ZHANG H, WANG Z X, SONG D Q. Biosens. Bioelectron., 2019, 141:111403.
21. [21]
  SHANG Y T, XIANG X R, YE Q H, WU Q P, ZHANG J M, LIN J M. TrAC, Trend Anal. Chem., 2022, 147:116509.SHANG Y T, XIANG X R, YE Q H, WU Q P, ZHANG J M, LIN J M. TrAC, Trend Anal. Chem., 2022, 147:116509.
22. [22]
  WU Y M, CHAN S Y, XU J H, LIU X G. Chem.-Asian J., 2021, 16(18):2596-2609.WU Y M, CHAN S Y, XU J H, LIU X G. Chem.-Asian J., 2021, 16(18):2596-2609.
23. [23]
  ANSARI A A, PARCHUR A K, THORAT N D, CHEN G Y. Coordin. Chem. Rev., 2021, 440:213971.ANSARI A A, PARCHUR A K, THORAT N D, CHEN G Y. Coordin. Chem. Rev., 2021, 440:213971.
24. [24]
  MO J, SHEN L, XU Q, ZENG J, SHA J, HU T, BI K, CHEN Y. Nanomaterials, 2019, 9:1700.MO J, SHEN L, XU Q, ZENG J, SHA J, HU T, BI K, CHEN Y. Nanomaterials, 2019, 9:1700.
25. [25]
  DONG H, SUN L D, YAN C H. Front. Chem., 2021, 8:619377.DONG H, SUN L D, YAN C H. Front. Chem., 2021, 8:619377.
26. [26]
  MOON H, KUMAR D, KIM H, SIM C, CHANG J H, KIM J M, KIM H, LIM D K. ACS Nano, 2015, 9(3):2711-2719.MOON H, KUMAR D, KIM H, SIM C, CHANG J H, KIM J M, KIM H, LIM D K. ACS Nano, 2015, 9(3):2711-2719.
27. [27]
  RAO W Y, LI Q, WANG Y Z, LI T, WU L J. ACS Nano, 2015, 9(3):2783-2791.RAO W Y, LI Q, WANG Y Z, LI T, WU L J. ACS Nano, 2015, 9(3):2783-2791.
28. [28]
  NIKOOBAKHT B, EL-SAYED M A. Chem. Mater., 2003, 15(10):1957-1962.NIKOOBAKHT B, EL-SAYED M A. Chem. Mater., 2003, 15(10):1957-1962.
29. [29]
  ZHANG Hua, LI Qun, LIU Gui-Feng. Chin. J. Anal. Chem., 2020, 48(8):1018-1024. 张婳, 李群, 刘桂锋. 分析化学, 2020, 48(8):1018-1024.
30. [30]
  ZHENG J P, CHENG X Z, ZHANG H, BAI X P, AI R Q, SHAO L, WANG J F. Chem. Rev., 2021, 121(21):13342-13453.ZHENG J P, CHENG X Z, ZHANG H, BAI X P, AI R Q, SHAO L, WANG J F. Chem. Rev., 2021, 121(21):13342-13453.
31. [31]
  YUAN F, CHEN H Q, XU J, ZHANG Y Y, WU Y, WANG L. Chem.-Eur. J, 2014, 20(10):2888-2894.YUAN F, CHEN H Q, XU J, ZHANG Y Y, WU Y, WANG L. Chem.-Eur. J, 2014, 20(10):2888-2894.
32. [32]
  LI J J, CHEN X H, WENG G J, ZHU J, ZHAO J W. Mater. Today Commun., 2020, 25:101373.LI J J, CHEN X H, WENG G J, ZHU J, ZHAO J W. Mater. Today Commun., 2020, 25:101373.
33. [33]
  WANG Y J, WEI Z K, LUO X D, WAN Q, QIU R L, WANG S Z. Talanta, 2019, 195:33-39.WANG Y J, WEI Z K, LUO X D, WAN Q, QIU R L, WANG S Z. Talanta, 2019, 195:33-39.
34. [34]
  WU Y W, CHEN X L, LUO X G, YANG M, HOU C J, HUO D Q. Anal. Chim. Acta, 2021, 1183:339000.WU Y W, CHEN X L, LUO X G, YANG M, HOU C J, HUO D Q. Anal. Chim. Acta, 2021, 1183:339000.
35. [35]
  XIANG W W, ZHANG Z J, WENG W Q, WU B D, CHENG J, SHI L, SUN H W, GAO L, SHI K Q. Anal. Chim. Acta, 2020, 1127:156-162.XIANG W W, ZHANG Z J, WENG W Q, WU B D, CHENG J, SHI L, SUN H W, GAO L, SHI K Q. Anal. Chim. Acta, 2020, 1127:156-162.

扫一扫看文章

计量

PDF下载量: 13
文章访问数: 663
HTML全文浏览量: 67

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于上转换纳米粒子-金纳米棒的荧光共振能量转移免疫分析法用于癌胚抗原检测

通讯作者: 刘桂锋,E-mail:gfliu@jlu.edu.cn; 张婳,E-mail:zhanghua@ciac.ac.cn

English

Upconversion Nanoparticles/Gold Nanorods-based Fluorescence Resonance Energy Transfer Immunoassay for Detection of Carcinoembryonic Antigen

Corresponding author: LIU Gui-Feng, ; ZHANG Hua,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于上转换纳米粒子-金纳米棒的荧光共振能量转移免疫分析法用于癌胚抗原检测

通讯作者: 刘桂锋,E-mail:gfliu@jlu.edu.cn; 张婳,E-mail:zhanghua@ciac.ac.cn

English

Upconversion Nanoparticles/Gold Nanorods-based Fluorescence Resonance Energy Transfer Immunoassay for Detection of Carcinoembryonic Antigen

Corresponding author: LIU Gui-Feng, ; ZHANG Hua,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs