Citation: YU Shao-Nan, REN Ling-Ling, REN Li-Qun, BAI Ying-Jie, LIU Gui-Feng, ZHANG Hua, WANG Zhen-Xin. Upconversion Nanoparticles/Gold Nanorods-based Fluorescence Resonance Energy Transfer Immunoassay for Detection of Carcinoembryonic Antigen[J]. Chinese Journal of Analytical Chemistry, ;2022, 50(9): 1299-1307. doi: 10.19756/j.issn.0253-3820.221249 shu

Upconversion Nanoparticles/Gold Nanorods-based Fluorescence Resonance Energy Transfer Immunoassay for Detection of Carcinoembryonic Antigen

1.
Department of Radiology, China-Japan Union Hospital of Jilin University, Changchun 130033, China;
2.
School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China;
3.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Corresponding author: LIU Gui-Feng, ZHANG Hua,
Received Date: 19 May 2022
Revised Date: 15 July 2022

Fund Project: Supported by the Wu Jieping Medical Foundation (Nos.320675019089-40, 320675019089-38) and the National Natural Science Foundation of China (No.61901438).

A fluorescence resonance energy transfer (FRET) immunoassay based on upconversion nanoparticles (UCNP) and gold nanorods (GNR) was constructed for sensitive detection of carcinoembryonic antigen (CEA). UCNP was employed as the donor and GNR was employed as the acceptor. The CEA antibody was covalently attached on the surface of UCNP and GNR to form UCNP-cAb and GNR-dAb conjugates, respectively. After introduction of CEA, a "sandwich" complex was formed through the antigen-antibody specific interaction, which resulted in the close proximity of UCNP and GNR to generate FRET. And the fluorescence quenching efficiency was positively correlated with the concentration of CEA. The constructed FRET immunoassay realized the detection of CEA in both buffer solution and human serum samples with a limit of detection (S/N=3) of 0.01 ng/mL, a linear range from 0.01 ng/mL to 100 ng/mL and good selectivity. It was expected that this FRET immunoassay could be further used in clinical applications and provided new methods for early diagnosis, treatment and prognosis monitoring of related cancers.
- Upconversion nanoparticles,
- Gold nanorods,
- Fluorescence resonance energy transfer,
- Carcinoembryonic antigen,
- Quantitative detection
1. [1]
  CHEN W, ZHENG R, BAADE P D, ZHANG S, ZENG H. CA-Cancer J. Clin., 2016, 66:115-132.
2. [2]
  ALTINTAS Z, TOTHILL I. Sens. Actuators, B, 2013, 188:988-998.
3. [3]
  SHEN G Y, WANG H, DENG T, SHEN G L, YU R Q. Talanta, 2005, 67:217-220.
4. [4]
  GRUNNET M, SORENSEN J B. Lung Cancer, 2012, 76:138-143.
5. [5]
  FERRARI M. Nat. Rev. Cancer, 2005, 5:161-171.
6. [6]
  DUFFY M J. Clin. Chem., 2001, 4:624-630.
7. [7]
  HAIDOPOULOS D, KONSTADOULAKIS M M, ANTONAKIS P T, ALEXIOU D G, MANOURAS A M, KATSARAGAKIS S M, ANDROULAKIS G F. Euro. J. Surg. Oncol., 2000, 26:742-746.
8. [8]
  ZHAO L, XU S, FJAERTOFT G, PAUKSEN K, HAKANSSON L, VENGE P. J. Immunol. Methods, 2004, 293:207-214.
9. [9]
  THOMSON D M, KRUPEY J, FREEDMAN S O, GOLD P. Proc. Natl. Acad. Sci. U.S.A., 1969, 64:161-167.
10. [10]
  NIKOOYEH B, SAMIEE S M, FARZAMI M R, ALAVIMAJD H, ZAHEDIRAD M, KALAYI A, SHARIATZADEH N, BOROUMAND N, GOLSHEKAN E, GHOLAMIAN Y, NEYESTANIET T R. J. Clin. Lab. Anal., 2017, 31(6):e22117.
11. [11]
  LIN J H, YAN F, HU X Y, JU H X. J. Immunol. Methods, 2004, 291:165-174.
12. [12]
  LI J J, CHEN X H, WENG G J, ZHU J, ZHAO J W. Mater. Today Commun., 2020, 25:101373.
13. [13]
  OLOFSSON E, ZENCI V, ATHLIN S. J. Clin. Microbiol., 2019, 57:953.
14. [14]
  WANG Y J, WEI Z K, LUO X D, WAN Q, QIU R L, WANG S Z. Talanta, 2019, 195:33-39.
15. [15]
  WANG L, LI L, CAO D. Sens. Actuators, B, 2017, 239:1307-1317.
16. [16]
  HU Q, DUAN C, WU J J, SU D D, ZENG L T, SHENG R L. Anal. Chem., 2018, 90(14):8686-8691.
17. [17]
  GONG Y, ZHENG Y M, JIN B R, YOU M L, WANG J Y, LI X J, LIN M, XU F, LI F. Talanta, 2019, 201:126-133.
18. [18]
  JIN B R, YANG Y X, HE R Y, PARK Y, LEE A, BAI D, LI F, LU T J, XU F, LIN M. Sens. Actuators, B, 2018, 276:48-56.
19. [19]
  HE W H, YOU M L, LI Z D, CAO L, XU F, LI F, LI A. Sens. Actuators, B, 2021, 334:129673.
20. [20]
  LIU L, ZHANG H, WANG Z X, SONG D Q. Biosens. Bioelectron., 2019, 141:111403.
21. [21]
  SHANG Y T, XIANG X R, YE Q H, WU Q P, ZHANG J M, LIN J M. TrAC, Trend Anal. Chem., 2022, 147:116509.
22. [22]
  WU Y M, CHAN S Y, XU J H, LIU X G. Chem.-Asian J., 2021, 16(18):2596-2609.
23. [23]
  ANSARI A A, PARCHUR A K, THORAT N D, CHEN G Y. Coordin. Chem. Rev., 2021, 440:213971.
24. [24]
  MO J, SHEN L, XU Q, ZENG J, SHA J, HU T, BI K, CHEN Y. Nanomaterials, 2019, 9:1700.
25. [25]
  DONG H, SUN L D, YAN C H. Front. Chem., 2021, 8:619377.
26. [26]
  MOON H, KUMAR D, KIM H, SIM C, CHANG J H, KIM J M, KIM H, LIM D K. ACS Nano, 2015, 9(3):2711-2719.
27. [27]
  RAO W Y, LI Q, WANG Y Z, LI T, WU L J. ACS Nano, 2015, 9(3):2783-2791.
28. [28]
  NIKOOBAKHT B, EL-SAYED M A. Chem. Mater., 2003, 15(10):1957-1962.
29. [29]
30. [30]
  ZHENG J P, CHENG X Z, ZHANG H, BAI X P, AI R Q, SHAO L, WANG J F. Chem. Rev., 2021, 121(21):13342-13453.
31. [31]
  YUAN F, CHEN H Q, XU J, ZHANG Y Y, WU Y, WANG L. Chem.-Eur. J, 2014, 20(10):2888-2894.
32. [32]
  LI J J, CHEN X H, WENG G J, ZHU J, ZHAO J W. Mater. Today Commun., 2020, 25:101373.
33. [33]
  WANG Y J, WEI Z K, LUO X D, WAN Q, QIU R L, WANG S Z. Talanta, 2019, 195:33-39.
34. [34]
  WU Y W, CHEN X L, LUO X G, YANG M, HOU C J, HUO D Q. Anal. Chim. Acta, 2021, 1183:339000.
35. [35]
  XIANG W W, ZHANG Z J, WENG W Q, WU B D, CHENG J, SHI L, SUN H W, GAO L, SHI K Q. Anal. Chim. Acta, 2020, 1127:156-162.
1. [1]
  Hong LI , Xiaoying DING , Cihang LIU , Jinghan ZHANG , Yanying RAO . Detection of iron and copper ions based on gold nanorod etching colorimetry. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 953-962. doi: 10.11862/CJIC.20230370
2. [2]
  Jinghan ZHANG , Guanying CHEN . Progress in the application of rare-earth-doped upconversion nanoprobes in biological detection. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2335-2355. doi: 10.11862/CJIC.20240249
3. [3]
  Lina Liu , Xiaolan Wei , Jianqiang Hu . Exploration of Subject-Oriented Undergraduate Comprehensive Chemistry Experimental Teaching Based on the “STS Concept”: Taking the Experiment of Gold Nanoparticles as an Example. University Chemistry, 2024, 39(10): 337-343. doi: 10.12461/PKU.DXHX202405112
4. [4]
  Yongming Guo , Jie Li , Chaoyong Liu . Green Improvement and Educational Design in the Synthesis and Characterization of Silver Nanoparticles. University Chemistry, 2024, 39(3): 258-265. doi: 10.3866/PKU.DXHX202309057
5. [5]
  Yang YANG , Pengcheng LI , Zhan SHU , Nengrong TU , Zonghua WANG . Plasmon-enhanced upconversion luminescence and application of molecular detection. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 877-884. doi: 10.11862/CJIC.20230440
6. [6]
  Guangming YIN , Huaiyao WANG , Jianhua ZHENG , Xinyue DONG , Jian LI , Yi'nan SUN , Yiming GAO , Bingbing WANG . Preparation and photocatalytic degradation performance of Ag/protonated g-C₃N₄ nanorod materials. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1491-1500. doi: 10.11862/CJIC.20240086
7. [7]
  Min Gu , Huiwen Xiong , Liling Liu , Jilie Kong , Xueen Fang . Rapid Quantitative Detection of Procalcitonin by Microfluidics: An Instrumental Analytical Chemistry Experiment. University Chemistry, 2024, 39(4): 87-93. doi: 10.3866/PKU.DXHX202310120
8. [8]
  Qin Li , Huihui Zhang , Huajun Gu , Yuanyuan Cui , Ruihua Gao , Wei-Lin Dai . In situ Growth of Cd_0.5Zn_0.5S Nanorods on Ti₃C₂ MXene Nanosheet for Efficient Visible-Light-Driven Photocatalytic Hydrogen Evolution. Acta Physico-Chimica Sinica, 2025, 41(4): 100031-. doi: 10.3866/PKU.WHXB202402016
9. [9]
  Liwei Wang , Guangran Ma , Li Wang , Fugang Xu . A Comprehensive Analytical Chemistry Experiment: Colorimetric Detection of Vitamin C Using Nanozyme and Smartphone. University Chemistry, 2024, 39(8): 255-262. doi: 10.3866/PKU.DXHX202312094
10. [10]
  Zijuan LI , Xuan LÜ , Jiaojiao CHEN , Haiyang ZHAO , Shuo SUN , Zhiwu ZHANG , Jianlong ZHANG , Yanling MA , Jie LI , Zixian FENG , Jiahui LIU . Synthesis of visual fluorescence emission CdSe nanocrystals based on ligand regulation. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 308-320. doi: 10.11862/CJIC.20240138
11. [11]
  Feng Lu , Tao Wang , Qi Wang . Preparation and Characterization of Water-Soluble Silver Nanoclusters: A New Design and Teaching Practice in Materials Chemistry Experiment. University Chemistry, 2025, 40(4): 375-381. doi: 10.12461/PKU.DXHX202406005
12. [12]
  Mi Wen , Baoshuo Jia , Yongqi Chai , Tong Wang , Jianbo Liu , Hailong Wu . Improvement of Fluorescence Quantitative Analysis Experiment: Simultaneous Determination of Rhodamine 6G and Rhodamine 123 in Food Using Chemometrics-Assisted Three-Dimensional Fluorescence Method. University Chemistry, 2025, 40(4): 390-398. doi: 10.12461/PKU.DXHX202405147
13. [13]
  Siyi ZHONG , Xiaowen LIN , Jiaxin LIU , Ruyi WANG , Tao LIANG , Zhengfeng DENG , Ao ZHONG , Cuiping HAN . Targeting imaging and detection of ovarian cancer cells based on fluorescent magnetic carbon dots. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1483-1490. doi: 10.11862/CJIC.20240093
14. [14]
  Jiakun BAI , Ting XU , Lu ZHANG , Jiang PENG , Yuqiang LI , Junhui JIA . A red-emitting fluorescent probe with a large Stokes shift for selective detection of hypochlorous acid. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1095-1104. doi: 10.11862/CJIC.20240002
15. [15]
  Qin Hou , Jiayi Hou , Aiju Shi , Xingliang Xu , Yuanhong Zhang , Yijing Li , Juying Hou , Yanfang Wang . Preparation of Cuprous Iodide Coordination Polymer and Fluorescent Detection of Nitrite: A Comprehensive Chemical Design Experiment. University Chemistry, 2024, 39(8): 221-229. doi: 10.3866/PKU.DXHX202312056
16. [16]
  Gaofeng Zeng , Shuyu Liu , Manle Jiang , Yu Wang , Ping Xu , Lei Wang . Micro/Nanorobots for Pollution Detection and Toxic Removal. University Chemistry, 2024, 39(9): 229-234. doi: 10.12461/PKU.DXHX202311055
17. [17]
  Ling Bai , Limin Lu , Xiaoqiang Wang , Dongping Wu , Yansha Gao . Exploration and Practice of Teaching Reforms in “Quantitative Analytical Chemistry” under the Perspective of New Agricultural Science. University Chemistry, 2024, 39(3): 158-166. doi: 10.3866/PKU.DXHX202308101
18. [18]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403
19. [19]
  Guimin ZHANG , Wenjuan MA , Wenqiang DING , Zhengyi FU . Synthesis and catalytic properties of hollow AgPd bimetallic nanospheres. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 963-971. doi: 10.11862/CJIC.20230293
20. [20]
  Yuhao SUN , Qingzhe DONG , Lei ZHAO , Xiaodan JIANG , Hailing GUO , Xianglong MENG , Yongmei GUO . Synthesis and antibacterial properties of silver-loaded sod-based zeolite. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 761-770. doi: 10.11862/CJIC.20230169

Get Citation

PDF

XML

Metrics

PDF Downloads(10)
Abstract views(437)
HTML views(46)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142