基于酶联金纳米复合探针的磁分离免疫传感器检测莱克多巴胺

贺锋 王海洁 李腾飞 杜鹏飞 王维婷 王守经 柳尧波 马艳丽 胡鹏 谭天宇 韩晴

引用本文: 贺锋, 王海洁, 李腾飞, 杜鹏飞, 王维婷, 王守经, 柳尧波, 马艳丽, 胡鹏, 谭天宇, 韩晴. 基于酶联金纳米复合探针的磁分离免疫传感器检测莱克多巴胺[J]. 分析化学, 2023, 51(1): 102-111. doi: 10.19756/j.issn.0253-3820.221241 shu
Citation:  HE Feng,  WANG Hai-Jie,  LI Teng-Fei,  DU Peng-Fei,  WANG Wei-Ting,  WANG Shou-Jing,  LIU Yao-Bo,  MA Yan-Li,  HU Peng,  TAN Tian-Yu,  HAN Qing. Magnetic Separation Immunosensor Based on Enzyme-linked Gold Nanocomposite Probe for Detection of Ractopamine[J]. Chinese Journal of Analytical Chemistry, 2023, 51(1): 102-111. doi: 10.19756/j.issn.0253-3820.221241 shu

基于酶联金纳米复合探针的磁分离免疫传感器检测莱克多巴胺

    通讯作者: 李腾飞,E-mail:litengfei@hebeu.edu.cn; 杜鹏飞,E-mail:dupengfei2011@163.com
  • 基金项目:

    山东省自然科学青年基金项目(No.ZR2020QC250)、国家现代农业(肉羊)产业技术体系建设专项(No.CARS-38)、山东省现代农业产业技术体系-羊创新团队建设项目(No.SDAIT-10-10)和河北省重点研发计划项目(No.22325501D)资助。

摘要: 构建了一种基于新型酶联金纳米复合探针(E-GNPs)的磁分离竞争免疫传感器,用于检测莱克多巴胺(Ractopamine,RAC)。通过链霉亲和素与生物素的特异性相互作用,在抗体上标记辣根过氧化物酶(Horseradish peroxidase,HRP),采用静电组装法将抗体修饰于金纳米颗粒表面得到E-GNPs,引入卵清蛋白(Ovalbumin,OVA)-RAC半抗原(Hapten)复合物包被的磁珠进行竞争反应,通过显色反应实现目标物的定量分析。当目标分子RAC浓度变化时,酶催化底物显色随之改变,并且RAC的浓度与显色信号强度呈线性关系。紫外-可见吸收光谱表征结果表明,一个E-GNPs可携带11个HRP分子,使得该免疫传感器具有较高的灵敏度,检出限低至1.75pg/mL,较传统酶联免疫方法灵敏度提高了10倍。交叉反应实验结果表明,此传感器对RAC的选择性良好,可应用于猪肉、牛肉和羊肉等实际样品中RAC的检测,加标回收率为88.3%~103.4%。本方法为动物源食品中RAC的快速筛查提供了一种新思路。

English


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  • 收稿日期:  2022-05-16
  • 修回日期:  2022-10-07
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